KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.005 | - |
Reactive Black 5 | manganese-independent peroxidase I, at pH 3.0 in sodium tartrate buffer (50 mM), at 20°C | Auricularia auricula-judae | |
0.005 | - |
H2O2 | manganese-independent peroxidase II, using 2,2'-azino-bis(3-ethylthiazoline)-6-sulfonate as cosubstrate, at pH 4.5 in sodium tartrate buffer (50 mM), at 20°C | Auricularia auricula-judae | |
0.007 | - |
Reactive Black 5 | manganese-independent peroxidase II, at pH 3.0 in sodium tartrate buffer (50 mM), at 20°C | Auricularia auricula-judae | |
0.01 | - |
H2O2 | manganese-independent peroxidase I, using 2,2'-azino-bis(3-ethylthiazoline)-6-sulfonate as cosubstrate, at pH 4.5 in sodium tartrate buffer (50 mM), at 20°C | Auricularia auricula-judae | |
0.015 | - |
Reactive Blue 5 | manganese-independent peroxidase II, at pH 3.0 in sodium tartrate buffer (50 mM), at 20°C | Auricularia auricula-judae | |
0.02 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | manganese-independent peroxidase I, at pH 4.5 in sodium tartrate buffer (50 mM), at 20°C | Auricularia auricula-judae | |
0.02 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | manganese-independent peroxidase II, at pH 4.5 in sodium tartrate buffer (50 mM), at 20°C | Auricularia auricula-judae | |
0.023 | - |
Reactive Blue 5 | manganese-independent peroxidase I, at pH 3.0 in sodium tartrate buffer (50 mM), at 20°C | Auricularia auricula-judae | |
0.023 | - |
2,6-dimethoxyphenol | manganese-independent peroxidase II, at pH 4.5 in sodium tartrate buffer (50 mM), at 20°C | Auricularia auricula-judae | |
0.027 | - |
2,6-dimethoxyphenol | manganese-independent peroxidase I, at pH 4.5 in sodium tartrate buffer (50 mM), at 20°C | Auricularia auricula-judae | |
1.779 | - |
veratryl alcohol | manganese-independent peroxidase I, at pH 2.5 in sodium tartrate buffer (50 mM), at 20°C | Auricularia auricula-judae | |
2.89 | - |
veratryl alcohol | manganese-independent peroxidase II, at pH 2.5 in sodium tartrate buffer (50 mM), at 20°C | Auricularia auricula-judae |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | the enzyme shows manganese-independent peroxidase activity | Auricularia auricula-judae |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
manganese-independent peroxidase II, SDS-PAGE | Auricularia auricula-judae |
51000 | - |
manganese-independent peroxidase I, SDS-PAGE | Auricularia auricula-judae |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Auricularia auricula-judae | - |
strain 11326 | - |
Purification (Comment) | Organism |
---|---|
Q Sepharose column chromatography, Mono Q column chromatography, Mono S column chromatography, and Mono P column chromatography | Auricularia auricula-judae |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
28.4 | - |
culture liquid, at pH 3.0 and 20°C | Auricularia auricula-judae |
347.8 | - |
manganese-independent peroxidase II after 12.2fold purification, at pH 3.0 and 20°C | Auricularia auricula-judae |
469 | - |
manganese-independent peroxidase I after 16.5fold purification, at pH 3.0 and 20°C | Auricularia auricula-judae |
Storage Stability | Organism |
---|---|
20°C, manganese-independent peroxidase I in 50 mM sodium tartrate buffer pH 2.5, 24 h, 20% loss of activity | Auricularia auricula-judae |
20°C, manganese-independent peroxidase II in 50 mM sodium tartrate buffer pH 2.5, 24 h, 75% loss of activity | Auricularia auricula-judae |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) + H2O2 + H+ | - |
Auricularia auricula-judae | ? | - |
? | |
2,6-dimethoxyphenol + H2O2 + H+ | - |
Auricularia auricula-judae | ? | - |
? | |
adlerol + H2O2 + H+ | i.e 1-(3,4-dimethoxyphenyl)-1-oxo-2-(2-methoxyphenoxy)-1,3-dihydroxy-propane, maximum oxidation activity at pH 2.5 | Auricularia auricula-judae | ? | - |
? | |
additional information | Mn2+ is not oxidized | Auricularia auricula-judae | ? | - |
? | |
Reactive Black 5 + H2O2 + H+ | - |
Auricularia auricula-judae | oxidized Reactive Black 5 + H2O | - |
? | |
Reactive Blue 5 + H2O2 + H+ | - |
Auricularia auricula-judae | oxidized Reactive Blue 5 + H2O | - |
? | |
veratryl alcohol + H2O2 + H+ | maximum oxidation activity at pH 1.4 | Auricularia auricula-judae | veratraldehyde + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 40000, manganese-independent peroxidase II, SDS-PAGE | Auricularia auricula-judae |
monomer | 1 * 51000, manganese-independent peroxidase I, SDS-PAGE | Auricularia auricula-judae |
Synonyms | Comment | Organism |
---|---|---|
manganese-independent peroxidase I | - |
Auricularia auricula-judae |
