Activating Compound | Comment | Organism | Structure |
---|---|---|---|
glycerol | 10% (v/v) glycerol slightly (1.2fold) stimulates vanillin production | Caulobacter segnis |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli T7 Express cells | Caulobacter segnis |
Protein Variants | Comment | Organism |
---|---|---|
A49P | the catalytic efficiency of the mutant is 1.6fold that of the wild type enzyme. The mutant exhibits better thermostability than that of wild type. Under temperatures lower than 30°C, mutant A49P retains almost all the activities within the shorter incubation time (less than 5 h). The mutant also shows better performance even at 35°C | Caulobacter segnis |
I234A | the mutant shows strongly decreased catalytic efficiency compared to the wild type enzyme | Caulobacter segnis |
Q390A | the catalytic efficiency of the mutant is 1.64fold that of the wild type enzyme. The mutant exhibits better thermostability than that of wild type | Caulobacter segnis |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Triton X-100 | 0.1% (v/v) Triton X-100 significantly inhibits vanillin production (to less than 40%) | Caulobacter segnis |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | 0.6 mM used in assay conditions | Caulobacter segnis |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-vinylguaiacol + O2 | Caulobacter segnis | - |
vanillin | - |
? | |
4-vinylguaiacol + O2 | Caulobacter segnis ATCC 21756 | - |
vanillin | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Caulobacter segnis | D5VGA0 | - |
- |
Caulobacter segnis ATCC 21756 | D5VGA0 | - |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA column chromatography and Superdex 200 gel filtration | Caulobacter segnis |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-vinylguaiacol + O2 | - |
Caulobacter segnis | vanillin | - |
? | |
4-vinylguaiacol + O2 | - |
Caulobacter segnis ATCC 21756 | vanillin | - |
? |
Subunits | Comment | Organism |
---|---|---|
? | x * 55000, SDS-PAGE | Caulobacter segnis |
Synonyms | Comment | Organism |
---|---|---|
carotenoid cleavage oxygenase | - |
Caulobacter segnis |
CSO2 | - |
Caulobacter segnis |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
10 | 15 | - |
Caulobacter segnis |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
5 | 30 | more than 80% activity between 5 and 30°C. Less than 10% of the maximum activity is retained when the temperature is higher than 30°C | Caulobacter segnis |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | 35 | the enzyme retains about 70-90% activity after approximately 3 h at 15-23°C. At 30°C, the enzyme maintains about 50% activity after 3 h incubation. The enzyme is inactivated after about 3 h at 35°C | Caulobacter segnis |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7.5 | - |
- |
Caulobacter segnis |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
6 | 10 | more than 80% activity between pH 6.0 and 10.0. Less than 10% of the maximum activity is retained when the pH is lower than 6.0 | Caulobacter segnis |