Activating Compound | Comment | Organism | Structure |
---|---|---|---|
EDTA | 2 mM, 128% of initial activity | Camelus dromedarius | |
NaN3 | 2 mM, 131% of initial activity | Camelus dromedarius | |
PMSF | 2 mM, 167% of initial activity | Camelus dromedarius | |
SDS | 2 mM, 154% of initial activity | Camelus dromedarius |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | 2 mM, 23% residual activity | Camelus dromedarius | |
DL-dithiothreitol | 2 mM, no residual activity | Camelus dromedarius | |
Fe2+ | 2 mM, 21% of initial activity | Camelus dromedarius |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 2 mM, 147% of initial activity | Camelus dromedarius | |
Ca2+ | 2 mM, 150% of initial activity | Camelus dromedarius | |
Co2+ | 2 mM, 184% of initial activity | Camelus dromedarius | |
Mg2+ | 2 mM, 154% of initial activity | Camelus dromedarius | |
selenium | 3.06 mol of selenium per mol of protein | Camelus dromedarius | |
Zn2+ | 2 mM, 166% of initial activity | Camelus dromedarius |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
69000 | - |
gel filtration | Camelus dromedarius |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Camelus dromedarius | - |
- |
- |
Purification (Comment) | Organism |
---|---|
by ethanol-chloroform treatment, acetone precipitation and ion exchange chromatography | Camelus dromedarius |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
erythrocyte | - |
Camelus dromedarius | - |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
12.09 | - |
pH 7, 37°C | Camelus dromedarius |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2 glutathione + cumene hydroperoxide | 83% of the activity with H2O2 | Camelus dromedarius | glutathione disulfide + 2-phenylpropan-2-ol + H2O | - |
? | |
2 glutathione + H2O2 | - |
Camelus dromedarius | glutathione disulfide + H2O | - |
? | |
2 glutathione + tert-butyl hydroperoxide | 87% of the activity with H2O2 | Camelus dromedarius | glutathione disulfide + tert-butyl alcohol + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | * 53000 and x * 21000, SDS-PAGE | Camelus dromedarius |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
47 | - |
- |
Camelus dromedarius |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.2 | - |
- |
Camelus dromedarius |