Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli strain BL21(DE3). Optimization of phenoxazinone synthase production by response surface methodology via Box-Behnken design, overview | Streptomyces antibioticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Streptomyces antibioticus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | enzyme from crude cell extract exhibits Congo red decolourization activity at a rate of 27% without mediator. Several mediators improve PHS-catalyzed Congo red decolourization, with the highest rate of 73.89% obtained with 2,2'-azinobis-3-ethylbenzothiazoline-6-sulfonic acid as mediator under optimized conditions of 4000 U/l PHS activity, 0.01 mM mediator, 0.1 mM Congo red, and 8 h reaction time. 2,2'-Azinobis-3-ethylbenzothiazoline-6-sulfonic acid is also a substrate for the enzyme | Streptomyces antibioticus | ? | - |
? |
Synonyms | Comment | Organism |
---|---|---|
phenoxazinone synthase | - |
Streptomyces antibioticus |
PHS | - |
Streptomyces antibioticus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Streptomyces antibioticus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
assay at | Streptomyces antibioticus |