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Literature summary for 1.11.1.19 extracted from

  • Uchida, T.; Omura, I.; Umetsu, S.; Ishimori, K.
    Radical transfer but not heme distal residues is essential for pH dependence of dye-decolorizing activity of peroxidase from Vibrio cholerae (2021), J. Inorg. Biochem., 219, 111422 .
    View publication on PubMed
Search for more literature for 1.11.1.19

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Vibrio cholerae

Protein Variants

Protein Variants Comment Organism
D138V the mutant displays significantly increased activity at pH 6.5 as compared to the wild type enzyme Vibrio cholerae
D144H almost completely inactive Vibrio cholerae
H178F the mutant exhibits about 35% of wild type activity Vibrio cholerae
H178W inactive Vibrio cholerae
H178Y inactive Vibrio cholerae
T278V the mutant shows wild type activity Vibrio cholerae

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.00053
-
 Reactive Blue 19 mutant enzyme D138V, at pH 6.5 and 25°C Vibrio cholerae
0.0029
-
 Reactive Blue 19 mutant enzyme D138V, at pH 4.0 and 25°C Vibrio cholerae
0.03
-
 Reactive Blue 19 wild type enzyme, at pH 4.5 and 25°C Vibrio cholerae
0.066
-
 Reactive Blue 19 wild type enzyme, at pH 7.0 and 25°C Vibrio cholerae

Organism

Organism UniProt Comment Textmining
Vibrio cholerae
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography and Superdex 200pg gel filtration Vibrio cholerae

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
Reactive Blue 19 + H2O2 + H+ i.e. disodium;1-amino-9,10-dioxo-4-[3-(2-sulfonatooxyethylsulfonyl)anilino]anthracene-2-sulfonate Vibrio cholerae oxidized Reactive Blue 19 + H2O
-
 ?

Synonyms

Synonyms Comment Organism
dye-decolorizing peroxidase
-
 Vibrio cholerae
DyP
-
 Vibrio cholerae

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.014
-
 Reactive Blue 19 wild type enzyme, at pH 7.0 and 25°C Vibrio cholerae
0.17
-
 Reactive Blue 19 mutant enzyme D138V, at pH 4.0 and 25°C Vibrio cholerae
0.47
-
 Reactive Blue 19 mutant enzyme D138V, at pH 6.5 and 25°C Vibrio cholerae
2.3
-
 Reactive Blue 19 wild type enzyme, at pH 4.5 and 25°C Vibrio cholerae

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
4.5
-
-
 Vibrio cholerae

Cofactor

Cofactor Comment Organism Structure
heme
-
 Vibrio cholerae

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.21
-
 Reactive Blue 19 wild type enzyme, at pH 7.0 and 25°C Vibrio cholerae
59
-
 Reactive Blue 19 mutant enzyme D138V, at pH 4.0 and 25°C Vibrio cholerae
77
-
 Reactive Blue 19 wild type enzyme, at pH 4.5 and 25°C Vibrio cholerae
890
-
 Reactive Blue 19 mutant enzyme D138V, at pH 6.5 and 25°C Vibrio cholerae