BRENDA - Enzyme Database
show all sequences of 1.13.12.16

Properties of 2-nitropropane dioxygenase of Hansenula mrakii. Formation and participation of superoxide

Kido, T.; Soda, K.; Asada, K.; J. Biol. Chem. 253, 226-232 (1978)

Data extracted from this reference:

Inhibitors
Inhibitors
Commentary
Organism
Structure
3-(3,4-dihydroxyphenyl)-L-Ala
-
Cyberlindnera mrakii
5-hydroxytryptophan
-
Cyberlindnera mrakii
acetone
inhibits competitively against 2-nitropropane, no inhibitory effect against O2
Cyberlindnera mrakii
alpha-Naphthol
-
Cyberlindnera mrakii
catechol
-
Cyberlindnera mrakii
cytochrome c
competitive inhibitor
Cyberlindnera mrakii
epinephrine
competitive inhibitor
Cyberlindnera mrakii
hydroquinone
-
Cyberlindnera mrakii
additional information
inhibition by superoxide dismutase and various scavengers for superoxide; phenol, tryptophan, and tryptamine show no effect on the reaction
Cyberlindnera mrakii
NADH
competitive inhibitor. Oxygenation is inhibited approximately 86% by 0.1 mM NADH, although NAD+ and NADP+ are ineffective even at 1 mM. During the inhibition, NADH and NADPH are oxidized
Cyberlindnera mrakii
NADPH
competitive inhibitor. oxygenation is inhibited approximately 100% by 0.1 mM NADPH, respectively, although NAD+ and NADP+ are ineffective even at 1 mM. During the inhibition, NADH and NADPH are oxidized
Cyberlindnera mrakii
nitrite
inhibits noncompetitively against 2-nitropropane, functioned as a noncompetitive inhibitor against oxygen in the presence of excess 2-nitropropane (50 mM)
Cyberlindnera mrakii
phloroglucinol
-
Cyberlindnera mrakii
pyrogallol
-
Cyberlindnera mrakii
resorcinol
-
Cyberlindnera mrakii
serotonin
-
Cyberlindnera mrakii
Superoxide dismutase
-
Cyberlindnera mrakii
Thiophenol
-
Cyberlindnera mrakii
Tiron
i.e. pyrocatechol-3,5-disulfonate disodium salt
Cyberlindnera mrakii
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.277
-
O2
-
Cyberlindnera mrakii
19.2
-
2-Nitropropane
-
Cyberlindnera mrakii
Organism
Organism
UniProt
Commentary
Textmining
Cyberlindnera mrakii
-
-
-
Cyberlindnera mrakii
-
IF0 0895
-
Cyberlindnera mrakii IF0 0895
-
IF0 0895
-
Purification (Commentary)
Purification (Commentary)
Organism
purified to homogeneity
Cyberlindnera mrakii
Reaction
Reaction
Commentary
Organism
Reaction ID
ethylnitronate + O2 = acetaldehyde + nitrite + other products
superoxide as reactive intermediate
Cyberlindnera mrakii
ethylnitronate + O2 = acetaldehyde + nitrite + other products
ordered bi bi mechanism in which 2-nitropropane first combines with the enzyme and the enzyme 2-nitropropane complex reacts with oxygen to form a ternary complex
Cyberlindnera mrakii
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
2-nitropropane + O2
superoxide as reactive intermediate
639224
Cyberlindnera mrakii
acetone + HNO2
-
-
-
?
2-nitropropane + O2
superoxide as reactive intermediate
639224
Cyberlindnera mrakii IF0 0895
acetone + HNO2
-
-
-
?
