BRENDA - Reference to 1.13.12.16; Id = 639224

3-(3,4-dihydroxyphenyl)-L-Ala

-

Cyberlindnera mrakii

5-hydroxytryptophan

-

Cyberlindnera mrakii

acetone

inhibits competitively against 2-nitropropane, no inhibitory effect against O2

Cyberlindnera mrakii

alpha-Naphthol

-

Cyberlindnera mrakii

catechol

-

Cyberlindnera mrakii

cytochrome c

competitive inhibitor

Cyberlindnera mrakii

epinephrine

competitive inhibitor

Cyberlindnera mrakii

hydroquinone

-

Cyberlindnera mrakii

additional information

inhibition by superoxide dismutase and various scavengers for superoxide; phenol, tryptophan, and tryptamine show no effect on the reaction

Cyberlindnera mrakii

NADH

competitive inhibitor. Oxygenation is inhibited approximately 86% by 0.1 mM NADH, although NAD+ and NADP+ are ineffective even at 1 mM. During the inhibition, NADH and NADPH are oxidized

Cyberlindnera mrakii

NADPH

competitive inhibitor. oxygenation is inhibited approximately 100% by 0.1 mM NADPH, respectively, although NAD+ and NADP+ are ineffective even at 1 mM. During the inhibition, NADH and NADPH are oxidized

Cyberlindnera mrakii

nitrite

inhibits noncompetitively against 2-nitropropane, functioned as a noncompetitive inhibitor against oxygen in the presence of excess 2-nitropropane (50 mM)

Cyberlindnera mrakii

phloroglucinol

-

Cyberlindnera mrakii

pyrogallol

-

Cyberlindnera mrakii

resorcinol

-

Cyberlindnera mrakii

serotonin

-

Cyberlindnera mrakii

Superoxide dismutase

-

Cyberlindnera mrakii

Thiophenol

-

Cyberlindnera mrakii

Tiron

i.e. pyrocatechol-3,5-disulfonate disodium salt

Cyberlindnera mrakii

0.277

-

O2

-

Cyberlindnera mrakii

19.2

-

2-Nitropropane

-

Cyberlindnera mrakii

-

Cyberlindnera mrakii

-

IF0 0895

-

Cyberlindnera mrakii IF0 0895

-

IF0 0895

-

purified to homogeneity

Cyberlindnera mrakii

ethylnitronate + O2 = acetaldehyde + nitrite + other products

superoxide as reactive intermediate

Cyberlindnera mrakii

ethylnitronate + O2 = acetaldehyde + nitrite + other products

ordered bi bi mechanism in which 2-nitropropane first combines with the enzyme and the enzyme 2-nitropropane complex reacts with oxygen to form a ternary complex

Cyberlindnera mrakii

2-nitropropane + O2

superoxide as reactive intermediate

639224

Cyberlindnera mrakii

acetone + HNO2

-

?

2-nitropropane + O2

superoxide as reactive intermediate

639224

Cyberlindnera mrakii IF0 0895

acetone + HNO2

-

?

2-nitropropane dioxygenase

-

Cyberlindnera mrakii

cytochrome c

cytochrome c also inhibits the reaction and half-inhibition is found in the presence of 17 microM cytochrome c. The addition of cytochrome c to the reaction mixture containing 2-nitropropane and 2-nitropropane dioxygenase causes an increase in absorbance at 550 nm indicating the reduction of cytochrome c

Cyberlindnera mrakii

cytochrome c

cytochrome c also inhibits the reaction and half-inhibition is found in the presence of 17 microM cytochrome c. The addition of cytochrome c to the reaction mixture containing 2-nitropropane and 2-nitropropane dioxygenase causes an increase in absorbance at 550 nm indicating the reduction of cytochrome c

Cyberlindnera mrakii

3-(3,4-dihydroxyphenyl)-L-Ala

-

Cyberlindnera mrakii

5-hydroxytryptophan

-

Cyberlindnera mrakii

acetone

inhibits competitively against 2-nitropropane, no inhibitory effect against O2

Cyberlindnera mrakii

alpha-Naphthol

-

Cyberlindnera mrakii

catechol

-

Cyberlindnera mrakii

cytochrome c

competitive inhibitor

Cyberlindnera mrakii

epinephrine

competitive inhibitor

Cyberlindnera mrakii

hydroquinone

-

Cyberlindnera mrakii

additional information

inhibition by superoxide dismutase and various scavengers for superoxide

Cyberlindnera mrakii

additional information

phenol, tryptophan, and tryptamine show no effect on the reaction

Cyberlindnera mrakii

NADH

competitive inhibitor. Oxygenation is inhibited approximately 86% by 0.1 mM NADH, although NAD+ and NADP+ are ineffective even at 1 mM. During the inhibition, NADH and NADPH are oxidized

