Application | Comment | Organism |
---|---|---|
agriculture | plant 4-hydroxyphenylpyruvate dioxygenase (HPPD) is the molecular target of a range of commercial synthetic beta-triketone herbicides Their mode of action is based on an irreversible inhibition of HPPD | Arabidopsis thaliana |
drug development | plant 4-hydroxyphenylpyruvate dioxygenase (HPPD) is the molecular target for development of specific inhibitors | Arabidopsis thaliana |
Cloned (Comment) | Organism |
---|---|
gene HPD, recombinant expression of N-terminally His6-tagged enzyme in Escherichia coli strain BL21(DE3), subcloning in Escherichia coli strain DH5alpha, determination of protein expression patterns | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
leptospermone | - |
Arabidopsis thaliana | |
mesotrione | - |
Arabidopsis thaliana | |
additional information | high-throughput screening of 4-hydroxyphenylpyruvate dioxygenase inhibitors | Arabidopsis thaliana | |
sulcotrione | - |
Arabidopsis thaliana | |
tembotrione | - |
Arabidopsis thaliana |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxyphenylpyruvate + O2 | Arabidopsis thaliana | - |
homogentisate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | P93836 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged enzyme from Escherichia coli strain BL21(DE3) by nickel affinity chromatography | Arabidopsis thaliana |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxyphenylpyruvate + O2 | - |
Arabidopsis thaliana | homogentisate + CO2 | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 48000, recombinant His-tagged enzyme, SDS-PAGE | Arabidopsis thaliana |
Synonyms | Comment | Organism |
---|---|---|
HPD | - |
Arabidopsis thaliana |
HPPD | - |
Arabidopsis thaliana |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
assay at | Arabidopsis thaliana |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.6 | - |
assay at | Arabidopsis thaliana |