Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Acidithiobacillus caldus |
Protein Variants | Comment | Organism |
---|---|---|
D130A | mutation in putative iron ligand, 0.5-2% residual activity | Acidithiobacillus caldus |
D130E | mutation in putative iron ligand, 0.5-2% residual activity | Acidithiobacillus caldus |
D130H | mutation in putative iron ligand, 0.5-2% residual activity | Acidithiobacillus caldus |
D141A | inactive concomitant to a decrease of melting point by 3-8 K | Acidithiobacillus caldus |
H113A | mutation in putative iron ligand, inactive | Acidithiobacillus caldus |
H113G | mutation in putative iron ligand, inactive | Acidithiobacillus caldus |
H171A | inactive concomitant to a decrease of melting point by 3-8 K | Acidithiobacillus caldus |
H57A | mutation in putative iron ligand, 6% residual activity | Acidithiobacillus caldus |
H57G | mutation in putative iron ligand, inactive | Acidithiobacillus caldus |
additional information | residues located in the predicted GSH/GSSH binding site and in the central hydrogen bond networks including the iron ligands are essential for activity | Acidithiobacillus caldus |
R139A | inactive concomitant to a decrease of melting point by 3-8 K | Acidithiobacillus caldus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
iodoacetamide | - |
Acidithiobacillus caldus | |
N-ethylmaleimide | - |
Acidithiobacillus caldus | |
tris(2-carboxyethyl)phosphine | - |
Acidithiobacillus caldus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.5 | - |
glutathione | pH 7.5, 40°C | Acidithiobacillus caldus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | 0.77 mol per mol of subunit | Acidithiobacillus caldus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
54000 | - |
PAGE | Acidithiobacillus caldus |
99400 | - |
gel filtration | Acidithiobacillus caldus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acidithiobacillus caldus | A0A2P9DTT9 | - |
- |
Posttranslational Modification | Comment | Organism |
---|---|---|
additional information | residues C87 and C224 form a disulfide bridge | Acidithiobacillus caldus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
111.5 | - |
40°C, pH 7.5 | Acidithiobacillus caldus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
glutathione + sulfite + 2 H+ | - |
Acidithiobacillus caldus | S-sulfanylglutathione + O2 + H2O | - |
r | |
S-sulfanylglutathione + O2 + H2O | - |
Acidithiobacillus caldus | glutathione + sulfite + 2 H+ | - |
r |
Subunits | Comment | Organism |
---|---|---|
dimer | and tetramer, 4 * 27000, SDS-PAGE | Acidithiobacillus caldus |
tetramer | and dimer, 4 * 27000, SDS-PAGE | Acidithiobacillus caldus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | 45 | - |
Acidithiobacillus caldus |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
63 | - |
melting temperature | Acidithiobacillus caldus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
181 | - |
glutathione | pH 7.5, 40°C | Acidithiobacillus caldus | |
198 | - |
S-sulfanylglutathione | sigmoidal curve with Hill coefficient 2.3, pH 7.5, 40°C | Acidithiobacillus caldus |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
361 | - |
glutathione | pH 7.5, 40°C | Acidithiobacillus caldus |