Synonyms
Synonyms
Commentary
Organism
2-nitropropane dioxygenase
-
Cyberlindnera mrakii
Cofactor
Cofactor
Commentary
Organism
Structure
cytochrome c
cytochrome c also inhibits the reaction and half-inhibition is found in the presence of 17 microM cytochrome c. The addition of cytochrome c to the reaction mixture containing 2-nitropropane and 2-nitropropane dioxygenase causes an increase in absorbance at 550 nm indicating the reduction of cytochrome c
Cyberlindnera mrakii
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
cytochrome c
cytochrome c also inhibits the reaction and half-inhibition is found in the presence of 17 microM cytochrome c. The addition of cytochrome c to the reaction mixture containing 2-nitropropane and 2-nitropropane dioxygenase causes an increase in absorbance at 550 nm indicating the reduction of cytochrome c
Cyberlindnera mrakii
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
3-(3,4-dihydroxyphenyl)-L-Ala
-
Cyberlindnera mrakii
5-hydroxytryptophan
-
Cyberlindnera mrakii
acetone
inhibits competitively against 2-nitropropane, no inhibitory effect against O2
Cyberlindnera mrakii
alpha-Naphthol
-
Cyberlindnera mrakii
catechol
-
Cyberlindnera mrakii
cytochrome c
competitive inhibitor
Cyberlindnera mrakii
epinephrine
competitive inhibitor
Cyberlindnera mrakii
hydroquinone
-
Cyberlindnera mrakii
additional information
inhibition by superoxide dismutase and various scavengers for superoxide
Cyberlindnera mrakii
additional information
phenol, tryptophan, and tryptamine show no effect on the reaction
Cyberlindnera mrakii
NADH
competitive inhibitor. Oxygenation is inhibited approximately 86% by 0.1 mM NADH, although NAD+ and NADP+ are ineffective even at 1 mM. During the inhibition, NADH and NADPH are oxidized
Cyberlindnera mrakii
NADPH
competitive inhibitor. oxygenation is inhibited approximately 100% by 0.1 mM NADPH, respectively, although NAD+ and NADP+ are ineffective even at 1 mM. During the inhibition, NADH and NADPH are oxidized
Cyberlindnera mrakii
nitrite
inhibits noncompetitively against 2-nitropropane, functioned as a noncompetitive inhibitor against oxygen in the presence of excess 2-nitropropane (50 mM)
Cyberlindnera mrakii
phloroglucinol
-
Cyberlindnera mrakii
pyrogallol
-
Cyberlindnera mrakii
resorcinol
-
Cyberlindnera mrakii
serotonin
-
Cyberlindnera mrakii
Superoxide dismutase
-
Cyberlindnera mrakii
Thiophenol
-
Cyberlindnera mrakii
Tiron
i.e. pyrocatechol-3,5-disulfonate disodium salt
Cyberlindnera mrakii
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.277
-
O2
-
Cyberlindnera mrakii
19.2
-
2-Nitropropane
-
Cyberlindnera mrakii
Purification (Commentary) (protein specific)
Commentary
Organism
purified to homogeneity
Cyberlindnera mrakii
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
2-nitropropane + O2
superoxide as reactive intermediate
639224
Cyberlindnera mrakii
acetone + HNO2
-
-
-
?
2-nitropropane + O2
superoxide as reactive intermediate
639224
Cyberlindnera mrakii IF0 0895
acetone + HNO2
-
-
-
?
Other publictions for EC 1.13.12.16
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
745219
Vercammen
Pseudomonas aeruginosa LysR P ...
Pseudomonas aeruginosa
J. Bacteriol.
197
1026-1039
2015
-
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1
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1
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1
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2
2
-
-
-
745306
Salvi
The combined structural and k ...
Pseudomonas aeruginosa
J. Biol. Chem.
289
23764-23775
2014
-
-
1
1
-
-
-
4
-
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1
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10
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1
1
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8
1
5
1
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3
1
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1
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1
1
1
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4
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1
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1
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8
1
1
-
-
3
1
-
-
-
-
3
3
-
6
6
727018
Smitherman
Evidence for a transient perox ...
Cyberlindnera saturnus
Biochemistry
52
2694-2704
2013
-
-
-
-
-
-
-
6
-
-
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-
3
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2
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1
2
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5
1
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6
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2
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2
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5
1
-
-
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-
-
-
-
6
6
728077
Klinkenberg
Rv1894c is a novel hypoxia-ind ...
Mycobacterium tuberculosis
J. Infect. Dis.
207
1525-1534
2013
-
-
1
-
1
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1
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10
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1
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1
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2
1
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1
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1
1
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1
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1
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1
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1
-
1
-
-
-
-
-
-
-
1
-
-
1
-
-
726808
Francis
A novel activity for fungal ni ...
Cyberlindnera saturnus, Neurospora crassa
Arch. Biochem. Biophys.
521
84-89
2012
-
-
-
-
-
-
-
3
-
-
-
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10
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2
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3
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2
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3
2
-
-
-
-
3
3
-
3
3
724175
Li
Crystal structure and site-dir ...
Streptomyces ansochromogenes
Biochem. Biophys. Res. Commun.
405
344-348
2011
-
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1
1
3
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2
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3
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1
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-
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-
-
-
-
-
695891
Gadda
Nitronate monooxygenase, a mod ...
Cyberlindnera saturnus, Neurospora crassa, Pseudomonas aeruginosa, Cyberlindnera saturnus mrakii
Arch. Biochem. Biophys.
493
53-61
2009
-
-
-
-
-
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-
1
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3
-
13
-
6
-
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3
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16
1
3
3
-
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3
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3
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3
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1
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3
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13
-
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16
1
3
-
-
-
3
-
-
-
-
-
-
-
-
-
696315
Francis
Inflated kinetic isotope effec ...
Neurospora crassa
Biochemistry
48
2403-2410
2009
-
-
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6
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1
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684712
Mijatovic
Oxidation of alkyl nitronates ...
Cyberlindnera mrakii
Arch. Biochem. Biophys.
473
61-68
2008
-
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1
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1
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1
1
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6
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1
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2
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8
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1
1
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1
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1
1
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1
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1
1
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1
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2
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8
-
1
-
-
-
2
-
-
-
-
-
-
-
6
6
685283
Francis
The nonoxidative conversion of ...
