Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 0.5 mM, complete inhibition | Pseudomonas aeruginosa | |
Cu2+ | 0.5 mM, 81% inhibition | Pseudomonas aeruginosa | |
Mn2+ | 0.5 mM, 64% inhibition | Pseudomonas aeruginosa | |
PCMB | 0.5 mM, complete inhibition | Pseudomonas aeruginosa |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0032 | - |
3-methylcatechol | - |
Pseudomonas aeruginosa | |
0.0059 | - |
catechol | - |
Pseudomonas aeruginosa | |
0.013 | - |
4-methylcatechol | - |
Pseudomonas aeruginosa |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
intracellular | - |
Pseudomonas aeruginosa | 5622 | - |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
22000 | - |
gel filtration | Pseudomonas aeruginosa |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
TKU002 | - |
Pseudomonas aeruginosa TKU002 | - |
TKU002 | - |
Purification (Comment) | Organism |
---|---|
- |
Pseudomonas aeruginosa |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
10 | - |
- |
Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-methylcatechol + O2 | 10% of the activity with catechol | Pseudomonas aeruginosa | 2-methyl-cis,cis-muconate | - |
? | |
3-methylcatechol + O2 | 10% of the activity with catechol | Pseudomonas aeruginosa TKU002 | 2-methyl-cis,cis-muconate | - |
? | |
4-chlorocatechol + O2 | 11% of the activity with catechol | Pseudomonas aeruginosa | (2E,4Z)-3-chlorohexa-2,4-dienedioate | - |
? | |
4-chlorocatechol + O2 | 11% of the activity with catechol | Pseudomonas aeruginosa TKU002 | (2E,4Z)-3-chlorohexa-2,4-dienedioate | - |
? | |
4-methylcatechol + O2 | 43% of the activity with catechol | Pseudomonas aeruginosa | 3-methyl-cis,cis-muconate | - |
? | |
4-methylcatechol + O2 | 43% of the activity with catechol | Pseudomonas aeruginosa TKU002 | 3-methyl-cis,cis-muconate | - |
? | |
catechol + O2 | - |
Pseudomonas aeruginosa | cis,cis-muconate | - |
? | |
catechol + O2 | - |
Pseudomonas aeruginosa TKU002 | cis,cis-muconate | - |
? | |
pyrogallol + O2 | 180% of the activity with catechol | Pseudomonas aeruginosa | alpha-hydroxymuconic acid | - |
? | |
pyrogallol + O2 | 180% of the activity with catechol | Pseudomonas aeruginosa TKU002 | alpha-hydroxymuconic acid | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 22000, SDS-PAGE | Pseudomonas aeruginosa |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Pseudomonas aeruginosa |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
20 | 50 | 20°C: about 30% of maximal activity, 50°C: about 20% of maximal activity | Pseudomonas aeruginosa |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
1 h, stable up to | Pseudomonas aeruginosa |
50 | - |
1 h, 90% loss of activity | Pseudomonas aeruginosa |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
1.1 | - |
3-methylcatechol | - |
Pseudomonas aeruginosa | |
5.6 | - |
4-methylcatechol | - |
Pseudomonas aeruginosa | |
7.2 | - |
catechol | - |
Pseudomonas aeruginosa |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Pseudomonas aeruginosa |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 10 | pH 6.5: about 25% of maximal activity, pH 10: about 70% of maximal activity | Pseudomonas aeruginosa |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
6 | - |
4°C, 24 h, about 60% loss of activity | Pseudomonas aeruginosa |
7 | 9 | 4°C, 24 h, stable | Pseudomonas aeruginosa |
10 | - |
4°C, 24 h, complete loss of activity | Pseudomonas aeruginosa |
Organism | Comment | pI Value Maximum | pI Value |
---|---|---|---|
Pseudomonas aeruginosa | isoelectric point is lower than 4.0, chromatofocusing | - |
additional information |