BRENDA - Enzyme Database show
show all sequences of 1.13.11.6

Crystal structure of bovine 3-hydroxyanthranilate 3,4-dioxygenase

Dilovic, I.; Gliubich, F.; Malpeli, G.; Zanotti, G.; Matkovic-Calogovic, D.; Biopolymers 91, 1189-1195 (2009)

Data extracted from this reference:

Crystallization (Commentary)
Crystallization
Organism
first vapor diffusion with sitting drop method starting from protein concentration of 10 mg/ml in 32% (NH4)2SO4, 0.1 M sodium acetate, 10 mM 2-mercaptoethanol, pH 5, subsequently, crystals can be obtained by seeding starting from fragments of first crystallization experiments using 40% (NH4)2SO4, Tris-HCl, 40 mM MgCl2, 3% MPD, pH 8 as precipitant
Bos taurus
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
33000
-
SDS-PAGE, nondenaturating PAGE resolves two components, purified by FPLC on protein PakGlass DEAE-4 PW column, one component is N-terminally truncated annexin IV
Bos taurus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-amino-3-hydroxybenzoic acid + O2
Bos taurus
-
2,3-pyridinedicarboxylic acid + H2O
intermediate 2-amino-3-carboxymuconic acid semialdehyde
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Bos taurus
Q0VCA8
-
-
Purification (Commentary)
Commentary
Organism
bovine kidney homogenized in 0.1 M potassium dihydrogen phosphate buffer with 20% glycerol and 3 mM 2-mercaptoethanol, pH 7.4, centrifuged, protein fraction of supernatant that precipitates from 34-62% saturated (NH4)2SO4 is resuspended in 5 mM potassium dihydrogen phosphate buffer with 20% glycerol and 3 mM 2-mercaptoethanol, pH 7.4, dialyzed against same buffer containing protease inhibitor PMSF (0.1 mM), applied to DEAE Sephadex A-50 column, fractions with enzyme activity pooled and concentrated by precipitation with 80%-saturated (NH4)2SO4, dialyzed against 10 mM Tris-HCl with 20% glycerol and 3 mM 2-mercaptoethanol, pH 7.4, applied to Blue-Sepharose CL-4B column, concentration of active fractions by ultrafiltration through YM-10 membrane
Bos taurus
Source Tissue
Source Tissue
Commentary
Organism
Textmining
kidney
-
Bos taurus
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-amino-3-hydroxybenzoic acid + O2
-
696784
Bos taurus
2,3-pyridinedicarboxylic acid + H2O
intermediate 2-amino-3-carboxymuconic acid semialdehyde
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 33000, SDS-PAGE, 286 amino acids, 2 domains that represent the dimers of the prokaryote enzyme structure which is also conserved in simple eukaryotes
Bos taurus
Crystallization (Commentary) (protein specific)
Crystallization
Organism
first vapor diffusion with sitting drop method starting from protein concentration of 10 mg/ml in 32% (NH4)2SO4, 0.1 M sodium acetate, 10 mM 2-mercaptoethanol, pH 5, subsequently, crystals can be obtained by seeding starting from fragments of first crystallization experiments using 40% (NH4)2SO4, Tris-HCl, 40 mM MgCl2, 3% MPD, pH 8 as precipitant
Bos taurus
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
33000
-
SDS-PAGE, nondenaturating PAGE resolves two components, purified by FPLC on protein PakGlass DEAE-4 PW column, one component is N-terminally truncated annexin IV
Bos taurus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
2-amino-3-hydroxybenzoic acid + O2
Bos taurus
-
2,3-pyridinedicarboxylic acid + H2O
intermediate 2-amino-3-carboxymuconic acid semialdehyde
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
bovine kidney homogenized in 0.1 M potassium dihydrogen phosphate buffer with 20% glycerol and 3 mM 2-mercaptoethanol, pH 7.4, centrifuged, protein fraction of supernatant that precipitates from 34-62% saturated (NH4)2SO4 is resuspended in 5 mM potassium dihydrogen phosphate buffer with 20% glycerol and 3 mM 2-mercaptoethanol, pH 7.4, dialyzed against same buffer containing protease inhibitor PMSF (0.1 mM), applied to DEAE Sephadex A-50 column, fractions with enzyme activity pooled and concentrated by precipitation with 80%-saturated (NH4)2SO4, dialyzed against 10 mM Tris-HCl with 20% glycerol and 3 mM 2-mercaptoethanol, pH 7.4, applied to Blue-Sepharose CL-4B column, concentration of active fractions by ultrafiltration through YM-10 membrane
Bos taurus
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
kidney
-
Bos taurus
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2-amino-3-hydroxybenzoic acid + O2
-
696784
Bos taurus
2,3-pyridinedicarboxylic acid + H2O
intermediate 2-amino-3-carboxymuconic acid semialdehyde
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 33000, SDS-PAGE, 286 amino acids, 2 domains that represent the dimers of the prokaryote enzyme structure which is also conserved in simple eukaryotes
Bos taurus
Other publictions for EC 1.13.11.6
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741516
Pidugu
Crystal structures of human 3 ...
