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Literature summary for 1.10.3.2 extracted from

  • de Salas, F.; Canadas, R.; Santiago, G.; Virseda-Jerez, A.; Vind, J.; Gentili, P.; Martinez, A.T.; Guallar, V.; Munoz, I.G.; Camarero, S.
    Structural and biochemical insights into an engineered high-redox potential laccase overproduced in Aspergillus (2019), Int. J. Biol. Macromol., 141, 855-867 .
    View publication on PubMed
Search for more literature for 1.10.3.2

Cloned(Commentary)

Cloned (Comment) Organism
expression in Saccharomyces cerevisiae basidiomycete PM1

Crystallization (Commentary)

Crystallization (Comment) Organism
hanging-drop vapour-diffusion method, 2.3 A resolution basidiomycete PM1

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0013
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3.0, temperature not specified in the publication basidiomycete PM1
0.002
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3.0, temperature not specified in the publication, wild-type PM1 laccase basidiomycete PM1
0.01
-
 2,6-dimethoxyphenol pH 5.0, temperature not specified in the publication, wild-type PM1 laccase basidiomycete PM1
0.05
-
 2,6-dimethoxyphenol pH 5.0, temperature not specified in the publication basidiomycete PM1
0.24
-
 guaiacol pH 5.0, temperature not specified in the publication, wild-type PM1 laccase basidiomycete PM1
0.29
-
 N,N-Dimethyl-p-phenylenediamine pH 4.0, temperature not specified in the publication basidiomycete PM1
1.04
-
 guaiacol pH 5.0, temperature not specified in the publication basidiomycete PM1
1.06
-
 N,N-Dimethyl-p-phenylenediamine pH 4.0, temperature not specified in the publication, wild-type PM1 laccase basidiomycete PM1
1.41
-
 4-hydroxybenzoic acid pH 5.0, temperature not specified in the publication basidiomycete PM1
1.96
-
 4-hydroxybenzoic acid pH 5.0, temperature not specified in the publication, wild-type PM1 laccase basidiomycete PM1
34.1
-
 1-hydroxybenzotriazole pH 5.0, temperature not specified in the publication basidiomycete PM1
47.7
-
 1-hydroxybenzotriazole pH 5.0, temperature not specified in the publication, wild-type PM1 laccase basidiomycete PM1

Molecular Weight [Da]

Molecular Weight [Da] Molecular Weight Maximum [Da] Comment Organism
65000
-
 MALDI/TOF-TOF analyses, PM1 laccase basidiomycete PM1

Organism

Organism UniProt Comment Textmining
basidiomycete PM1 Q12571
-
-

Posttranslational Modification

Posttranslational Modification Comment Organism
glycoprotein glycosylation degrees around 5% for wild-type enzyme PM1L and 5-17% for mutant enzyme 7D5 basidiomycete PM1

Purification (Commentary)

Purification (Comment) Organism
-
 basidiomycete PM1

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
1-hydroxybenzotriazole + O2
-
 basidiomycete PM1 ?
-
 ?
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + O2
-
 basidiomycete PM1 ?
-
 ?
2,6-dimethoxyphenol + O2
-
 basidiomycete PM1 ?
-
 ?
4-hydroxybenzoic acid + O2
-
 basidiomycete PM1 ?
-
 ?
guaiacol + O2
-
 basidiomycete PM1 ?
-
 ?
N,N-dimethyl-p-phenylenediamine + O2
-
 basidiomycete PM1 ?
-
 ?

Subunits

Subunits Comment Organism
monomer enzyme forms monomers in solution basidiomycete PM1

Synonyms

Synonyms Comment Organism
PM1 laccase
-
 basidiomycete PM1

Temperature Stability [°C]

Temperature Stability Minimum [°C] Temperature Stability Maximum [°C] Comment Organism
50
-
 t1/2: 40.3 h (wild-type PM1 laccase), t1/2: 21.7 h (mutant 7D5 laccase) basidiomycete PM1
60
-
 t1/2: 4.85 h (wild-type PM1 laccase), t1/2: 2.8 h (mutant 7D5 laccase) basidiomycete PM1
70
-
 t1/2: 1.6 h (wild-type PM1 laccase), t1/2: 0.22 h (mutant 7D5 laccase) basidiomycete PM1
75
-
 mutant 7D5 laccase is notably activated at high temperature (laccase activity increases around 60% during the first 5 min of incubation at 75°C) whereas PM1 laccase is not basidiomycete PM1
79
-
 T50: 10 min, wild-type PM1 laccase basidiomycete PM1
80
-
 t1/2: 0.12 h (wild-type PM1 laccase), t1/2: 0.03 h (mutant 7D5 laccase) basidiomycete PM1

