Cloned (Comment) | Organism |
---|---|
expression in Escherichia coli | Tupaia chinensis |
Protein Variants | Comment | Organism |
---|---|---|
V418I | mutant produces 21.5% of 12S- and 78.5% of 15S product | Tupaia chinensis |
V418I | mutant produces 23.1% of 12S- and 76.9% of 15S product | Tupaia chinensis |
V418I/419M | mutant produces 5.1% of 12S- and 94.9% of 15S product | Tupaia chinensis |
V418I/419M | mutant produces 5.9% of 12S- and 94.1% of 15S product | Tupaia chinensis |
V418I/419M/353A | mutant produces 89.6% of 12S- and 10.2% of 15S product | Tupaia chinensis |
V418I/419M/353A | mutant produces 92.9% of 12S- and 7.1% of 15S product | Tupaia chinensis |
V418I/419M/353L | mutant produces 16.9% of 12S- and 83.1% of 15S product | Tupaia chinensis |
V418I/419M/353L | mutant produces 77.6% of 12S- and 22.4% of 15S product | Tupaia chinensis |
V419M | mutant produces 5.46% of 12S- and 45.5% of 15S product | Tupaia chinensis |
V419M | mutant produces 75.9% of 12S- and 24.1% of 15S product | Tupaia chinensis |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0185 | - |
O2 | pH 7.4, temperature not specified in the publication | Tupaia chinensis | |
0.0874 | - |
O2 | pH 7.4, temperature not specified in the publication | Tupaia chinensis | |
0.116 | - |
arachidonate | pH 7.4, temperature not specified in the publication | Tupaia chinensis | |
0.232 | - |
arachidonate | pH 7.4, temperature not specified in the publication | Tupaia chinensis |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Tupaia chinensis | A0A8A0R1W6 | isoform Alox15a | - |
Tupaia chinensis | A0A8A0R235 | isoform Alox15c | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
arachidonate + O2 | - |
Tupaia chinensis | (12S)-hydroperoxy-(5Z,8Z,10E,14Z)-eicosatetraenoic acid | - |
? | |
additional information | enzyme additionally shows membrane oxygenase activity | Tupaia chinensis | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
? | x * 75000, SDS-PAGE, x * 75155, calculated from sequence | Tupaia chinensis |
? | x * 75000, SDS-PAGE, x * 75205, calculated from sequence | Tupaia chinensis |
Synonyms | Comment | Organism |
---|---|---|
Alox15 | - |
Tupaia chinensis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | - |
- |
Tupaia chinensis |
25 | - |
- |
Tupaia chinensis |