Activating Compound | Comment | Organism | Structure |
---|---|---|---|
D-penicillamine | addition of D-penicillamine significantly enhances the rate of chloroacetonitrile oxidation and cyanide release by the myeloperoxidase/H2O2/Cl- system (17.2% increase at 5 mM) | Homo sapiens | |
glutathione | addition of glutathione significantly enhances the rate of chloroacetonitrile oxidation and cyanide release by the myeloperoxidase/H2O2/Cl- system (24% increase at 5 mM) | Homo sapiens | |
L-cysteine | addition of L-cysteine significantly enhances the rate of chloroacetonitrile oxidation and cyanide release by the myeloperoxidase/H2O2/Cl- system (45.4% increase at 5 mM) | Homo sapiens | |
N-acetyl-L-cysteine | addition of N-acetyl-L-cysteine significantly enhances the rate of chloroacetonitrile oxidation and cyanide release by the myeloperoxidase/H2O2/Cl- system (16.3% increase at 5 mM) | Homo sapiens |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
4-aminobenzoic acid hydrazine | metabolism of chloroacetonitrile to cyanide by MPO/H2O2/Cl- system is significantly reduced by 4-amino benzoic acid hydrazine to 52.6% of control value | Homo sapiens | |
catalase | addition of the antioxidant enzyme catalase to incubation mixtures results in 56.1% decrease in cyanide release by MPO/H2O2/Cl- system | Homo sapiens | |
indomethacin | metabolism of chloroacetonitrile to cyanide by MPO/H2O2/Cl- system is significantly reduced by indomethacin to 88.9% of control value | Homo sapiens | |
Sodium azide | metabolism of chloroacetonitrile to cyanide by MPO/H2O2/Cl- system is significantly reduced by sodium azide to 43.9% of control value | Homo sapiens |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.089 | - |
Chloroacetonitrile | at pH 5.5, 37°C, 0.1 mM KCl, 0.5 mM H2O2, 0.15 mM chloroacetonitrile, 5 units/ml MPO, and 0.25 mM NaOCl during an incubation period of 60 min | Homo sapiens |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Homo sapiens | - |
- |
- |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leukocyte | - |
Homo sapiens | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
Cl- + H2O2 + chloroacetonitrile | chloride ion (Cl-) is the dominant electron donor, chloroacetonitrile is activated to cyanide by myeloperoxidase/H2O2/Cl- system in vitro | Homo sapiens | HClO + H2O + cyanide | - |
? |
Synonyms | Comment | Organism |
---|---|---|
MPO | - |
Homo sapiens |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
optimum temperature for the activation of chloroacetonitrile to cyanide by the myeloperoxidase/H2O2/Cl- system | Homo sapiens |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
5.5 | - |
optimum pH for the activation of chloroacetonitrile to cyanide by the myeloperoxidase/H2O2/Cl- system | Homo sapiens |