Cloned (Comment) | Organism |
---|---|
the recombinant wild-type enzyme and various mutants are expressed in Escherichia coli as His-tagged fusion proteins | Oryctolagus cuniculus |
Protein Variants | Comment | Organism |
---|---|---|
F353L | the ratio of turnover-number to KM-value is 4fold lower than the value for the wild-type enzyme. The mutation strongly alters the positional specificity of the oxygenation of most substrates in favor of 12-lipoxygenation except from (16(S),5Z,8Z,11Z,14Z)-16-hydroxyeicosa-5,8,11,14-tetraenoic acid | Oryctolagus cuniculus |
I593A | mutation induces alterations in the stereochemical characteristics of hydroxy fatty acid oxygenation | Oryctolagus cuniculus |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.00301 | - |
arachidonic acid | pH 7.4, wild-type enzyme | Oryctolagus cuniculus | |
0.0087 | - |
arachidonic acid | pH 7.4, mutant enzyme F353L | Oryctolagus cuniculus | |
0.0227 | - |
(16(S),5Z,8Z,11Z,14Z)-16-hydroxyeicosa-5,8,11,14-tetraenoic acid | pH 7.4, mutant enzyme F353L | Oryctolagus cuniculus | |
0.0236 | - |
(16(S),5Z,8Z,11Z,14Z)-16-hydroxyeicosa-5,8,11,14-tetraenoic acid | pH 7.4, wild-type enzyme | Oryctolagus cuniculus | |
0.0289 | - |
(16(R),5Z,8Z,11Z,14Z)-16-hydroxyeicosa-5,8,11,14-tetraenoic acid | pH 7.4, wild-type enzyme | Oryctolagus cuniculus | |
0.0313 | - |
(17(S),5Z,8Z,11Z,14Z)-17-hydroxyeicosa-5,8,11,14-tetraenoic acid | pH 7.4, mutant enzyme L353L | Oryctolagus cuniculus | |
0.0368 | - |
(17(R),5Z,8Z,11Z,14Z)-17-hydroxyeicosa-5,8,11,14-tetraenoic acid | pH 7.4, mutant enzyme L353L | Oryctolagus cuniculus | |
0.136 | - |
(16(R),5Z,8Z,11Z,14Z)-16-hydroxyeicosa-5,8,11,14-tetraenoic acid | pH 7.4, mutant enzyme F353L | Oryctolagus cuniculus | |
0.236 | - |
(17(S),5Z,8Z,11Z,14Z)-17-hydroxyeicosa-5,8,11,14-tetraenoic acid | pH 7.4, wild-type enzyme | Oryctolagus cuniculus | |
0.293 | - |
(17(R),5Z,8Z,11Z,14Z)-17-hydroxyeicosa-5,8,11,14-tetraenoic acid | pH 7.4, wild-type enzyme | Oryctolagus cuniculus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Oryctolagus cuniculus | P12530 | - |
- |
Purification (Comment) | Organism |
---|---|
- |
Oryctolagus cuniculus |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
additional information | stroma-free hemolysis supernatant of a reticulocyte-rich blood cell suspension | Oryctolagus cuniculus | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
(16(R),5Z,8Z,11Z,14Z)-16-fluoroeicosa-5,8,11,14-tetraenoic acid + O2 | - |
Oryctolagus cuniculus | ? | wild-type enzyme: 78% of the 15,16(R) product and 22% of the 12,16(R) product | ? | |
(16(R),5Z,8Z,11Z,14Z)-16-hydroxyeicosa-5,8,11,14-tetraenoic acid + O2 | - |
Oryctolagus cuniculus | ? | wild-type enzyme and mutant enzyme I593A prouce small amounts of unspecific products. Mutant enzyme F353L produces 6% of 15,16(R) product and 94% of the 12,16(R) product | ? | |
