BRENDA - Enzyme Database show
show all sequences of 1.10.3.4

Optimization of phenoxazinone synthase production by response surface methodology and its application in Congo red decolourization

Jia, H.; Gao, Z.; Ma, Y.; Zhong, C.; Xie, Y.; Zhou, H.; Wei, P.; Electron. J. Biotechnol. 16, 0000 (2013)
No PubMed abstract available

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
expression in Escherichia coli strain BL21(DE3). Optimization of phenoxazinone synthase production by response surface methodology via Box-Behnken design, overview
Streptomyces antibioticus
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Streptomyces antibioticus
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
enzyme from crude cell extract exhibits Congo red decolourization activity at a rate of 27% without mediator. Several mediators improve PHS-catalyzed Congo red decolourization, with the highest rate of 73.89% obtained with 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid as mediator under optimized conditions of 4000 U/l PHS activity, 0.01 mM mediator, 0.1 mM Congo red, and 8 h reaction time. 2,2'-Azinobis-3-ethylbenzothiazoline-6-sulfonic acid is also a substrate for the enzyme
724838
Streptomyces antibioticus
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Streptomyces antibioticus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
-
assay at
Streptomyces antibioticus
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Escherichia coli strain BL21(DE3). Optimization of phenoxazinone synthase production by response surface methodology via Box-Behnken design, overview
Streptomyces antibioticus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
additional information
enzyme from crude cell extract exhibits Congo red decolourization activity at a rate of 27% without mediator. Several mediators improve PHS-catalyzed Congo red decolourization, with the highest rate of 73.89% obtained with 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid as mediator under optimized conditions of 4000 U/l PHS activity, 0.01 mM mediator, 0.1 mM Congo red, and 8 h reaction time. 2,2'-Azinobis-3-ethylbenzothiazoline-6-sulfonic acid is also a substrate for the enzyme
724838
Streptomyces antibioticus
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Streptomyces antibioticus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
5
-
assay at
Streptomyces antibioticus
Other publictions for EC 1.10.3.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
724838
Jia
-
Optimization of phenoxazinone ...
Streptomyces antibioticus
Electron. J. Biotechnol.
16
0000
2013
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1
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1
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1
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1
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1
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1
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1
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1
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713565
Le Roes-Hill
Phenoxazinone synthase: whats' ...
Bauhinia monandra, Pycnoporus coccineus, Streptomyces antibioticus, Streptomyces griseus subsp. griseus, Tecoma stans
Trends Biotechnol.
27
248-258
2009
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49
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4
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20
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7
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20
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4
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4
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3
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3
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49
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4
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20
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20
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4
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4
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1
4
4
1
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674768
Suzuki
A novel o-aminophenol oxidase ...
Streptomyces griseus
J. Biol. Chem.
281
824-833
2006
-
-
1
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7
6
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1
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-
4
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1
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-
-
2
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12
-
1
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1
6
1
1
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7
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1
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7
7
6
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1
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1
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2
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12
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1
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1
6
1
1
-
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396432
Rao
Studies on the metabolism of o ...
Bauhinia monandra
Arch. Biochem. Biophys.
118
388-394
1967
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-
-
-
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1
15
1
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1
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1
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1
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1
1
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1
1
2
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1
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1
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1
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1
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1
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15
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1
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1
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1
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1
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1
1
2
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1
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1
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396431
Nair
Isophenoxazine synthase apoenz ...
Pycnoporus coccineus
Biochim. Biophys. Acta
96
318-327
1965
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-
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1
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10
1
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1
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2
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1
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1
1
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1
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2
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2
1
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10
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1
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1
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1
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1
1
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1
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-
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-
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396430
Nair
Isophenoxazine synthase ...
Tecoma stans
Biochim. Biophys. Acta
81
507-516
1964
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7
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1
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1
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1
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1
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1
1
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1
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1
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7
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1
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1
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1
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1
1
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