Application | Comment | Organism |
---|---|---|
degradation | the enzyme can be used for PVC films biodegradation. Fungal metabolites are playing an immense role in developing various sustainable waste treatment processes. Production and characterization of a fungal lignin peroxidase with a potential to degrade polyvinyl chloride, method optimization, overview | Phanerodontia chrysosporium |
Cloned (Comment) | Organism |
---|---|
enzyme cloning from strain NK-1, phylogenetic analysis | Phanerodontia chrysosporium |
Protein Variants | Comment | Organism |
---|---|---|
additional information | fungal metabolites are playing an immense role in developing various sustainable waste treatment processes. Production and characterization of a fungal lignin peroxidase with a potential to degrade polyvinyl chloride, method optimization, overview | Phanerodontia chrysosporium |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
extracellular | - |
Phanerodontia chrysosporium | - |
- |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 veratryl alcohol + H2O2 | Phanerodontia chrysosporium | - |
2 veratryl aldehyde + 2 H2O | - |
? | |
2 veratryl alcohol + H2O2 | Phanerodontia chrysosporium NK-1 | - |
2 veratryl aldehyde + 2 H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Phanerodontia chrysosporium | - |
isolation from a contaminated site that has the waste plastic and wood material in bulk and where the waste materials had been dumped for a long time. Four different fungal colonies, named as Nk-1, Nk-2, Nk-3, and Nk-4, are isolated and purified from the sample | - |
Phanerodontia chrysosporium NK-1 | - |
isolation from a contaminated site that has the waste plastic and wood material in bulk and where the waste materials had been dumped for a long time. Four different fungal colonies, named as Nk-1, Nk-2, Nk-3, and Nk-4, are isolated and purified from the sample | - |
Purification (Comment) | Organism |
---|---|
extracellular enzyme partially from strain NK-1 by ammonium sulfate fractionation and gel filtration | Phanerodontia chrysosporium |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | the isolated sample from waste and contaminated soli is grown on a seaboard dextrose agar medium. Four different fungal colonies, named as Nk-1, Nk-2, Nk-3, and Nk-4, are isolated and purified from the sample. The maximum enzyme production occurs at a temperature 25°C, pH 5.0 in the 4th week of the incubation period with fungal strain, optimization of enzyme production method, overview. Phanerochaete chrysosporium strain NK-1 is able to use PVC as the sole source of carbon and energy | Phanerodontia chrysosporium | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 veratryl alcohol + H2O2 | - |
Phanerodontia chrysosporium | 2 veratryl aldehyde + 2 H2O | - |
? | |
2 veratryl alcohol + H2O2 | - |
Phanerodontia chrysosporium NK-1 | 2 veratryl aldehyde + 2 H2O | - |
? | |
additional information | partially purified lignin peroxidase is used for the degradation of polyvinyl chloride (PVC) films, a significant reduction in the weight of PVC film is observed (31%), measurement of CO2 as reaction product. FTIR spectra of the enzyme-treated plastic film reveal structural changes in the chemical composition, indicating a specific peak at 2943/cm that correspond to alkenyl C-H stretch. Deterioration on the surface of PVC films is confirmed by scanning electron microscopy tracked through activity assay for the lignin peroxidase | Phanerodontia chrysosporium | ? | - |
- |
|
additional information | partially purified lignin peroxidase is used for the degradation of polyvinyl chloride (PVC) films, a significant reduction in the weight of PVC film is observed (31%), measurement of CO2 as reaction product. FTIR spectra of the enzyme-treated plastic film reveal structural changes in the chemical composition, indicating a specific peak at 2943/cm that correspond to alkenyl C-H stretch. Deterioration on the surface of PVC films is confirmed by scanning electron microscopy tracked through activity assay for the lignin peroxidase | Phanerodontia chrysosporium NK-1 | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
? | x * 46000, SDS-PAGE | Phanerodontia chrysosporium |
Synonyms | Comment | Organism |
---|---|---|
fungal lignin peroxidase | - |
Phanerodontia chrysosporium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
25 | - |
assay at | Phanerodontia chrysosporium |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 30 | enzyme activity increases as the temperature increases from 10°C to 30°C in vivo. Further increase in temperature results in an obvious decrease in the amount of enzyme production by the fungal strain | Phanerodontia chrysosporium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5 | - |
- |
Phanerodontia chrysosporium |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
3 | 9 | activity range, optimal activity at pH 5.0, almost inactive at pH 11.0 | Phanerodontia chrysosporium |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
additional information | - |
lignin peroxidase is very stable over a broad range of pH | Phanerodontia chrysosporium |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Phanerodontia chrysosporium |
General Information | Comment | Organism |
---|---|---|
physiological function | Phanerochaete chrysosporium strain NK-1 is able to use polyvinyl chloride (PVC) as the sole source of carbon and energy, and this degradation of the polymer is mainly facilitated by the production of peroxidases. Thus, the enzyme production is considered as the function of cellular growth. Extracellular lignin peroxidases from Phanerocheate chrysosporium play a significant role in the degradation of complex polymeric compounds like PVC | Phanerodontia chrysosporium |