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Literature summary for 1.13.11.85 extracted from

  • Schmitt, G.; Birke, J.; Jendrossek, D.
    Towards the understanding of the enzymatic cleavage of polyisoprene by the dihaem-dioxygenase RoxA (2019), AMB Express, 9, 166 .
    View publication on PubMedView publication on EuropePMC
Search for more literature for 1.13.11.85

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli S17-1 cells Steroidobacter cummioxidans

Protein Variants

Protein Variants Comment Organism
F301L the mutant shows 14% of wild type activity Steroidobacter cummioxidans
F301Y the mutant shows 21% of wild type activity Steroidobacter cummioxidans
F317A polyisoprene-cleaving activity of the variant is drastically reduced compared to the wild type enzyme Steroidobacter cummioxidans
F317L polyisoprene-cleaving activity of the variant is drastically reduced compared to the wild type enzyme Steroidobacter cummioxidans
F317Y polyisoprene-cleaving activity of the variant is drastically reduced compared to the wild type enzyme Steroidobacter cummioxidans

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
cis-1,4-polyisoprene + n O2 Steroidobacter cummioxidans
-
 n (4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal
-
 ?
cis-1,4-polyisoprene + n O2 Steroidobacter cummioxidans 35Y
-
 n (4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal
-
 ?

Organism

Organism UniProt Comment Textmining
Steroidobacter cummioxidans
-
 previously Xanthomonas sp. strain 35Y
-
Steroidobacter cummioxidans 35Y
-
 previously Xanthomonas sp. strain 35Y
-

Oxidation Stability

Oxidation Stability Organism
reoxygenation of the enzyme, that is deoxygenated by the addition of stoichiometric amounts of the reductant dithionite, can be achieved simply by incubation under an oxygen-containing gas atmosphere. Oxidized (deoxygenated) enzyme can also be reoxygenated by the presence of a slight molar excess of H2O2 within minutes. The enzyme retains its activity in the fully oxidized low-spin state Steroidobacter cummioxidans

Purification (Commentary)

Purification (Comment) Organism
Q-Sepharose column chromatography and hydroxyapatite column chromatography Steroidobacter cummioxidans

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cis-1,4-polyisoprene + n O2
-
 Steroidobacter cummioxidans n (4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal
-
 ?
cis-1,4-polyisoprene + n O2
-
 Steroidobacter cummioxidans 35Y n (4Z,8Z)-4,8-dimethyl-12-oxotrideca-4,8-dienal
-
 ?
additional information the enzyme does not feature a peroxidase activity Steroidobacter cummioxidans ?
-
-
additional information the enzyme does not feature a peroxidase activity Steroidobacter cummioxidans 35Y ?
-
-

Synonyms

Synonyms Comment Organism
RubA
-
 Steroidobacter cummioxidans
rubber oxygenase A
-
 Steroidobacter cummioxidans

Cofactor

Cofactor Comment Organism Structure
heme the enzyme harbors two heme centers Steroidobacter cummioxidans