Cloned (Comment) | Organism |
---|---|
expressed as a His-tag fusion protein in Escherichia coli BL21(DE3)pLysS | Rattus norvegicus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
homocysteine | 50% inhibition at 6.5 mM | Rattus norvegicus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
2.5 | - |
L-cysteine | pH 7.5, 37°C | Rattus norvegicus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | loosely bound to protein, only 10% of purified protein contains iron | Rattus norvegicus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
24950 | - |
mass spectrometry, thrombin cleaved enzyme | Rattus norvegicus |
26800 | - |
His-tag fusion protein | Rattus norvegicus |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-cysteine + O2 | Rattus norvegicus | key enzyme of cysteine catabolism, supplies substrate for taurine biosynthesis | 3-sulfino-L-alanine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rattus norvegicus | - |
- |
- |
Purification (Comment) | Organism |
---|---|
recombinant protein using His-tag | Rattus norvegicus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-cysteine + O2 | - |
Rattus norvegicus | 3-sulfino-L-alanine | - |
? | |
L-cysteine + O2 | key enzyme of cysteine catabolism, supplies substrate for taurine biosynthesis | Rattus norvegicus | 3-sulfino-L-alanine | - |
? |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Rattus norvegicus |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6.5 | 9.5 | - |
Rattus norvegicus |