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
2.85
-
 guaiacol pH 5.0, temperature not specified in the publication, wild-type PM1 laccase basidiomycete PM1
3.02
-
 4-hydroxybenzoic acid pH 5.0, temperature not specified in the publication, wild-type PM1 laccase basidiomycete PM1
8.6
-
 4-hydroxybenzoic acid pH 5.0, temperature not specified in the publication, engineered enzyme 7D5 basidiomycete PM1
10.5
-
 guaiacol pH 5.0, temperature not specified in the publication, engineered enzyme 7D5 basidiomycete PM1
13.2
-
 2,6-dimethoxyphenol pH 5.0, temperature not specified in the publication, wild-type PM1 laccase basidiomycete PM1
15.5
-
 1-hydroxybenzotriazole pH 5.0, temperature not specified in the publication, wild-type PM1 laccase basidiomycete PM1
28.8
-
 1-hydroxybenzotriazole pH 5.0, temperature not specified in the publication, engineered enzyme 7D5 basidiomycete PM1
44.4
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3.0, temperature not specified in the publication, wild-type PM1 laccase basidiomycete PM1
45.3
-
 2,6-dimethoxyphenol pH 5.0, temperature not specified in the publication, engineered enzyme 7D5 basidiomycete PM1
108.6
-
 N,N-Dimethyl-p-phenylenediamine pH 4.0, temperature not specified in the publication, wild-type PM1 laccase basidiomycete PM1
240
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3.0, temperature not specified in the publication, engineered enzyme 7D5 basidiomycete PM1
938
-
 N,N-Dimethyl-p-phenylenediamine pH 4.0, temperature not specified in the publication, engineered enzyme 7D5 basidiomycete PM1

pH Stability

pH Stability pH Stability Maximum Comment Organism
2
-
 mutant laccase 7D5 at pH 2 is dramatically diminished after 24 h basidiomycete PM1
2
-
 PM1 laccase displays high stability at extreme pH values. It retains 60% of the initial activity after 24 h at pH 2 basidiomycete PM1
9
-
 mutant laccase 7D5 retains 75% activity after 24 h at pH 9 basidiomycete PM1
9
-
 PM1 laccase displays high stability at extreme pH values. It retains 100% of the initial activity after 24 h at pH 9 basidiomycete PM1

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.33
-
 1-hydroxybenzotriazole pH 5.0, temperature not specified in the publication, wild-type PM1 laccase basidiomycete PM1
0.84
-
 1-hydroxybenzotriazole pH 5.0, temperature not specified in the publication basidiomycete PM1
1.54
-
 4-hydroxybenzoic acid pH 5.0, temperature not specified in the publication, wild-type PM1 laccase basidiomycete PM1
6.1
-
 4-hydroxybenzoic acid pH 5.0, temperature not specified in the publication basidiomycete PM1
10.1
-
 guaiacol pH 5.0, temperature not specified in the publication basidiomycete PM1
11.9
-
 guaiacol pH 5.0, temperature not specified in the publication, wild-type PM1 laccase basidiomycete PM1
102
-
 N,N-Dimethyl-p-phenylenediamine pH 4.0, temperature not specified in the publication, wild-type PM1 laccase basidiomycete PM1
905
-
 2,6-dimethoxyphenol pH 5.0, temperature not specified in the publication basidiomycete PM1
1325
-
 2,6-dimethoxyphenol pH 5.0, temperature not specified in the publication, wild-type PM1 laccase basidiomycete PM1
3253
-
 N,N-Dimethyl-p-phenylenediamine pH 4.0, temperature not specified in the publication basidiomycete PM1
26106
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3.0, temperature not specified in the publication, wild-type PM1 laccase basidiomycete PM1
184500
-
 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) pH 3.0, temperature not specified in the publication basidiomycete PM1