manganese-independent peroxidase II | - |
Auricularia auricula-judae |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.62 | - |
veratryl alcohol | manganese-independent peroxidase II, at pH 2.5 in sodium tartrate buffer (50 mM), at 20°C | Auricularia auricula-judae | |
0.86 | - |
veratryl alcohol | manganese-independent peroxidase I, at pH 2.5 in sodium tartrate buffer (50 mM), at 20°C | Auricularia auricula-judae | |
3 | - |
Reactive Black 5 | manganese-independent peroxidase I, at pH 3.0 in sodium tartrate buffer (50 mM), at 20°C | Auricularia auricula-judae | |
3 | - |
Reactive Black 5 | manganese-independent peroxidase II, at pH 3.0 in sodium tartrate buffer (50 mM), at 20°C | Auricularia auricula-judae | |
89 | - |
2,6-dimethoxyphenol | manganese-independent peroxidase II, at pH 4.5 in sodium tartrate buffer (50 mM), at 20°C | Auricularia auricula-judae | |
90 | - |
2,6-dimethoxyphenol | manganese-independent peroxidase I, at pH 4.5 in sodium tartrate buffer (50 mM), at 20°C | Auricularia auricula-judae | |
114 | - |
Reactive Blue 5 | manganese-independent peroxidase I, at pH 3.0 in sodium tartrate buffer (50 mM), at 20°C | Auricularia auricula-judae | |
238 | - |
H2O2 | manganese-independent peroxidase II, using 2,2'-azino-bis(3-ethylthiazoline)-6-sulfonate as cosubstrate, at pH 4.5 in sodium tartrate buffer (50 mM), at 20°C | Auricularia auricula-judae | |
256 | - |
Reactive Blue 5 | manganese-independent peroxidase II, at pH 3.0 in sodium tartrate buffer (50 mM), at 20°C | Auricularia auricula-judae | |
268 | - |
H2O2 | manganese-independent peroxidase I, using 2,2'-azino-bis(3-ethylthiazoline)-6-sulfonate as cosubstrate, at pH 4.5 in sodium tartrate buffer (50 mM), at 20°C | Auricularia auricula-judae | |
322 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | manganese-independent peroxidase II, at pH 4.5 in sodium tartrate buffer (50 mM), at 20°C | Auricularia auricula-judae | |
368 | - |
2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) | manganese-independent peroxidase I, at pH 4.5 in sodium tartrate buffer (50 mM), at 20°C | Auricularia auricula-judae |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
1.4 | - |
maximum activity at pH 1.4 using veratryl alcohol as substrate | Auricularia auricula-judae |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
2.5 | - |
at pH 2.5, manganese-independent peroxidase I is quite stable and does not lose any activity within 4 h, while manganese-independent peroxidase II loses about 40% of its activity within the same time | Auricularia auricula-judae |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | heme-containing enzyme | Auricularia auricula-judae |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Auricularia auricula-judae | manganese-independent peroxidase II, isoelectric focusing | - |
4.1 |
Auricularia auricula-judae | manganese-independent peroxidase I, isoelectric focusing | - |
4.3 |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.22 | - |
veratryl alcohol | manganese-independent peroxidase II, at pH 2.5 in sodium tartrate buffer (50 mM), at 20°C | Auricularia auricula-judae | |
0.48 | - |
veratryl alcohol | manganese-independent peroxidase I, at pH 2.5 in sodium tartrate buffer (50 mM), at 20°C | Auricularia auricula-judae | |
410 | - |
Reactive Black 5 | manganese-independent peroxidase II, at pH 3.0 in sodium tartrate buffer (50 mM), at 20°C | Auricularia auricula-judae | |
570 | - |
Reactive Black 5 | manganese-independent peroxidase I, at pH 3.0 in sodium tartrate buffer (50 mM), at 20°C | Auricularia auricula-judae | |
3300 | - |
2,6-dimethoxyphenol | manganese-independent peroxidase I, at pH 4.5 in sodium tartrate buffer (50 mM), at 20°C | Auricularia auricula-judae | |
3900 | - |
2,6-dimethoxyphenol | manganese-independent peroxidase II, at pH 4.5 in sodium tartrate buffer (50 mM), at 20°C | Auricularia auricula-judae | |
5000 | - |
Reactive Blue 5 | manganese-independent peroxidase I, at pH 3.0 in sodium tartrate buffer (50 mM), at 20°C | Auricularia auricula-judae | |
17000 | - |
Reactive Blue 5 | manganese-independent peroxidase II, at pH 3.0 in sodium tartrate buffer (50 mM), at 20°C | Auricularia auricula-judae | |
27000 | - |
H2O2 | manganese-independent peroxidase I, using 2,2'-azino-bis(3-ethylthiazoline)-6-sulfonate as cosubstrate, at pH 4.5 in sodium tartrate buffer (50 mM), at 20°C | Auricularia auricula-judae | |
48000 | - |
H2O2 | manganese-independent peroxidase II, using 2,2'-azino-bis(3-ethylthiazoline)-6-sulfonate as cosubstrate, at pH 4.5 in sodium tartrate buffer (50 mM), at 20°C | Auricularia auricula-judae |