BRENDA - Enzyme Database show
show all sequences of 1.10.3.4

Studies on the metabolism of o-aminophenol. Purification and properties of isophenoxazine synthase from Bauhenia monandra

Rao, P.V.S.; Vaidyanathan, C.S.; Arch. Biochem. Biophys. 118, 388-394 (1967)

Data extracted from this reference:

General Stability
General Stability
Organism
stable to extensive dialysis against cyanide or EDTA
Bauhinia monandra
Inhibitors
Inhibitors
Commentary
Organism
Structure
2,3-Dimercaptopropanol
-
Bauhinia monandra
3-hydroxyanthranilic acid
competitive
Bauhinia monandra
Ag+
-
Bauhinia monandra
anthranilic acid
competitive
Bauhinia monandra
ascorbic acid
-
Bauhinia monandra
Atebrin
slightly inhibitory
Bauhinia monandra
azide
-
Bauhinia monandra
Cu2+
-
Bauhinia monandra
cyanide
-
Bauhinia monandra
cysteine
-
Bauhinia monandra
Fe2+
-
Bauhinia monandra
glutathione
-
Bauhinia monandra
Hg2+
-
Bauhinia monandra
N-ethylmaleimide
no effect
Bauhinia monandra
p-hydroxymercuribenzoate
reversed by glutathione or cysteine
Bauhinia monandra
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.75
-
o-aminophenol
-
Bauhinia monandra
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
additional information
no metal requirement
Bauhinia monandra
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Bauhinia monandra
involved in biosynthesis of catechol
?
-
-
-
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bauhinia monandra
-
leguminous plant
-
Purification (Commentary)
Commentary
Organism
-
Bauhinia monandra
Reaction
Reaction
Commentary
Organism
4 2-aminophenol + 3 O2 = 2 2-aminophenoxazin-3-one + 6 H2O
discussion of mechanism, isophenoxazine may be formed by a secondary condensation from the initial oxidation product
Bauhinia monandra
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
56.1
-
-
Bauhinia monandra
Storage Stability
Storage Stability
Organism
-20°C, stable for at least 1 month
Bauhinia monandra
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2 2-aminophenol + 2 O2
highly specific for o-aminophenol, not: related compounds (e.g. 3-hydroxyanthranilic acid, 3-hydroxy-kynurenine, p-aminophenol, catechol)
396432
Bauhinia monandra
isophenoxazine + 2 H2O + H2O2
-
-
-
?
additional information
involved in biosynthesis of catechol
396432
Bauhinia monandra
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
-
Bauhinia monandra
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.2
-
-
Bauhinia monandra
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
no cofactor requirement
Bauhinia monandra
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
no cofactor requirement
Bauhinia monandra
General Stability (protein specific)
General Stability
Organism
stable to extensive dialysis against cyanide or EDTA
Bauhinia monandra
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2,3-Dimercaptopropanol
-
Bauhinia monandra
3-hydroxyanthranilic acid
competitive
Bauhinia monandra
Ag+
-
Bauhinia monandra
anthranilic acid
competitive
Bauhinia monandra
ascorbic acid
-
Bauhinia monandra
Atebrin
slightly inhibitory
Bauhinia monandra
azide
-
Bauhinia monandra
Cu2+
-
Bauhinia monandra
cyanide
-
Bauhinia monandra
cysteine
-
Bauhinia monandra
Fe2+
-
Bauhinia monandra
glutathione
-
Bauhinia monandra
Hg2+
-
Bauhinia monandra
N-ethylmaleimide
no effect
Bauhinia monandra
p-hydroxymercuribenzoate
reversed by glutathione or cysteine
Bauhinia monandra
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.75
-
o-aminophenol
-
Bauhinia monandra
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
additional information
no metal requirement
Bauhinia monandra
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
additional information
Bauhinia monandra
involved in biosynthesis of catechol
?
-
-
-
Purification (Commentary) (protein specific)
Commentary
Organism
-
Bauhinia monandra
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
56.1
-
-
Bauhinia monandra
Storage Stability (protein specific)
Storage Stability
Organism
-20°C, stable for at least 1 month
Bauhinia monandra
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2 2-aminophenol + 2 O2
highly specific for o-aminophenol, not: related compounds (e.g. 3-hydroxyanthranilic acid, 3-hydroxy-kynurenine, p-aminophenol, catechol)
396432
Bauhinia monandra
isophenoxazine + 2 H2O + H2O2
-
-
-
?
additional information
involved in biosynthesis of catechol
396432
Bauhinia monandra
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
40
-
-
Bauhinia monandra
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
6.2
-
-
Bauhinia monandra
Other publictions for EC 1.10.3.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742653
Kumar
-
Structurally characterized mo ...
Streptomyces antibioticus
Inorg. Chim. Acta
464
49-54
2017
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1
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1