Neurospora crassa
Biochemistry
47
9136-9144
2008
-
-
1
-
2
-
1
6
-
-
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4
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1
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1
1
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1
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2
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1
2
6
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1
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5
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1
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2
1
1
-
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4
4
672094
Francis
Probing the chemical steps of ...
Neurospora crassa
Biochemistry
45
13889-13898
2006
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1
11
-
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1
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1
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11
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1
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4
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1
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5
1
1
-
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7
7
674590
Ha
Crystal structure of 2-nitropr ...
Pseudomonas aeruginosa
J. Biol. Chem.
281
18660-18667
2006
-
-
1
1
4
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1
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1
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5
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1
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1
1
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2
1
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1
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656289
Francis
Involvement of a flavosemiquin ...
Neurospora crassa
J. Biol. Chem.
280
5195-5204
2005
-
-
3
-
-
-
-
11
-
2
4
1
-
7
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3
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5
1
33
2
1
2
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28
2
1
-
3
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3
3
-
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11
-
2
4
1
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3
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5
1
33
2
2
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-
28
2
1
-
-
-
-
-
-
10
10
639233
Gadda
Substrate specificity of a nit ...
Fusarium oxysporum
Arch. Biochem. Biophys.
363
309-313
1999
-
-
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1
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3
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14
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1
1
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1
2
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1
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2
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14
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1
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2
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639232
Gorlatova
Purification, characterization ...
Neurospora crassa
Appl. Environ. Microbiol.
64
1029-1033
1998
-
-
-
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8
10
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2
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10
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2
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1
1
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20
1
1
1
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9
1
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2
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15
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10
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1
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20
1
1
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9
1
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698266
Balogh-Hergovich
Copper-mediated oxygenation of ...
Cyberlindnera mrakii
Inorg. Chem.
37
6535-6537
1998
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1
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1
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697294
Balogh-Hergovich
-
Copper mediated conversion of ...
Cyberlindnera mrakii
Chem. Lett.
25
573-574
1996
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1
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8
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639222
Kido
-
2-Nitropropane dioxygenase fro ...
Cyberlindnera mrakii
Agric. Biol. Chem.
48
2549-2554
1984
-
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21
-
2
2
1
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2
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2
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2
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23
2
1
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4
-
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2
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2
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21
-
2
2
1
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2
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2
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23
2
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4
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639226
Kido
-
Characterization of primary ni ...
Cyberlindnera mrakii
Agric. Biol. Chem.
48
1361-1362
1984
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1
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1
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1
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4
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1
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-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
639227
Kido
Oxidation of anionic nitroalka ...
Cyberlindnera mrakii
Arch. Biochem. Biophys.
234
468-475
1984
-
-
-
-
-
-
11
2
-
1
-
-
-
9
-
-
1
1
-
-
-
-
8
-
1
-
-
-
-
1
-
-
1
-
-
-
-
-
-
1
-
-
-
-
14
-
2
-
1
-
-
-
-
-
1
-
-
-
-
8
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
639224
Kido
Properties of 2-nitropropane d ...
Cyberlindnera mrakii, Cyberlindnera mrakii IF0 0895
J. Biol. Chem.
253
226-232
1978
-
-
-
-
-
-
19
2
-
-
-
-
-
11
-
-
1
2
-
-
-
-
2
-
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
20
-
2
-
-
-
-
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
639225
Kido
Purification and properties of ...
Fusarium oxysporum
J. Bacteriol.
133
53-58
1978
-
-
-
-
-
-
10
6
-
-
2
-
-
4
-
-
2
-
-
1
1
1
14
1
1
2
2
6
-
1
-
1
3
-
-
-
-
-
-
3
-
-
-
-
11
-
6
-
-
2
-
-
-
-
2
-
1
1
1
14
1
2
2
6
-
1
-
1
-
-
-
-
-
-
-
639223
Kido
A new oxygenase, 2-nitropropan ...
Cyberlindnera mrakii, Cyberlindnera mrakii IF0 0895
J. Biol. Chem.
251
6994-7000
1976
3
-
-
-
-
3
13
7
-
1
4
-
-
8
-
-
1
-
-
-
-
-
17
1
1
1
1
-
-
2
2
-
2
-
-
-
3
-
-
2
-
-
3
-
18
-
7
-
1
4
-
-
-
-
1
-
-
-
-
17
1
1
1
-
-
2
2
-
-
-
-
-
-
-
-
639235
Kido
Purification and properties of ...
Cyberlindnera mrakii
J. Bacteriol.
126
1261-1265
1976
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-
-
-
-
-
-
-
-
-
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-
-
5
-
-
1
-
-
1
1
-
5
1
1
1
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
1
-
5
1
1
-
-
-
2
-
-
-
-
-
-
-
-
-