Homo sapiens
Acta Crystallogr. Sect. D
73
340-348
2017
-
2
1
1
-
-
1
-
-
2
-
1
-
2
-
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
2
1
-
1
-
-
-
1
-
-
-
2
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
743276
Brkic
Human 3-hydroxyanthranilate 3 ...
Homo sapiens
Mol. Biosyst.
11
898-907
2015
-
1
-
-
3
-
-
-
-
1
-
1
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
3
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
723950
Chakraborty
Aromatic ring cleavage of 2-am ...
Pseudomonas pseudoalcaligenes
Angew. Chem. Int. Ed. Engl.
52
920-924
2013
-
-
-
-
-
-
-
-
-
1
-
1
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
696784
Dilovic
Crystal structure of bovine 3- ...
Bos taurus
Biopolymers
91
1189-1195
2009
-
-
-
1
-
-
-
-
-
-
1
1
-
4
-
-
1
-
-
2
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
1
-
2
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
675557
Yates Jennifer
4-chloro-3-hydroxyanthranilate ...
Cavia porcellus, Cavia porcellus Hartley
J. Neurotrauma
23
866-881
2006
-
-
-
-
-
-
1
-
-
-
-
-
-
11
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
675782
Maharaj
Acetylsalicylic acid and aceta ...
Rattus norvegicus, Rattus norvegicus Wistar
Metab. Brain Dis.
21
189-199
2006
-
-
-
-
-
-
2
-
-
-
-
-
-
168
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
677020
Li
Crystal structure of 3-hydroxy ...
Saccharomyces cerevisiae
Protein Sci.
15
761-773
2006
-
-
1
1
-
-
-
-
-
2
-
1
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
1
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
672036
Colabroy
The mechanism of inactivation ...
Cupriavidus metallidurans
Biochemistry
44
7623-7631
2005
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
672037
Zhang
Structural studies on 3-hydrox ...
Cupriavidus metallidurans
Biochemistry
44
7632-7643
2005
-
-
-
1
3
-
1
4
-
1
-
-
-
1
-
-
1
-
-
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
1
3
-
-
1
-
4
-
1
-
-
-
-
-
1
-
-
-
-
1
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
673130
Comai
The effect of age on the enzym ...
Rattus norvegicus
Clin. Chim. Acta
360
67-80
2005
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
649382
Muraki
Prokaryotic homologs of the eu ...
Pseudomonas fluorescens, Pseudomonas fluorescens KU-7
Appl. Environ. Microbiol.
69
1564-1572
2003
-
-
1
-
3
-
13
-
-
1
2
2
-
5
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
3
-
-
13
-
-
-
1
2
2
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
658927
Nandi
Purification and inactivation ...
Bos taurus
Int. J. Biochem. Cell Biol.
35
1085-1097
2003
-
-
-
-
-
-
3
2
-
-
4
1
-
2
-
-
1
-
-
2
1
-
2
1
-
-
1
-
1
-
-
-
5
2
-
-
-
-
-
-
-
-
-
3
5
2
-
-
4
1
-
-
-
1
-
2
1
-
2
1
-
-
1
-
1
-
-
2
-
-
-
-
-
-
439377
Calderone
Cloning of human 3-hydroxyanth ...
Homo sapiens
Biochim. Biophys. Acta
1596
283-292
2002
-
-
1
-
-
-
3
-
1
1
1
1
-
3
-
-
-
1
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
3
-
-
1
1
1
1
-
-
-
-
-
-
-
-
2
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
439378
Agrawal
QSAR study on inhibition of br ...
