Crystallization (Comment) | Organism |
---|---|
a ligand-mediated conformational switch in helix H11 mediates rapid substrate access followed by active site closing and efficient product release through H11 opening. In mutant HPPD, large differences in H11 gating are found with correlation to experimentally measured herbicide tolerance | Zea mays |
a ligand-mediated conformational switch in helix H11 mediates rapid substrate access followed by active site closing and efficient product release through H11 opening. In mutant HPPD, large differences in H11 gating are found with correlation to experimentally measured herbicide tolerance | Arabidopsis thaliana |
Protein Variants | Comment | Organism |
---|---|---|
M335H/P336A/E363Q | residues 336 and 363 are substituted by the corresponding residues of Zea mays HPPD, more than twofold increase in IC50 for sulcotrione and tembotrione. Increased herbicide tolerance compared to wild-type matches with a clear shift in the free energy profile toward favoring the closed helix H11 state | Zea mays |
M335H/P336A/E363Q | residues 336 and 363 are substituted by the corresponding residues of Zea mays HPPD, more than twofold increase in IC50 for sulcotrione and tembotrione. Increased herbicide tolerance compared to wild-type matches with a clear shift in the free energy profile toward favoring the closed helix H11 state | Arabidopsis thaliana |
M335H/P336A/P339T/T341D/E363Q/E426Q | substitutions correspond to residues found in Zea mays. The mutant has a clear preference for the closed H11 state | Zea mays |
M335H/P336A/P339T/T341D/E363Q/E426Q | substitutions correspond to residues found in Zea mays. The mutant has a clear preference for the closed H11 state | Arabidopsis thaliana |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
bicyclopyrone | - |
Arabidopsis thaliana | |
bicyclopyrone | - |
Zea mays | |
isoxaflutole | - |
Arabidopsis thaliana | |
isoxaflutole | - |
Zea mays | |
mesotrione | - |
Arabidopsis thaliana | |
mesotrione | - |
Zea mays | |
sulcotrione | - |
Arabidopsis thaliana | |
sulcotrione | - |
Zea mays | |
tembotrione | - |
Arabidopsis thaliana | |
tembotrione | - |
Zea mays | |
topramezone | - |
Arabidopsis thaliana | |
topramezone | - |
Zea mays |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.077 | - |
4-hydroxyphenylpyruvate | wild-type, pH 7,20°C | Arabidopsis thaliana | |
0.098 | - |
4-hydroxyphenylpyruvate | mutant M335H/P336A/P339T/T341D/E363Q/E426Q, pH 7,20°C | Arabidopsis thaliana | |
0.134 | - |
4-hydroxyphenylpyruvate | mutant M335H/P336A/E363Q, pH 7,20°C | Arabidopsis thaliana | |
0.32 | - |
4-hydroxyphenylpyruvate | pH 7,20°C | Zea mays |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Arabidopsis thaliana | P93836 | - |
- |
Zea mays | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-hydroxyphenylpyruvate + O2 | - |
Zea mays | homogentisate + CO2 | - |
? | |
4-hydroxyphenylpyruvate + O2 | - |
Arabidopsis thaliana | homogentisate + CO2 | - |
? |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.116 | - |
4-hydroxyphenylpyruvate | mutant M335H/P336A/P339T/T341D/E363Q/E426Q, pH 7,20°C | Arabidopsis thaliana | |
0.177 | - |
4-hydroxyphenylpyruvate | mutant M335H/P336A/E363Q, pH 7,20°C | Arabidopsis thaliana | |
0.183 | - |
4-hydroxyphenylpyruvate | wild-type, pH 7,20°C | Arabidopsis thaliana | |
0.51 | - |
4-hydroxyphenylpyruvate | pH 7,20°C | Zea mays |
IC50 Value | IC50 Value Maximum | Comment | Organism | Inhibitor | Structure |
---|---|---|---|---|---|
0.0000013 | - |
wild-type, pH 7, 20°C | Arabidopsis thaliana | topramezone | |
0.0000013 | - |
wild-type, pH 7, 20°C | Arabidopsis thaliana | isoxaflutole | |
0.0000016 | - |
mutant M335H/P336A/E363Q, pH 7, 20°C | Arabidopsis thaliana | topramezone | |
0.0000017 | - |
wild-type, pH 7, 20°C | Arabidopsis thaliana | sulcotrione | |
0.0000018 | - |
wild-type, pH 7, 20°C | Arabidopsis thaliana | mesotrione | |
0.0000022 | - |
wild-type, pH 7, 20°C | Arabidopsis thaliana | tembotrione | |
0.0000025 | - |
mutant M335H/P336A/E363Q, pH 7, 20°C | Arabidopsis thaliana | isoxaflutole | |
0.0000027 | - |
mutant M335H/P336A/E363Q, pH 7, 20°C | Arabidopsis thaliana | mesotrione | |
0.000004 | - |
mutant M335H/P336A/E363Q, pH 7, 20°C | Arabidopsis thaliana | tembotrione | |
0.0000041 | - |
mutant M335H/P336A/E363Q, pH 7, 20°C | Arabidopsis thaliana | sulcotrione | |
0.0000051 | - |
wild-type, pH 7, 20°C | Arabidopsis thaliana | bicyclopyrone | |
0.0000061 | - |
mutant M335H/P336A/P339T/T341D/E363Q/E426Q, pH 7, 20°C | Arabidopsis thaliana | topramezone | |
0.0000068 | - |
mutant M335H/P336A/P339T/T341D/E363Q/E426Q, pH 7, 20°C | Arabidopsis thaliana | mesotrione | |
0.0000072 | - |
pH 7, 20°C | Zea mays | topramezone | |
0.0000075 | - |
mutant M335H/P336A/P339T/T341D/E363Q/E426Q, pH 7, 20°C | Arabidopsis thaliana | isoxaflutole | |
0.0000087 | - |
pH 7, 20°C | Zea mays | tembotrione | |
0.00001 | - |
mutant M335H/P336A/P339T/T341D/E363Q/E426Q, pH 7, 20°C | Arabidopsis thaliana | tembotrione | |
0.000012 | - |
mutant M335H/P336A/P339T/T341D/E363Q/E426Q, pH 7, 20°C | Arabidopsis thaliana | sulcotrione | |
0.000015 | - |
mutant M335H/P336A/E363Q, pH 7, 20°C | Arabidopsis thaliana | bicyclopyrone | |
0.000023 | - |
pH 7, 20°C | Zea mays | bicyclopyrone | |
0.000042 | - |
pH 7, 20°C | Zea mays | isoxaflutole | |
0.000043 | - |
mutant M335H/P336A/P339T/T341D/E363Q/E426Q, pH 7, 20°C | Arabidopsis thaliana | bicyclopyrone | |
0.000062 | - |
pH 7, 20°C | Zea mays | mesotrione | |
0.000094 | - |
pH 7, 20°C | Zea mays | sulcotrione |