(16(S),5Z,8Z,11Z,14Z)-16-fluoroeicosa-5,8,11,14-tetraenoic acid + O2 | - |
Oryctolagus cuniculus | ? | wild-type enzyme: 69% of the 15,16(S) product and 31% of the 12,16(S) product | ? | |
(16(S)5Z,8Z,11Z,14Z)-16-hydroxyeicosa-5,8,11,14-tetraenoic acid + O2 | - |
Oryctolagus cuniculus | ? | wild-type enzyme: 93% of the 15,16(S) product and 7% of the 12,16(S) product | ? | |
(17(R),5Z,8Z,11Z,14Z)-17-hydroxyeicosa-5,8,11,14-tetraenoic acid + O2 | - |
Oryctolagus cuniculus | ? | wild-type enzyme: 1% of the 15,17(R) product and 99% of the 12,17(R) product | ? | |
(17(S),5Z,8Z,11Z,14Z)-17-hydroxyeicosa-5,8,11,14-tetraenoic acid + O2 | - |
Oryctolagus cuniculus | ? | wild-type enzyme: 3% of the 15,17(S) product and 97% of the 12,17(S) product | ? | |
(18(R),5Z,8Z,11Z,14Z)-18-hydroxyeicosa-5,8,11,14-tetraenoic acid + O2 | oxygenation proceeds with little if any enantioselectivity | Oryctolagus cuniculus | ? | - |
? | |
(18(S),5Z,8Z,11Z,14Z)-18-hydroxyeicosa-5,8,11,14-tetraenoic acid + O2 | oxygenation proceeds with little if any enantioselectivity | Oryctolagus cuniculus | ? | - |
? | |
arachidonic acid + O2 | - |
Oryctolagus cuniculus | (15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoic acid + (12S,5Z,8Z,10E,14Z)-12-hydroperoxyeicosa-5,8,10,14-tetraenoic acid | 93% (15S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoic acid and 7% (12S)-hydroperoxy-(5Z,8Z,11Z,13E)-eicosatetraenoic acid | ? |
Synonyms | Comment | Organism |
---|---|---|
15-lipoxygenase 1 | - |
Oryctolagus cuniculus |
15-LOX | - |
Oryctolagus cuniculus |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.08 | - |
(16(R),5Z,8Z,11Z,14Z)-16-hydroxyeicosa-5,8,11,14-tetraenoic acid | pH 7.4, mutant enzyme F353L | Oryctolagus cuniculus | |
0.18 | - |
(17(R),5Z,8Z,11Z,14Z)-17-hydroxyeicosa-5,8,11,14-tetraenoic acid | pH 7.4, wild-type enzyme | Oryctolagus cuniculus | |
0.2 | - |
(16(S),5Z,8Z,11Z,14Z)-16-hydroxyeicosa-5,8,11,14-tetraenoic acid | pH 7.4, mutant enzyme F353L | Oryctolagus cuniculus | |
0.2 | - |
(17(R),5Z,8Z,11Z,14Z)-17-hydroxyeicosa-5,8,11,14-tetraenoic acid | pH 7.4, mutant enzyme L353L | Oryctolagus cuniculus | |
0.24 | - |
(17(S),5Z,8Z,11Z,14Z)-17-hydroxyeicosa-5,8,11,14-tetraenoic acid | pH 7.4, mutant enzyme L353L | Oryctolagus cuniculus | |
0.51 | - |
(16(S),5Z,8Z,11Z,14Z)-16-hydroxyeicosa-5,8,11,14-tetraenoic acid | pH 7.4, wild-type enzyme | Oryctolagus cuniculus | |
1.24 | - |
(17(S),5Z,8Z,11Z,14Z)-17-hydroxyeicosa-5,8,11,14-tetraenoic acid | pH 7.4, wild-type enzyme | Oryctolagus cuniculus | |
6.02 | - |
arachidonic acid | pH 7.4, mutant enzyme F353L | Oryctolagus cuniculus | |
8.16 | - |
arachidonic acid | pH 7.4, wild-type enzyme | Oryctolagus cuniculus |