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Literature summary for 1.10.3.2 extracted from
- Biocatalytic properties of cell surface display laccase for degradation of emerging contaminant acetaminophen in water reclamation (2020), Biotechnol. Bioeng., 117, 342-353 .
Application
| Application | Comment | Organism |
|---|---|---|
| environmental protection | the surface display laccase (SDL) biocatalyst, where the enzyme laccase is displayed on the surface of biological cells through synthetic biology, provides a biocatalytic material for removal of emerging contaminants from wastewater | Trametes sp. |
Cloned(Commentary)
| Cloned (Comment) | Organism |
|---|---|
| expression in Saccharomyces cerevisiae EBY100 | Trametes sp. |
General Stability
| General Stability | Organism |
|---|---|
| high stability of the surface display laccase biocatalyst (where the enzyme laccase is displayed on the surface of biological cells through synthetic biology) in reuse | Trametes sp. |
KM Value [mM]
| KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
|---|---|---|---|---|---|
| 0.062 | - |
acetaminophen | pH 5.0, 30°C, surface display laccase (SDL) biocatalyst (where the enzyme laccase is displayed on the surface of biological cells through synthetic biology) | Trametes sp. |  |
| 0.338 | - |
acetaminophen | pH 5.0, 30°C, soluble enzyme | Trametes sp. | |
| 0.394 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 5.0, 30°C, soluble enzyme | Trametes sp. | |
| 0.747 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | pH 5.0, 30°C, surface display laccase (SDL) biocatalyst (where the enzyme laccase is displayed on the surface of biological cells through synthetic biology) | Trametes sp. | |
Organism
| Organism | UniProt | Comment | Textmining |
|---|---|---|---|
| Trametes sp. | - |
- |
- |
Substrates and Products (Substrate)
| Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
|---|---|---|---|---|---|---|
| 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2 | - |
Trametes sp. | ? | - |
? | |
| acetaminophen + O2 | - |
Trametes sp. | ? | - |
? | |
Synonyms
| Synonyms | Comment | Organism |
|---|---|---|
| LAC3 | - |
Trametes sp. |
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Release 2023.1 (January 2023)
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