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Literature summary for 1.13.12.24 extracted from

  • Inouye, S.; Sahara-Miura, Y.
    Expression and characterization of EF-hand I loop mutants of aequorin replaced with other loop sequences of Ca2+-binding proteins an approach to studying the EF-hand motif of proteins (2016), J. Biochem., 160, 59-68 .
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
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Aequorea victoria

Protein Variants

Protein Variants Comment Organism
additional information replacement of the loop sequence of EF-hand I with other known loop sequences of Ca2+-binding proteins. Mutants replaced with the EF-hand I and EF-hand III from photoproteins show sufficient luminescence activity, but mutants replaced by EF-hands from Renilla luciferin-binding protein and calmodulin do not. The Ca2+-binding affinity to aequorin is reduced by a positive charge at the second position of the canonical EF-hand motif sequence Aequorea victoria

Organism

Organism UniProt Comment Textmining
Aequorea victoria P02592
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