Cloned (Comment) | Organism |
---|---|
overexpression of His6-tagged enzyme in Escherichia coli BL21(DE3) | Rhizorhabdus dicambivorans |
General Stability | Organism |
---|---|
the enzyme is very unstable and could not be purified in the absence of Fe2+ | Rhizorhabdus dicambivorans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Ag+ | 1 mM, severely inhibited | Rhizorhabdus dicambivorans | |
Al3+ | 1 mM, moderately inhibited | Rhizorhabdus dicambivorans | |
Cd2+ | 1 mM, severely inhibited | Rhizorhabdus dicambivorans | |
Co2+ | 1 mM, moderately inhibited | Rhizorhabdus dicambivorans | |
EDTA | 5 mM, moderately inhibited | Rhizorhabdus dicambivorans | |
Hg2+ | 1 mM, severely inhibited | Rhizorhabdus dicambivorans | |
Mn2+ | 1 mM, moderately inhibited | Rhizorhabdus dicambivorans | |
Ni2+ | 1 mM, moderately inhibited | Rhizorhabdus dicambivorans | |
Zn2+ | 1 mM, severely inhibited | Rhizorhabdus dicambivorans |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0181 | - |
2,5-Dihydroxybenzoate | pH 7.4, 30°C | Rhizorhabdus dicambivorans | |
0.036 | - |
3-Chlorogentisate | pH 7.4, 30°C | Rhizorhabdus dicambivorans |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | 1 mM, enhances activity | Rhizorhabdus dicambivorans | |
Fe3+ | 1 mM, enhances activity | Rhizorhabdus dicambivorans | |
Na+ | 1 mM, enhances activity | Rhizorhabdus dicambivorans |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
70000 | - |
gel filtration | Rhizorhabdus dicambivorans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,5-dihydroxybenzoate + O2 | Rhizorhabdus dicambivorans | - |
maleylpyruvate | - |
? | |
2,5-dihydroxybenzoate + O2 | Rhizorhabdus dicambivorans Ndbn-20 | - |
maleylpyruvate | - |
? | |
3-chlorogentisate + O2 | Rhizorhabdus dicambivorans | - |
3-chloro-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid | - |
? | |
3-chlorogentisate + O2 | Rhizorhabdus dicambivorans Ndbn-20 | - |
3-chloro-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Rhizorhabdus dicambivorans | - |
- |
- |
Rhizorhabdus dicambivorans Ndbn-20 | - |
- |
- |
Purification (Comment) | Organism |
---|---|
- |
Rhizorhabdus dicambivorans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
0.6 | - |
substrate: 2,5-dihydroxybenzoate, pH 7.4, 30°C | Rhizorhabdus dicambivorans |
15.4 | - |
substrate: 3-chlorogentisate, pH 7.4, 30°C | Rhizorhabdus dicambivorans |
Storage Stability | Organism |
---|---|
-80°C, 2 weeks, purified enzyme loses 50% of its initial activity. Addition of 2.0 mM GSH (or cysteine), 0.1 mM Fe(NH4)2(SO4)2*6H2O, and 10% glycerol stabilizes the activity | Rhizorhabdus dicambivorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,5-dihydroxybenzoate + O2 | - |
Rhizorhabdus dicambivorans | maleylpyruvate | - |
? | |
2,5-dihydroxybenzoate + O2 | - |
Rhizorhabdus dicambivorans Ndbn-20 | maleylpyruvate | - |
? | |
3-chlorogentisate + O2 | - |
Rhizorhabdus dicambivorans | 3-chloro-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid | - |
? | |
3-chlorogentisate + O2 | - |
Rhizorhabdus dicambivorans Ndbn-20 | 3-chloro-4-hydroxy-6-oxo-hepta-2,4-dienedioic acid | - |
? | |
additional information | no activity with 3,6-dichlorogentisate or 6-chlorogentisate | Rhizorhabdus dicambivorans | ? | - |
- |
|
additional information | no activity with 3,6-dichlorogentisate or 6-chlorogentisate | Rhizorhabdus dicambivorans Ndbn-20 | ? | - |
- |
Subunits | Comment | Organism |
---|---|---|
dimer | 2 * 39700, SDS-PAGE | Rhizorhabdus dicambivorans |
Synonyms | Comment | Organism |
---|---|---|
DsmD | - |
Rhizorhabdus dicambivorans |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
in 50 mM Tris-HCl | Rhizorhabdus dicambivorans |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.37 | - |
2,5-Dihydroxybenzoate | pH 7.4, 30°C | Rhizorhabdus dicambivorans | |
10.33 | - |
3-Chlorogentisate | pH 7.4, 30°C | Rhizorhabdus dicambivorans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.4 | - |
- |
Rhizorhabdus dicambivorans |
Organism | Comment | Expression |
---|---|---|
Rhizorhabdus dicambivorans | transcription of dsmD is induced by 3-chlorogentisate but not gentisate | up |
General Information | Comment | Organism |
---|---|---|
malfunction | disruption of dsmD results in a significant decline in the degradation rates of 3-chlorogentisate and the herbicide dicamba but has no effect on the degradation of gentisate. The disruption of both dsmD and gtdA leads to the inability to degrade 3-chlorogentisate and gentisate | Rhizorhabdus dicambivorans |
physiological function | DsmD is specifically responsible for cleavage of 3-chlorogentisate, a key intermediate in the catabolism of the herbicide dicamba in Rhizorhabdus dicambivorans Ndbn-20 | Rhizorhabdus dicambivorans |
kcat/KM Value [1/mMs-1] | kcat/KM Value Maximum [1/mMs-1] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
20.44 | - |
2,5-Dihydroxybenzoate | pH 7.4, 30°C | Rhizorhabdus dicambivorans | |
286.1 | - |
3-Chlorogentisate | pH 7.4, 30°C | Rhizorhabdus dicambivorans |