Literature summary for 1.11.1.19 extracted from

Habib, M.; Rozeboom, H.; Fraaije, M.
Characterization of a new DyP-peroxidase from the alkaliphilic cellulomonad, Cellulomonas bogoriensis (2019), Molecules, 24, 1208 .

Search for more literature for 1.11.1.19

Cloned(Commentary)

Cloned (Comment)	Organism
expressed in Escherichia coli NEB 10beta cells	Cellulomonas bogoriensis

Crystallization (Commentary)

Crystallization (Comment)	Organism
sitting drop vapor diffusion method, using 20% (w/v) PEG300, 0.1 M Tris buffer pH 8.5, 5% PEG8000 and 10% (v/v) glycerol	Cellulomonas bogoriensis

Protein Variants

Protein Variants	Comment	Organism
E201D	the mutant shows a significantly higher activity on several dyes when compared with the wild type enzyme and the activity of the mutant enzyme is almost 10fold greater than the wild type at pH 4.0	Cellulomonas bogoriensis

KM Value [mM]

KM Value [mM]	KM Value Maximum [mM]	Substrate	Comment	Organism	Structure
0.011	-	H2O2	mutant enzyme E201D, with Reactive Blue 19 as cosubstrate, at pH 5.0, temperature not specified in the publication	Cellulomonas bogoriensis
0.017	-	Reactive Blue 19	wild type enzyme, at pH 5.0, temperature not specified in the publication	Cellulomonas bogoriensis
0.033	-	H2O2	wild type enzyme, with Reactive Blue 19 as cosubstrate, at pH 5.0, temperature not specified in the publication	Cellulomonas bogoriensis
0.036	-	Reactive Blue 19	mutant enzyme E201D, at pH 5.0, temperature not specified in the publication	Cellulomonas bogoriensis

Organism

Organism	UniProt	Comment	Textmining
Cellulomonas bogoriensis	-	-	-

Purification (Commentary)

Purification (Comment)	Organism
Ni-Sepharose column chromatography, and Superdex 200 gel filtration	Cellulomonas bogoriensis

Substrates and Products (Substrate)

Substrates	Comment Substrates	Organism	Products	Comment (Products)	Rev.	Reac.
Acid Red 14 + H2O2 + H+	-	Cellulomonas bogoriensis	oxidized Acid Red 14 + H2O	-	?
Disperse Blue 1 + H2O2 + H+	-	Cellulomonas bogoriensis	oxidized Disperse Blue 1 + H2O	-	?
indigotetrasulfonate + H2O2 + H+	-	Cellulomonas bogoriensis	oxidized indigotetrasulfonate + H2O	-	?
Reactive Blue 19 + H2O2 + H+	highest activity	Cellulomonas bogoriensis	oxidized Reactive Blue 19 + H2O	-	?

Subunits

Subunits	Comment	Organism
homodimer	-	Cellulomonas bogoriensis

Synonyms

Synonyms	Comment	Organism
DyP	-	Cellulomonas bogoriensis
DyP-type peroxidase	-	Cellulomonas bogoriensis

Temperature Stability [°C]

Temperature Stability Minimum [°C]	Temperature Stability Maximum [°C]	Comment	Organism
44.8	-	melting temperature	Cellulomonas bogoriensis

Turnover Number [1/s]

Turnover Number Minimum [1/s]	Turnover Number Maximum [1/s]	Substrate	Comment	Organism	Structure
0.22	-	Reactive Blue 19	wild type enzyme, at pH 5.0, temperature not specified in the publication	Cellulomonas bogoriensis
2.88	-	Reactive Blue 19	mutant enzyme E201D, at pH 5.0, temperature not specified in the publication	Cellulomonas bogoriensis

pH Optimum

pH Optimum Minimum	pH Optimum Maximum	Comment	Organism
5	-	-	Cellulomonas bogoriensis

Cofactor

Cofactor	Comment	Organism	Structure
heme	-	Cellulomonas bogoriensis

kcat/KM [mM/s]

kcat/KM Value [1/mMs^-1]	kcat/KM Value Maximum [1/mMs^-1]	Substrate	Comment	Organism	Structure
13	-	Reactive Blue 19	wild type enzyme, at pH 5.0, temperature not specified in the publication	Cellulomonas bogoriensis
80	-	Reactive Blue 19	mutant enzyme E201D, at pH 5.0, temperature not specified in the publication	Cellulomonas bogoriensis

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Release 2023.1 (January 2023)