Application | Comment | Organism |
---|---|---|
medicine | tryptophan 2,3-dioxygenase is considered as a drug target for cancer immunotherapy | Drosophila melanogaster |
Cloned (Comment) | Organism |
---|---|
expression of His6-tagged TDO in Escherichia coli strain BL21 (DE3) | Drosophila melanogaster |
Crystallization (Comment) | Organism |
---|---|
purified His6-tagged recombinant enzyme in complex with heme, hanging drop vapor diffusion method, mixing of 20 mg/ml protein in 20 mM Tris, pH 8.0, and 200 mM NaCl, with reservoir solution containing 0.1 M HEPES, pH 7.0, and 1.1 M sodium malonate, at equal volumes, 20°C, X-ray diffraction structure determination and analysis at 2.7 A resolution | Drosophila melanogaster |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | heme-scontaining enzyme | Drosophila melanogaster |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tryptophan + O2 | Drosophila melanogaster | - |
N-formyl-L-kynurenine | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Drosophila melanogaster | P20351 | - |
- |
Purification (Comment) | Organism |
---|---|
recombinant His6-tagged TDO from Escherichia coli strain BL21 (DE3) by nickel affinity chromatography and gel filtration to homogeneity | Drosophila melanogaster |
Reaction | Comment | Organism | Reaction ID |
---|---|---|---|
L-tryptophan + O2 = N-formyl-L-kynurenine | substrate recognition and catalytic mechanism, overview | Drosophila melanogaster |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
L-tryptophan + O2 | - |
Drosophila melanogaster | N-formyl-L-kynurenine | - |
? | |
L-tryptophan + O2 | enzyme shows induced-fit mechanism to bind L-Trp, two conserved but flexible loops undergo conformational changes, converting the active site from an open conformation to a closed conformation, key residues involved in recognition and binding of the heme and the substrate, Molecular modeling and dynamics simulation, overview | Drosophila melanogaster | N-formyl-L-kynurenine | - |
? |
Subunits | Comment | Organism |
---|---|---|
tetramer | the enzme consists of an N-terminal segment, a large domain and a small domain, and assumes a tetrameric architecture. DmTDO contains two major insertion sequences: one forms part of the heme-binding site and the other forms a large portion of the small domain | Drosophila melanogaster |
Synonyms | Comment | Organism |
---|---|---|
TDO | - |
Drosophila melanogaster |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
heme | DmTDO contains two major insertion sequences: one forms part of the heme-binding site and the other forms a large portion of the small domain, bindig structure, overview | Drosophila melanogaster |
General Information | Comment | Organism |
---|---|---|
metabolism | tryptophan 2,3-dioxygenase catalyzes the oxidative cleavage of the indole ring of L-tryptophan to N-formylkynurenine in the kynurenine pathway | Drosophila melanogaster |