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2
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2
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743128
Maji
-
Synthesis, crystal structure, ...
Streptomyces antibioticus
J. Mol. Struct.
1146
821-827
2017
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1
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1
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743665
Ghosh
-
Synthesis, characterization a ...
Streptomyces antibioticus
Polyhedron
123
162-175
2017
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1
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1
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2
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742261
Jia
Preparation and characterizat ...
Streptomyces antibioticus
Chem. Cent. J.
10
34
2016
-
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-
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4
2
-
1
-
1
-
2
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3
2
-
2
-
1
-
2
-
-
-
-
-
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-
-
-
-
-
-
-
4
-
2
-
1
-
1
-
-
-
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3
2
-
2
-
1
-
2
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-
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-
742320
Dey
-
Catechol oxidase and phenoxaz ...
Streptomyces antibioticus
Coord. Chem. Rev.
310
80-115
2016
-
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1
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1
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1
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1
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1
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1
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1
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-
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-
743781
Mitra
-
Catecholase and phenoxazinone ...
Streptomyces antibioticus
RSC Adv.
6
58831-58838
2016
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1
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1
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2
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1
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2
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724838
Jia
-
Optimization of phenoxazinone ...
Streptomyces antibioticus
Electron. J. Biotechnol.
16
0000
2013
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1
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1
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1
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1
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1
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1
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1
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1
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1
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-
713565
Le Roes-Hill
Phenoxazinone synthase: whats' ...
Bauhinia monandra, Streptomyces antibioticus, Streptomyces griseus subsp. griseus, Tecoma stans, Trametes coccinea
Trends Biotechnol.
27
248-258
2009
-
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-
-
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-
49
-
-
4
-
20
-
7
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-
-
-
-
-
-
-
20
-
4
-
-
-
4
-
-
3
-
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-
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-
-
3
-
-
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-
49
-
-
-
4
-
20
-
-
-
-
-
-
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-
20
-
4
-
-
-
4
-
-
-
1
4
4
1
-
-
674768
Suzuki
A novel o-aminophenol oxidase ...
Streptomyces griseus
J. Biol. Chem.
281
824-833
2006
-
-
1
-
-
-
7
6
-
-
1
-
-
4
-
-
1
-
-
-
2
-
12
-
1
-
1
6
1
1
-
-
7
-
-
-
-
1
-
-
-
-
-
7
7
6
-
-
1
-
-
-
-
1
-
-
2
-
12
-
1
-
1
6
1
1
-
-
-
-
-
-
-
-
396432
Rao
Studies on the metabolism of o ...
Bauhinia monandra
Arch. Biochem. Biophys.
118
388-394
1967
-
-
-
-
-
1
15
1
-
1
-
1
-
1
-
-
1
1
-
-
1
1
2
-
1
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
1
-
15
-
1
-
1
-
1
-
-
-
1
-
-
1
1
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
396431
Nair
Isophenoxazine synthase apoenz ...
Trametes coccinea
Biochim. Biophys. Acta
96
318-327
1965
-
-
-
1
-
-
10
1
-
1
-
-
-
2
-
-
1
-
-
1
1
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
1
-
-
-
10
-
1
-
1
-
-
-
-
-
1
-
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
396430
Nair
Isophenoxazine synthase ...
Tecoma stans
Biochim. Biophys. Acta
81
507-516
1964
-
-
-
-
-
-
7
-
-
1
-
-
-
1
-
-
-
-
-
1
-
-
-
-
1
-
-
-
1
1
-
1
-
-
-
-
-
-
1
-
-
-
-
7
-
-
-
1
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-