Any feedback?
Please rate this page
(literature.php)
(0/150)

BRENDA support

Literature summary for 1.13.11.78 extracted from

  • Gama, S.R.; Lo, B.S.Y.; Seguin, J.; Pallitsch, K.; Hammerschmidt, F.; Zechel, D.L.
    C-H bond cleavage is rate-limiting for oxidative C-P bond cleavage by the mixed valence diiron-dependent oxygenase PhnZ (2019), Biochemistry, 58, 5271-5280 .
    View publication on PubMed
Search for more literature for 1.13.11.78

Protein Variants

Protein Variants Comment Organism
E27A active site variant with severely reduced catalytic efficiency compared to the wild type enzyme uncultured bacterium
H62A active site variant with strongly reduced catalytic efficiency compared to the wild type enzyme uncultured bacterium
Y24F active site variant with strongly reduced catalytic efficiency compared to the wild type enzyme uncultured bacterium

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.063
-
 (R)-(2-amino-1-hydroxyethyl)phosphonate wild type enzyme, at pH 7.0 and 25°C uncultured bacterium
0.235
-
 (R)-(2-amino-1-hydroxyethyl)phosphonate mutant enzyme E27A, at pH 7.0 and 25°C uncultured bacterium
0.31
-
 (R)-(2-amino-1-hydroxyethyl)phosphonate mutant enzyme H62A, at pH 7.0 and 25°C uncultured bacterium
0.566
-
 (R)-(2-amino-1-hydroxyethyl)phosphonate mutant enzyme Y24F, at pH 7.0 and 25°C uncultured bacterium

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ dependent on uncultured bacterium

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
(R)-(2-amino-1-hydroxyethyl)phosphonate + O2 uncultured bacterium
-
 glycine + phosphate
-
 ?

Organism

Organism UniProt Comment Textmining
uncultured bacterium
-
-
-

Purification (Commentary)

Purification (Comment) Organism
-
 uncultured bacterium

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
(R)-(2-amino-1-hydroxyethyl)phosphonate + O2
-
 uncultured bacterium glycine + phosphate
-
 ?

Synonyms

Synonyms Comment Organism
phnZ
-
 uncultured bacterium

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.25
-
 (R)-(2-amino-1-hydroxyethyl)phosphonate mutant enzyme E27A, at pH 7.0 and 25°C uncultured bacterium
0.34
-
 (R)-(2-amino-1-hydroxyethyl)phosphonate mutant enzyme H62A, at pH 7.0 and 25°C uncultured bacterium
4.5
-
 (R)-(2-amino-1-hydroxyethyl)phosphonate mutant enzyme Y24F, at pH 7.0 and 25°C uncultured bacterium
6.8
-
 (R)-(2-amino-1-hydroxyethyl)phosphonate wild type enzyme, at pH 7.0 and 25°C uncultured bacterium

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 uncultured bacterium

kcat/KM [mM/s]

kcat/KM Value [1/mMs-1] kcat/KM Value Maximum [1/mMs-1] Substrate Comment Organism Structure
0.000106
-
 (R)-(2-amino-1-hydroxyethyl)phosphonate mutant enzyme E27A, at pH 7.0 and 25°C uncultured bacterium
1.1
-
 (R)-(2-amino-1-hydroxyethyl)phosphonate mutant enzyme H62A, at pH 7.0 and 25°C uncultured bacterium
7.9
-
 (R)-(2-amino-1-hydroxyethyl)phosphonate mutant enzyme Y24F, at pH 7.0 and 25°C uncultured bacterium
110
-
 (R)-(2-amino-1-hydroxyethyl)phosphonate wild type enzyme, at pH 7.0 and 25°C uncultured bacterium