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Literature summary for 1.13.11.87 extracted from

  • Vivod, R.; Andler, R.; Oetermann, S.; Altenhoff, A.L.; Seipel, N.; Holtkamp, M.; Hogeback, J.; Karst, U.; Steinbuechel, A.
    Characterization of the latex clearing protein of the poly(cis-1,4-isoprene) and poly(trans-1,4-isoprene) degrading bacterium Nocardia nova SH22a (2020), J. Gen. Appl. Microbiol., 65, 293-300 .
    View publication on PubMed
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Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli C41(DE3) cells Nocardia nova

Inhibitors

Inhibitors Comment Organism Structure
2,2'-bipyridyl 15% residual activity at 10 mM Nocardia nova
disodium 4,5-dihydroxy-1,3-benzenedisulfonate 45% residual activity at 10 mM Nocardia nova
EDTA 25% residual activity at 10 mM Nocardia nova
ethylxanthate 46% residual activity at 10 mM Nocardia nova
Phenanthroline 19% residual activity at 10 mM Nocardia nova

Metals/Ions

Metals/Ions Comment Organism Structure
Fe2+ metal cofactor Nocardia nova

Organism

Organism UniProt Comment Textmining
Nocardia nova
-
-
-
Nocardia nova SH22a
-
-
-

Purification (Commentary)

Purification (Comment) Organism
HisTrap column chromatography Nocardia nova

Source Tissue

Source Tissue Comment Organism Textmining

Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
0.03
-
 at pH 7.0 and 30°C Nocardia nova

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
cis-1,4-polyisoprene + n O2 the enzyme produces cis-oligo-isoprenoid molecules with a range of n = 4 (299 g/mol) to n = 37 (1423 g/mol) where n is equal to the number of isoprene units of the oligo-isoprenoid molecules Nocardia nova ?
-
 ?
cis-1,4-polyisoprene + n O2 the enzyme produces cis-oligo-isoprenoid molecules with a range of n = 4 (299 g/mol) to n = 37 (1423 g/mol) where n is equal to the number of isoprene units of the oligo-isoprenoid molecules Nocardia nova SH22a ?
-
 ?
trans-1,4-polyisoprene + n O2 the enzyme produces cis-oligo-isoprenoid molecules of n = 2 (231 g/mol) and n = 4 (299 g/mol) where n is equal to the number of isoprene units of the oligo-isoprenoid molecules Nocardia nova ?
-
 ?
trans-1,4-polyisoprene + n O2 the enzyme produces cis-oligo-isoprenoid molecules of n = 2 (231 g/mol) and n = 4 (299 g/mol) where n is equal to the number of isoprene units of the oligo-isoprenoid molecules Nocardia nova SH22a ?
-
 ?

Subunits

Subunits Comment Organism
? x * 40000, His6-tagged enzyme, SDS-PAGE Nocardia nova

Synonyms

Synonyms Comment Organism
latex-clearing protein
-
 Nocardia nova
Lcp
-
 Nocardia nova

Temperature Optimum [°C]

Temperature Optimum [°C] Temperature Optimum Maximum [°C] Comment Organism
30
-
-
 Nocardia nova

Temperature Range [°C]

Temperature Minimum [°C] Temperature Maximum [°C] Comment Organism
20 35 about 65% activity at 20°C, about 68% activity at 25°C, 100% activity at 30°C, about 85% activity at 35°C, about 45% activity at 40°C Nocardia nova

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
7
-
-
 Nocardia nova

pH Range

pH Minimum pH Maximum Comment Organism
6 7.5 about 40% activity at pH 5.0, about 80% activity at pH 6.0-6.5, 100% activity at pH 7.0, about 70% activity at pH 7.5, about 30% activity at pH 8.0, no activity at pH 9.0 and 10.0 Nocardia nova

Cofactor

Cofactor Comment Organism Structure
heme b
-
 Nocardia nova