BRENDA - Enzyme Database show
show all sequences of 1.13.11.6

Crystal structure of 3-hydroxyanthranilic acid 3,4-dioxygenase from Saccharomyces cerevisiae: a special subgroup of the type III extradiol dioxygenases

Li, X.; Guo, M.; Fan, J.; Tang, W.; Wang, D.; Ge, H.; Rong, H.; Teng, M.; Niu, L.; Liu, Q.; Hao, Q.; Protein Sci. 15, 761-773 (2006)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
Y3HAO protein is subcloned into the pET-28a expression vector from extracted Saccharomyces cerevisiae genomic DNA, and the Y3HAO protein is highly expressed as a soluble protein in Escherichia coli strain BL21(DE3) with a six-residueHis tag attached to its N-terminus
Saccharomyces cerevisiae
Crystallization (Commentary)
Crystallization
Organism
hanging drop-vapor diffusion method, 2.4 A resolution
Saccharomyces cerevisiae
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
non-heme ferrous enzyme
Saccharomyces cerevisiae
Ni2+
two nickel binding sites per molecule. One of the bound nickel atoms occupies the proposed ferrous-coordinated active site
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3-hydroxyanthranilate + O2
Saccharomyces cerevisiae
in the kynurenine pathway
2-amino-3-carboxymuconate semialdehyde
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Saccharomyces cerevisiae
-
-
-
Purification (Commentary)
Commentary
Organism
recombinant
Saccharomyces cerevisiae
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-hydroxyanthranilate + O2
in the kynurenine pathway
677020
Saccharomyces cerevisiae
2-amino-3-carboxymuconate semialdehyde
-
-
-
?
Cofactor
Cofactor
Commentary
Organism
Structure
additional information
non-heme enzyme
Saccharomyces cerevisiae
Cloned(Commentary) (protein specific)
Commentary
Organism
Y3HAO protein is subcloned into the pET-28a expression vector from extracted Saccharomyces cerevisiae genomic DNA, and the Y3HAO protein is highly expressed as a soluble protein in Escherichia coli strain BL21(DE3) with a six-residueHis tag attached to its N-terminus
Saccharomyces cerevisiae
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
additional information
non-heme enzyme
Saccharomyces cerevisiae
Crystallization (Commentary) (protein specific)
Crystallization
Organism
hanging drop-vapor diffusion method, 2.4 A resolution
Saccharomyces cerevisiae
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
non-heme ferrous enzyme
Saccharomyces cerevisiae
Ni2+
two nickel binding sites per molecule. One of the bound nickel atoms occupies the proposed ferrous-coordinated active site
Saccharomyces cerevisiae
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3-hydroxyanthranilate + O2
Saccharomyces cerevisiae
in the kynurenine pathway
2-amino-3-carboxymuconate semialdehyde
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
recombinant
Saccharomyces cerevisiae
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
3-hydroxyanthranilate + O2
in the kynurenine pathway
677020
Saccharomyces cerevisiae
2-amino-3-carboxymuconate semialdehyde
-
-
-
?
Other publictions for EC 1.13.11.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
723950
Chakraborty
Aromatic ring cleavage of 2-am ...
Pseudomonas pseudoalcaligenes
Angew. Chem. Int. Ed. Engl.
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920-924
2013
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3
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Dilovic
Crystal structure of bovine 3- ...
Bos taurus
Biopolymers
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4-chloro-3-hydroxyanthranilate ...
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Acetylsalicylic acid and aceta ...
Rattus norvegicus, Rattus norvegicus Wistar
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677020
Li
Crystal structure of 3-hydroxy ...
Saccharomyces cerevisiae
Protein Sci.
15
761-773
2006
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672036
Colabroy
The mechanism of inactivation ...
Cupriavidus metallidurans
Biochemistry
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672037
Zhang
Structural studies on 3-hydrox ...
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Biochemistry
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2005
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3
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673130
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Prokaryotic homologs of the eu ...
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13
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1
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658927
Nandi
Purification and inactivation ...
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3
2
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1
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439377
Calderone
Cloning of human 3-hydroxyanth ...
Homo sapiens
Biochim. Biophys. Acta
1596
283-292
2002
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439378
Agrawal
QSAR study on inhibition of br ...
Rattus norvegicus
Bioorg. Med. Chem.
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3295-3299
2001
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439373
Fornstedt-Wallin
3-Hydroxyanthranilic acid accu ...
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Eur. J. Pharmacol.
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1999
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Effects of mitochondria and o- ...
Mus musculus
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Molecular characterization of ...
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439374
Roberts
3-Hydroxyanthranilic acid oxyg ...
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439372
Roberts
Immunocytochemical localizatio ...
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439368
Walsh
4-Halo-3-hydroxyanthranilic ac ...
Rattus norvegicus
Biochem. Pharmacol.
42
985-990
1991
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3
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1
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3
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439369
Serrano
Liver 3-hydroxyanthranilic aci ...
Oncorhynchus mykiss
Comp. Biochem. Physiol. B
99
275-280
1991
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2
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4
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439367
Okuno
Rat 3-hydroxyanthranilic acid ...
Rattus norvegicus
J. Neurochem.
49
771-780
1987
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1
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1
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1
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4
3
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1
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1
1
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4
3
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2
1
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1
1
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439366
Foster
Synthesis of quinolinic acid b ...
Rattus norvegicus
J. Neurochem.
47
23-30
1986
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1
1
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1
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2
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1
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439365
Koontz
Beef kidney 3-hydroxyanthranil ...
Bos taurus
J. Biol. Chem.
251
368-377
1976
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439370
Savage
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The purification and some prop ...
Papio ursinus
Int. J. Biochem.
6
459-466
1975
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439364
Decker
Purification and properties of ...
Bos taurus
J. Biol. Chem.
236
3076-3082
1961
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