Activating Compound | Comment | Organism | Structure |
---|---|---|---|
3-Amino-1,2,4-triazole | 156% relative catalase activity at 10 mM, 121% relative peroxidase activity at 10 mM | Mycobacterium sp. |
General Stability | Organism |
---|---|
the enzyme retains both catalase and peroxidase activities after having been subjected to PAGE in the presence of 0.1% (w/v) SDS, over 90% of the initial catalase and peroxidase activities are detected from enzyme preparations incubated for 5 min in an ethanol-chloroform mixture | Mycobacterium sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
hydroxylamine | complete inhibition of catalase activity at 1 mM, 42% residual peroxidase activity at 1 mM | Mycobacterium sp. | |
isoniazid | - |
Mycobacterium sp. | |
KCN | 4% residual catalase activity at 1 mM, 4% residual peroxidase activity at 1 mM | Mycobacterium sp. | |
additional information | the enzyme is resistant to inhibition by 3-amino-1,2,4-triazole | Mycobacterium sp. | |
NaN3 | 8% residual catalase activity at 1 mM, 1% residual peroxidase activity at 1 mM | Mycobacterium sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0478 | - |
o-Dianisidine | peroxidase activity, in 50 mM potassium phosphate buffer (pH 7.0), at 30°C | Mycobacterium sp. | |
1.47 | - |
H2O2 | peroxidase activity, in 50 mM potassium phosphate buffer (pH 7.0), at 30°C | Mycobacterium sp. | |
6.3 | - |
H2O2 | catalase activity, in 50 mM potassium phosphate buffer (pH 7.0), at 30°C | Mycobacterium sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
80000 | - |
2 * 80000, SDS-PAGE | Mycobacterium sp. |
160000 | - |
gel filtration | Mycobacterium sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Mycobacterium sp. | - |
formerly Acinetobacter sp. | - |
Mycobacterium sp. JC1 DSM 3803 | - |
formerly Acinetobacter sp. | - |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, Sepharose 6B column chromatography, DEAE-Sephacel column chromatography, Sephadex G-25 gel filtration, and hydroxylapatite column chromatography | Mycobacterium sp. |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
culture condition:methanol-grown cell | - |
Mycobacterium sp. | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
24.2 | - |
crude extract, in 50 mM potassium phosphate buffer (pH 7.0), at 30°C | Mycobacterium sp. |
1145 | - |
after 47.3fold purification, in 50 mM potassium phosphate buffer (pH 7.0), at 30°C | Mycobacterium sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
H2O2 | - |
Mycobacterium sp. | O2 + H2O | - |
? | |
H2O2 | - |
Mycobacterium sp. DSM 3803 / JC1 | O2 + H2O | - |
? | |
H2O2 | - |
Mycobacterium sp. JC1 DSM 3803 | O2 + H2O | - |
? | |
methanol + H2O2 | at pH 4.5 | Mycobacterium sp. | formaldehyde + H2O | - |
? | |
NADH + H2O2 | - |
Mycobacterium sp. | ? | - |
? | |
o-dianisidine + H2O2 | - |
Mycobacterium sp. | oxidized o-dianisidine + H2O | - |
? | |
o-dianisidine + H2O2 | - |
Mycobacterium sp. DSM 3803 / JC1 | oxidized o-dianisidine + H2O | - |
? | |
o-dianisidine + H2O2 | - |
Mycobacterium sp. JC1 DSM 3803 | oxidized o-dianisidine + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 80000, SDS-PAGE | Mycobacterium sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
peroxidase activity | Mycobacterium sp. |
40 | - |
catalase activity | Mycobacterium sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
60 | - |
when the purified enzyme is incubated for 1 min at 60°C, catalase and peroxidase activities decrease by over 80% | Mycobacterium sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
4 | - |
peroxidase activity | Mycobacterium sp. |
7 | - |
catalase activity | Mycobacterium sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | - |
Mycobacterium sp. |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Mycobacterium sp. | isoelectric focusing | - |
4.8 |
Organism | Comment | Expression |
---|---|---|
Mycobacterium sp. | the peroxidase activity of catalase-peroxidase in cells growing at the late-exponential growth phase (24 h after inoculation) decreases to 25% less than that in cells growing in the absence of isoniazid | down |
Mycobacterium sp. | isoniazid had almost no effect on the expression of catalase-peroxidase | additional information |
Mycobacterium sp. | the peroxidase activity of catalase-peroxidase in sodium nitroprusside-treated cells is 2.7fold higher than that in cells not treated with 1 mM sodium nitroprusside | up |