BRENDA - Enzyme Database show
show all sequences of 1.10.3.4

Preparation and characterization of a highly stable phenoxazinone synthase nanogel

Jia, H.; Gao, Z.; Ma, Y.; Zhong, C.; Wang, C.; Zhou, H.; Wei, P.; Chem. Cent. J. 10, 34 (2016)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
DMSO
the native enzyme shows less than 5% residual activity in the presence of DMSO
Streptomyces antibioticus
ethanol
the native enzyme shows less than 5% residual activity in the presence of ethanol
Streptomyces antibioticus
ethyl acetate
the native enzyme shows less than 5% residual activity in the presence of ethyl acetate
Streptomyces antibioticus
methanol
the native enzyme shows less than 5% residual activity in the presence of methanol
Streptomyces antibioticus
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.052
-
2-Aminophenol
enzyme on nanogel, at pH 4.5 and 25C
Streptomyces antibioticus
0.077
-
2-Aminophenol
free enzyme, at pH 4.5 and 25C
Streptomyces antibioticus
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Co2+
the enzyme is a multi-copper oxidase
Streptomyces antibioticus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-aminophenol + O2
Streptomyces antibioticus
-
2-aminophenoxazin-3-one + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Streptomyces antibioticus
Q53692
-
-
Storage Stability
Storage Stability
Organism
4C, enzyme on nanogel, 5 weeks, no loss of activity
Streptomyces antibioticus
4C, lyophilized enzyme, 5 weeks, 65% loss of activity
Streptomyces antibioticus
4C, native enzyme at pH 4.5, 5 weeks, 98% loss of activity
Streptomyces antibioticus
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2, 2'-azino-bis(3-ethyl benzothiazoline-6-sulfonic acid) + O2
-
742261
Streptomyces antibioticus
?
-
-
-
?
2-aminophenol + O2
-
742261
Streptomyces antibioticus
2-aminophenoxazin-3-one + H2O
-
-
-
?
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
40
-
free enzyme
Streptomyces antibioticus
50
-
enzyme on nanogel
Streptomyces antibioticus
Temperature Stability [C]
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
60
-
the native enzyme loses about 90% of its activity whereas the enzyme on nanogel loses about 50% of its activity after 2 h preincubation at 60C
Streptomyces antibioticus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4
4.5
enzyme on nanogel
Streptomyces antibioticus
4.5
-
free enzyme
Streptomyces antibioticus
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
DMSO
the native enzyme shows less than 5% residual activity in the presence of DMSO
Streptomyces antibioticus
ethanol
the native enzyme shows less than 5% residual activity in the presence of ethanol
Streptomyces antibioticus
ethyl acetate
the native enzyme shows less than 5% residual activity in the presence of ethyl acetate
Streptomyces antibioticus
methanol
the native enzyme shows less than 5% residual activity in the presence of methanol
Streptomyces antibioticus
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.052
-
2-Aminophenol
enzyme on nanogel, at pH 4.5 and 25C
Streptomyces antibioticus
0.077
-
2-Aminophenol
free enzyme, at pH 4.5 and 25C
Streptomyces antibioticus
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Co2+
the enzyme is a multi-copper oxidase
Streptomyces antibioticus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-aminophenol + O2
Streptomyces antibioticus
-
2-aminophenoxazin-3-one + H2O
-
-
?
Storage Stability (protein specific)
Storage Stability
Organism
4C, enzyme on nanogel, 5 weeks, no loss of activity
Streptomyces antibioticus
4C, lyophilized enzyme, 5 weeks, 65% loss of activity
Streptomyces antibioticus
4C, native enzyme at pH 4.5, 5 weeks, 98% loss of activity
Streptomyces antibioticus
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2, 2'-azino-bis(3-ethyl benzothiazoline-6-sulfonic acid) + O2
-
742261
Streptomyces antibioticus
?
-
-
-
?