Rattus norvegicus
Bioorg. Med. Chem.
9
3295-3299
2001
-
-
-
-
-
-
1
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439373
Fornstedt-Wallin
3-Hydroxyanthranilic acid accu ...
Rattus norvegicus
Eur. J. Pharmacol.
386
15-24
1999
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
3
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439376
Chiarugi
Effects of mitochondria and o- ...
Mus musculus
J. Neurochem.
72
1125-1132
1999
-
-
-
-
-
-
1
-
1
-
1
-
-
1
-
-
1
-
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
1
-
-
-
-
1
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439379
Kucharczyk
The yeast gene YJR025c encodes ...
Escherichia coli, Saccharomyces cerevisiae
FEBS Lett.
424
127-130
1998
-
-
2
-
-
-
-
2
-
-
-
-
-
4
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439375
Cesura
Molecular characterization of ...
Rattus norvegicus
Adv. Exp. Med. Biol.
398
477-483
1996
-
-
1
-
-
-
1
1
-
-
2
-
-
1
-
-
-
-
-
-
-
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
1
-
-
2
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
439374
Roberts
3-Hydroxyanthranilic acid oxyg ...
Rattus norvegicus
J. Neurosci.
15
1150-1161
1995
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439371
Malherbe
Molecular cloning and function ...
Rattus norvegicus
J. Biol. Chem.
269
13792-13797
1994
-
1
1
-
-
-
-
-
-
-
1
-
-
3
-
-
1
-
-
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
1
-
1
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439372
Roberts
Immunocytochemical localizatio ...
Rattus norvegicus
Brain Res.
650
229-238
1994
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439368
Walsh
4-Halo-3-hydroxyanthranilic ac ...
Rattus norvegicus
Biochem. Pharmacol.
42
985-990
1991
-
-
-
-
-
-
3
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
3
3
-
-
-
-
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439369
Serrano
Liver 3-hydroxyanthranilic aci ...
Oncorhynchus mykiss
Comp. Biochem. Physiol. B
99
275-280
1991
-
-
-
-
-
2
-
1
-
-
-
-
-
4
-
-
1
-
-
2
-
2
1
-
-
1
-
-
1
1
1
-
-
-
-
-
-
-
-
-
-
2
-
-
-
1
-
-
-
-
-
-
-
1
-
2
-
2
1
-
-
1
-
-
1
1
1
-
-
-
-
-
-
-
439367
Okuno
Rat 3-hydroxyanthranilic acid ...
Rattus norvegicus
J. Neurochem.
49
771-780
1987
-
-
-
-
-
-
-
1
-
-
1
1
-
2
-
-
1
-
-
4
3
-
2
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
1
1
-
-
-
1
-
4
3
-
2
1
-
-
-
-
1
1
-
-
-
-
-
-
-
-
439366
Foster
Synthesis of quinolinic acid b ...
Rattus norvegicus
J. Neurochem.
47
23-30
1986
-
-
-
-
-
-
1
1
1
1
-
1
-
2
-
-
-
-
-
2
-
1
2
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
1
1
-
1
-
-
-
-
-
2
-
1
2
-
1
-
-
-
1
1
-
-
-
-
-
-
-
-
439365
Koontz
Beef kidney 3-hydroxyanthranil ...
Bos taurus
J. Biol. Chem.
251
368-377
1976
-
-
-
-
-
-
-
-
-
2
1
-
-
2
-
-
1
-
-
2
1
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
1
-
-
-
-
1
-
2
1
2
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439370
Savage
-
The purification and some prop ...
Papio ursinus
Int. J. Biochem.
6
459-466
1975
-
-
-
-
-
-
1
5
-
1
1
1
1
1
-
-
1
-
-
1
1
1
5
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
5
-
1
1
1
1
-
-
1
-
1
1
1
5
1
1
-
-
-
1
-
-
-
-
-
-
-
-
-
439364
Decker
Purification and properties of ...
Bos taurus
J. Biol. Chem.
236
3076-3082
1961
1
-
-
-
-
-
1
1
-
5
-
-
1
1
-
-
1
-
-
1
1
2
1
-
-
1
-
-
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
1
-
1
-
5
-
-
1
-
-
1
-
1
1
2
1
-
-
1
-
-
-
1
1
-
-
-
-
-
-
-