Cyberlindnera mrakii

NADPH

competitive inhibitor. oxygenation is inhibited approximately 100% by 0.1 mM NADPH, respectively, although NAD+ and NADP+ are ineffective even at 1 mM. During the inhibition, NADH and NADPH are oxidized

Cyberlindnera mrakii

nitrite

inhibits noncompetitively against 2-nitropropane, functioned as a noncompetitive inhibitor against oxygen in the presence of excess 2-nitropropane (50 mM)

Cyberlindnera mrakii

phloroglucinol

-

Cyberlindnera mrakii

pyrogallol

-

Cyberlindnera mrakii

resorcinol

-

Cyberlindnera mrakii

serotonin

-

Cyberlindnera mrakii

Superoxide dismutase

-

Cyberlindnera mrakii

Thiophenol

-

Cyberlindnera mrakii

Tiron

i.e. pyrocatechol-3,5-disulfonate disodium salt

Cyberlindnera mrakii

0.277

-

O2

-

Cyberlindnera mrakii

19.2

-

2-Nitropropane

-

Cyberlindnera mrakii

purified to homogeneity

Cyberlindnera mrakii

2-nitropropane + O2

superoxide as reactive intermediate

639224

Cyberlindnera mrakii

acetone + HNO2

-

?

2-nitropropane + O2

superoxide as reactive intermediate

639224

Cyberlindnera mrakii IF0 0895

acetone + HNO2

-

?

745219

Vercammen

Pseudomonas aeruginosa LysR P ...

Pseudomonas aeruginosa

J. Bacteriol.

197

1026-1039

2015

-

1

-

1

-

1

-

3

-

1

-

5

-

1

-

1

-

1

-

1

-

1

-

2

-

745306

Salvi

The combined structural and k ...

Pseudomonas aeruginosa

J. Biol. Chem.

289

23764-23775

2014

-

1

-

4

-

1

-

10

-

1

-

8

1

5

1

-

3

1

-

1

-

1

-

4

-

1

-

1

-

8

1

-

3

1

-

3

-

6

727018

Smitherman

Evidence for a transient perox ...

Cyberlindnera saturnus

Biochemistry

52

2694-2704

2013

-

6

-

3

-

2

-

1

2

-

5

1

-

6

-

2

-

2

-

5

1

-

6

728077

Klinkenberg

Rv1894c is a novel hypoxia-ind ...

Mycobacterium tuberculosis

J. Infect. Dis.

207

1525-1534

2013

-

1

-

1

-

1

-

10

-

1

-

1

-

2

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

1

-

726808

Francis

A novel activity for fungal ni ...

Cyberlindnera saturnus, Neurospora crassa

Arch. Biochem. Biophys.

521

84-89

2012

-

3

-

10

-

3

-

4

2

-

3

2

-

3

-

3

-

2

-

3

2

-

3

-

3

724175

Li

Crystal structure and site-dir ...

Streptomyces ansochromogenes

Biochem. Biophys. Res. Commun.

405

344-348

2011

-

1

3

-

2

-

3

-

2

-

1

-

1

-

1

3

-

3

-

1

-

695891

Gadda

Nitronate monooxygenase, a mod ...

Cyberlindnera saturnus, Neurospora crassa, Pseudomonas aeruginosa, Cyberlindnera saturnus mrakii

Arch. Biochem. Biophys.

493

53-61

2009

-

1

-

3

-

13

-

6

-

3

-

16

1

3

-

3

-

3

-

3

-

1

-

3

-

13

-

16

1

3

-

3

-

696315

Francis

Inflated kinetic isotope effec ...

Neurospora crassa

Biochemistry

48

2403-2410

2009

-

6

-

1

-

1

-

684712

Mijatovic

Oxidation of alkyl nitronates ...

Cyberlindnera mrakii

Arch. Biochem. Biophys.

473

61-68

2008

-

1

-

1

-

1

-

6

-

1

-

2

-

8

-

1

-

2

-

1

-

1

-

1

-

1

-

1

-

2

-

8

-

1

-

2

-

6

685283

Francis

The nonoxidative conversion of ...