2-aminophenol + O2
-
742261
Streptomyces antibioticus
2-aminophenoxazin-3-one + H2O
-
-
-
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
40
-
free enzyme
Streptomyces antibioticus
50
-
enzyme on nanogel
Streptomyces antibioticus
Temperature Stability [C] (protein specific)
Temperature Stability Minimum [C]
Temperature Stability Maximum [C]
Commentary
Organism
60
-
the native enzyme loses about 90% of its activity whereas the enzyme on nanogel loses about 50% of its activity after 2 h preincubation at 60C
Streptomyces antibioticus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
4
4.5
enzyme on nanogel
Streptomyces antibioticus
4.5
-
free enzyme
Streptomyces antibioticus
Other publictions for EC 1.10.3.4
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742653
Kumar
-
Structurally characterized mo ...
Streptomyces antibioticus
Inorg. Chim. Acta
464
49-54
2017
-
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1
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1
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743128
Maji
-
Synthesis, crystal structure, ...
Streptomyces antibioticus
J. Mol. Struct.
1146
821-827
2017
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1
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1
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743665
Ghosh
-
Synthesis, characterization a ...
Streptomyces antibioticus
Polyhedron
123
162-175
2017
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1
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742261
Jia
Preparation and characterizat ...
Streptomyces antibioticus
Chem. Cent. J.
10
34
2016
-
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-
-
-
-
4
2
-
1
-
1
-
2
-
-
-
-
-
-
-
3
2
-
2
-
1
-
2
-
-
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4
-
2
-
1
-
1
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-
-
-
-
-
3
2
-
2
-
1
-
2
-
-
-
-
-
-
-
-
-
742320
Dey
-
Catechol oxidase and phenoxaz ...
Streptomyces antibioticus
Coord. Chem. Rev.
310
80-115
2016
-
-
-
-
-
-
-
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-
1
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1
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1
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1
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1
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1
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1
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-
743781
Mitra
-
Catecholase and phenoxazinone ...
Streptomyces antibioticus
RSC Adv.
6
58831-58838
2016
-
-
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-
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1
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1
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2
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1
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2
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-
724838
Jia
-
Optimization of phenoxazinone ...
Streptomyces antibioticus
Electron. J. Biotechnol.
16
0000
2013
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1
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1
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1
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1
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1
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1
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1
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1
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713565
Le Roes-Hill
Phenoxazinone synthase: whats' ...
Bauhinia monandra, Streptomyces antibioticus, Streptomyces griseus subsp. griseus, Tecoma stans, Trametes coccinea
Trends Biotechnol.
27
248-258
2009
-
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49
-
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4
-
20
-
7
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20
-
4
-
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4
-
-
3
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3
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-
49
-
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4
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20
-
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20
-
4
-
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-
4
-
-
-
1
4
4
1
-
-
674768
Suzuki
A novel o-aminophenol oxidase ...
Streptomyces griseus
J. Biol. Chem.
281
824-833
2006
-
-
1
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-
7
6
-
-
1
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4
-
-
1
-
-
-
2
-
12
-
1
-
1
6
1
1
-
-
7
-
-
-
-
1
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-
-
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-
7
7
6
-
-
1
-
-
-
-
1
-
-
2
-
12
-
1
-
1
6
1
1
-
-
-
-
-
-
-
-
396432
Rao
Studies on the metabolism of o ...
Bauhinia monandra
Arch. Biochem. Biophys.
118
388-394
1967
-
-
-
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-
1
15
1
-
1
-
1
-
1
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1
1
-
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1
1
2
-
1
-
-
-
1
-
-
1
-
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-
-
-
-
1
-
-
1
-
15
-
1
-
1
-
1
-
-
-
1
-
-
1
1
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
396431
Nair
Isophenoxazine synthase apoenz ...
Trametes coccinea
Biochim. Biophys. Acta
96
318-327
1965
-
-
-
1
-
-
10
1
-
1
-
-
-
2
-
-
1
-
-
1
1
-
1
-
-
-
-
-
-
-
-
2
-
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-
-
2
1
-
-
-
10
-
1
-
1
-
-
-
-
-
1
-
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
396430
Nair
Isophenoxazine synthase ...
Tecoma stans
Biochim. Biophys. Acta
81
507-516
1964
-
-
-
-
-
-
7
-
-
1
-
-
-
1
-
-
-
-
-
1
-
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-
-
1
-
-
-
1
1
-
1
-
-
-
-
-
-
1
-
-
-
-
7
-
-
-
1
-
-
-
-
-
-
-
1
-
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-
-
1
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-
-
1
1
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-
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