Cloned (Comment) | Organism |
---|---|
gene bphC_meta, DNA and amino acid sequence determination and analysis, expression in Escherichia coli | uncultured bacterium |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ba2+ | 1 mM, moderate inhibition | uncultured bacterium | |
Ca2+ | 1 mM, slight inhibition with substrate biphenyl-2,3-diol, moderate with catechol, but activating with 3-methylcatechol | uncultured bacterium | |
Co2+ | 1 mM, complete inhibition | uncultured bacterium | |
Cu2+ | 1 mM, complete inhibition | uncultured bacterium | |
Hg2+ | 1 mM, complete inhibition | uncultured bacterium | |
Mg2+ | 1 mM, moderate inhibition | uncultured bacterium | |
Mn2+ | 1 mM, moderate inhibition | uncultured bacterium | |
Ni2+ | 1 mM, strong inhibition | uncultured bacterium | |
Zn2+ | 1 mM, complete inhibition with substrate biphenyl-2,3-diol, not with catechols | uncultured bacterium |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0067 | - |
biphenyl-2,3-diol | pH 9.0, 40°C, recombinant enzyme | uncultured bacterium | |
0.49 | - |
3-methylcatechol | pH 9.0, 40°C, recombinant enzyme | uncultured bacterium | |
0.606 | - |
catechol | pH 9.0, 40°C, recombinant enzyme | uncultured bacterium |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Ca2+ | 1 mM, slight inhibition with substrate biphenyl-2,3-diol, moderate with catechol, but activating with 3-methylcatechol | uncultured bacterium | |
Fe3+ | 1 mM, activates | uncultured bacterium |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
32000 | - |
x * 32000, recombinant enzyme, SDS-PAGE | uncultured bacterium |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
biphenyl-2,3-diol + O2 | uncultured bacterium | - |
2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
uncultured bacterium | F2X5L9 | activated sludge metagenome, gene bphC_meta | - |
Purification (Comment) | Organism |
---|---|
recombinant enzyme from Escherichia coli | uncultured bacterium |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-methylcatechol + O2 | - |
uncultured bacterium | ? | - |
? | |
biphenyl-2,3-diol + O2 | - |
uncultured bacterium | 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate | - |
? | |
biphenyl-2,3-diol + O2 | preferred substrate | uncultured bacterium | 2-hydroxy-6-oxo-6-phenylhexa-2,4-dienoate | - |
? | |
catechol + O2 | - |
uncultured bacterium | cis,cis-muconate | - |
? | |
additional information | activity of recombinant enzyme toward substituted catechols is in descending order: 2,3-dihydroxybiphenyl, 3-methylcatechol, catechol, and 4-chlorocatechol/4-methylcatechol, substrate binding analysis by molecular docking | uncultured bacterium | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 32000, recombinant enzyme, SDS-PAGE | uncultured bacterium |
More | enzyme homology modeling, overview | uncultured bacterium |
Synonyms | Comment | Organism |
---|---|---|
2,3-dihydroxybiphenyl 1,2-dioxygenase | - |
uncultured bacterium |
BphC | - |
uncultured bacterium |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
recombinant enzyme | uncultured bacterium |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
38 | - |
catechol | pH 9.0, 40°C, recombinant enzyme | uncultured bacterium | |
44 | - |
3-methylcatechol | pH 9.0, 40°C, recombinant enzyme | uncultured bacterium | |
165 | - |
biphenyl-2,3-diol | pH 9.0, 40°C, recombinant enzyme | uncultured bacterium |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
9 | - |
recombinant enzyme | uncultured bacterium |
General Information | Comment | Organism |
---|---|---|
metabolism | BphC is the key enzyme for polychlorobiphenyl transformation | uncultured bacterium |
additional information | enzyme homology modeling, overview | uncultured bacterium |
physiological function | BphC catalyzes the ring cleavage of 2,3-dihydroxybiphenyl during biphenyl/polychlorobipheny degradation process | uncultured bacterium |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
62.7 | - |
catechol | pH 9.0, 40°C, recombinant enzyme | uncultured bacterium | |
89.8 | - |
3-methylcatechol | pH 9.0, 40°C, recombinant enzyme | uncultured bacterium | |
24630 | - |
biphenyl-2,3-diol | pH 9.0, 40°C, recombinant enzyme | uncultured bacterium |