Neurospora crassa

Biochemistry

47

9136-9144

2008

-

1

-

2

-

1

6

-

4

-

1

-

5

-

1

-

2

1

-

2

-

1

-

2

-

1

2

6

-

1

-

5

-

1

-

2

1

-

4

672094

Francis

Probing the chemical steps of ...

Neurospora crassa

Biochemistry

45

13889-13898

2006

-

1

11

-

1

-

1

-

4

-

2

1

-

5

1

-

1

-

1

-

1

-

11

-

1

-

4

-

1

-

5

1

-

7

674590

Ha

Crystal structure of 2-nitropr ...

Pseudomonas aeruginosa

J. Biol. Chem.

281

18660-18667

2006

-

1

4

-

1

-

1

-

5

-

1

-

2

1

-

1

-

1

-

1

4

-

1

-

1

-

1

-

2

1

-

1

-

656289

Francis

Involvement of a flavosemiquin ...

Neurospora crassa

J. Biol. Chem.

280

5195-5204

2005

-

3

-

11

-

2

4

1

-

7

-

3

-

5

1

33

2

1

2

-

28

2

1

-

3

-

3

-

11

-

2

4

1

-

3

-

5

1

33

2

-

28

2

1

-

10

639233

Gadda

Substrate specificity of a nit ...

Fusarium oxysporum

Arch. Biochem. Biophys.

363

309-313

1999

-

1

-

1

-

3

-

14

-

1

-

2

-

1

2

-

1

-

2

-

1

-

14

-

1

-

2

-

639232

Gorlatova

Purification, characterization ...

Neurospora crassa

Appl. Environ. Microbiol.

64

1029-1033

1998

-

8

10

-

2

-

10

-

2

-

1

-

20

1

-

9

1

-

2

-

2

-

15

-

10

-

2

-

2

-

1

-

20

1

-

9

1

-

698266

Balogh-Hergovich

Copper-mediated oxygenation of ...

Cyberlindnera mrakii

Inorg. Chem.

37

6535-6537

1998

-

1

-

1

-

1

-

1

-

1

-

1

-

697294

Balogh-Hergovich

-

Copper mediated conversion of ...

Cyberlindnera mrakii

Chem. Lett.

25

573-574

1996

-

1

-

8

-

8

-

639222

Kido

-

2-Nitropropane dioxygenase fro ...

Cyberlindnera mrakii

Agric. Biol. Chem.

48

2549-2554

1984

-

21

-

2

1

-

2

-

2

-

2

-

23

2

1

-

4

-

2

-

2

-

21

-

2

1

-

2

-

2

-

23

2

-

4

-

639226

Kido

-

Characterization of primary ni ...

Cyberlindnera mrakii

Agric. Biol. Chem.

48

1361-1362

1984

-

1

-

1

-

1

-

4

-

1

-

1

-

1

-

1

-

1

-

4

-

639227

Kido

Oxidation of anionic nitroalka ...

Cyberlindnera mrakii

Arch. Biochem. Biophys.

234

468-475

1984

-

11

2

-

1

-

9

-

1

-

8

-

1

-

1

-

1

-

1

-

14

-

2

-

1

-

1

-

8

-

1

-

639224

Kido

Properties of 2-nitropropane d ...

Cyberlindnera mrakii, Cyberlindnera mrakii IF0 0895

J. Biol. Chem.

253

226-232

1978

-

19

2

-

11

-

1

2

-

2

-

1

-

1

-

1

-

20

-

2

-

1

-

2

-

639225

Kido

Purification and properties of ...

Fusarium oxysporum

J. Bacteriol.

133

53-58

1978

-

10

6

-

2

-

4

-

2

-

1

14

1

2

6

-

1

-

1

3

-

3

-

11

-

6

-

2

-

2

-

1

14

1

2

6

-

1

-

1

-

639223

Kido

A new oxygenase, 2-nitropropan ...

Cyberlindnera mrakii, Cyberlindnera mrakii IF0 0895

J. Biol. Chem.

251

6994-7000

1976

3

-

3

13

7

-

1

4

-

8

-

1

-

17

1

-

2

-

2

-

3

-

2

-

3

-

18

-

7

-

1

4

-

1

-

17

1

-

2

-

639235

Kido

Purification and properties of ...

Cyberlindnera mrakii

J. Bacteriol.

126

1261-1265

1976

-

5

-

1

-

1

-

5

1

-

2

-

1

-

1

-

5

1

-

2

-

Properties of 2-nitropropane dioxygenase of Hansenula mrakii. Formation and participation of superoxide

Data extracted from this reference: