BRENDA - Enzyme Database show
show all sequences of 1.13.12.3

Purification and characterization of the flavoprotein tryptophan 2-monooxygenase expressed at high levels in Escherichia coli

Emanuele, J.J.; Heasley, C.J.; Fitzpatrick, P.F.; Arch. Biochem. Biophys. 316, 241-248 (1995)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
-
Pseudomonas savastanoi
Inhibitors
Inhibitors
Commentary
Organism
Structure
2-methyl methanethiolsulfonate
-
Pseudomonas savastanoi
D-methionine
competitive inhibition
Pseudomonas savastanoi
D-phenylalanine
competitive inhibition
Pseudomonas savastanoi
D-tryptophan
competitive inhibitor
Pseudomonas savastanoi
Diethylpyrocarbonate
-
Pseudomonas savastanoi
diethylthiocarbonate
-
Pseudomonas savastanoi
Hydrocinnamic acid
competitive inhibition
Pseudomonas savastanoi
Indole-3-acetamide
-
Pseudomonas savastanoi
indole-3-acetic acid
-
Pseudomonas savastanoi
N-ethylmaleimide
-
Pseudomonas savastanoi
phenylacetamide
competitive inhibition
Pseudomonas savastanoi
Phenylethylamine
competitive inhibition
Pseudomonas savastanoi
phenyllactic acid
competitive inhibition
Pseudomonas savastanoi
phenylpyruvic acid
competitive inhibition
Pseudomonas savastanoi
tryptamine
competitive inhibition
Pseudomonas savastanoi
Vinylglycine
-
Pseudomonas savastanoi
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.036
-
L-tryptophan
-
Pseudomonas savastanoi
1.3
-
D,L-3-phenyl-2-hydrazinopropanoic acid
-
Pseudomonas savastanoi
2.4
-
L-phenylalanine
-
Pseudomonas savastanoi
22.4
-
L-methionine
-
Pseudomonas savastanoi
22.6
-
D,L-N-methyl-phenylalanine
-
Pseudomonas savastanoi
22.6
-
L-alanine
-
Pseudomonas savastanoi
32
-
D,L-3-pyridylalanine
-
Pseudomonas savastanoi
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-tryptophan + O2
Pseudomonas savastanoi
-
indole-3-acetamide + CO2 + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Pseudomonas savastanoi
-
-
-
Purification (Commentary)
Commentary
Organism
-
Pseudomonas savastanoi
Specific Activity [micromol/min/mg]
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
12.7
-
-
Pseudomonas savastanoi
Storage Stability
Storage Stability
Organism
-20C, several weeks without significant loss in activity
Pseudomonas savastanoi
4C, several weeks without significant loss in activity
Pseudomonas savastanoi
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D,L-3-phenyl-2-hydrazinopropanoic acid + O2
-
439347
Pseudomonas savastanoi
2-phenylacetohydrazide + CO2 + H2O
-
-
-
?
D,L-3-pyridylalanine + O2
-
439347
Pseudomonas savastanoi
2-pyridylacetamide + CO2 + H2O
-
-
-
?
D,L-N-methyl-phenylalanine + O2
-
439347
Pseudomonas savastanoi
N-methyl-2-phenylacetamide + CO2 + H2O
-
-
-
?
L-alanine + O2
-
439347
Pseudomonas savastanoi
acetamide + CO2 + H2O
-
-
-
?
L-methionine + O2
-
439347
Pseudomonas savastanoi
3-(methylthio)propanamide + CO2 + H2O
-
-
-
?
L-phenylalanine + O2
-
439347
Pseudomonas savastanoi
phenylacetamide + CO2 + H2O
-
-
-
?
L-tryptophan + O2
-
439347
Pseudomonas savastanoi
indole-3-acetamide + CO2 + H2O
-
-
-
?
Temperature Range [C]
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
35
45
-
Pseudomonas savastanoi
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.0000167
-
D,L-3-phenyl-2-hydrazinopropanoic acid
-
Pseudomonas savastanoi
0.000317
-
D,L-N-methyl-phenylalanine
-
Pseudomonas savastanoi
0.000317
-
L-alanine
-
Pseudomonas savastanoi
0.00075
-
D,L-3-pyridylalanine
-
Pseudomonas savastanoi
0.00155
-
L-methionine
-
Pseudomonas savastanoi
0.00305
-
L-phenylalanine
-
Pseudomonas savastanoi
0.00367
-
L-tryptophan
-
Pseudomonas savastanoi
pH Range
pH Minimum
pH Maximum
Commentary
Organism
4.5
11
-
Pseudomonas savastanoi
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
-
Pseudomonas savastanoi
Cloned(Commentary) (protein specific)
Commentary
Organism
-
Pseudomonas savastanoi
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
-
Pseudomonas savastanoi
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
2-methyl methanethiolsulfonate
-
Pseudomonas savastanoi
D-methionine
competitive inhibition
Pseudomonas savastanoi
D-phenylalanine
competitive inhibition
Pseudomonas savastanoi
D-tryptophan
competitive inhibitor
Pseudomonas savastanoi
Diethylpyrocarbonate
-
Pseudomonas savastanoi
diethylthiocarbonate
-
Pseudomonas savastanoi
Hydrocinnamic acid
competitive inhibition
Pseudomonas savastanoi
Indole-3-acetamide
-
Pseudomonas savastanoi
indole-3-acetic acid
-
Pseudomonas savastanoi
N-ethylmaleimide
-
Pseudomonas savastanoi
phenylacetamide
competitive inhibition
Pseudomonas savastanoi
Phenylethylamine
competitive inhibition
Pseudomonas savastanoi
phenyllactic acid
competitive inhibition
Pseudomonas savastanoi
phenylpyruvic acid
competitive inhibition
Pseudomonas savastanoi
tryptamine
competitive inhibition
Pseudomonas savastanoi
Vinylglycine
-
Pseudomonas savastanoi
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.036
-
L-tryptophan
-
Pseudomonas savastanoi
1.3
-
D,L-3-phenyl-2-hydrazinopropanoic acid
-
Pseudomonas savastanoi
2.4
-
L-phenylalanine
-
Pseudomonas savastanoi
22.4
-
L-methionine
-
Pseudomonas savastanoi
22.6
-
D,L-N-methyl-phenylalanine
-
Pseudomonas savastanoi
22.6
-
L-alanine
-
Pseudomonas savastanoi
32
-
D,L-3-pyridylalanine
-
Pseudomonas savastanoi
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-tryptophan + O2
Pseudomonas savastanoi
-
indole-3-acetamide + CO2 + H2O
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Pseudomonas savastanoi
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [mol/min/mg]
Specific Activity Maximum [mol/min/mg]
Commentary
Organism
12.7
-
-
Pseudomonas savastanoi
Storage Stability (protein specific)
Storage Stability
Organism
-20C, several weeks without significant loss in activity
Pseudomonas savastanoi
4C, several weeks without significant loss in activity
Pseudomonas savastanoi
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
D,L-3-phenyl-2-hydrazinopropanoic acid + O2
-
439347
Pseudomonas savastanoi
2-phenylacetohydrazide + CO2 + H2O
-
-
-
?
D,L-3-pyridylalanine + O2
-
439347
Pseudomonas savastanoi
2-pyridylacetamide + CO2 + H2O
-
-
-
?
D,L-N-methyl-phenylalanine + O2
-
439347
Pseudomonas savastanoi
N-methyl-2-phenylacetamide + CO2 + H2O
-
-
-
?
L-alanine + O2
-
439347
Pseudomonas savastanoi
acetamide + CO2 + H2O
-
-
-
?
L-methionine + O2
-
439347
Pseudomonas savastanoi
3-(methylthio)propanamide + CO2 + H2O
-
-
-
?
L-phenylalanine + O2
-
439347
Pseudomonas savastanoi
phenylacetamide + CO2 + H2O
-
-
-
?
L-tryptophan + O2
-
439347
Pseudomonas savastanoi
indole-3-acetamide + CO2 + H2O
-
-
-
?
Temperature Range [C] (protein specific)
Temperature Minimum [C]
Temperature Maximum [C]
Commentary
Organism
35
45
-
Pseudomonas savastanoi
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.0000167
-
D,L-3-phenyl-2-hydrazinopropanoic acid
-
Pseudomonas savastanoi
0.000317
-
D,L-N-methyl-phenylalanine
-
Pseudomonas savastanoi
0.000317
-
L-alanine
-
Pseudomonas savastanoi
0.00075
-
D,L-3-pyridylalanine
-
Pseudomonas savastanoi
0.00155
-
L-methionine
-
Pseudomonas savastanoi
0.00305
-
L-phenylalanine
-
Pseudomonas savastanoi
0.00367
-
L-tryptophan
-
Pseudomonas savastanoi
pH Range (protein specific)
pH Minimum
pH Maximum
Commentary
Organism
4.5
11
-
Pseudomonas savastanoi
Other publictions for EC 1.13.12.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746273
Zhang
Regulation of oncogene expres ...
Agrobacterium tumefaciens, Agrobacterium tumefaciens C58
PLoS Pathog.
11
1-27
2015
-
-
1
-
-
-
-
-
-
-
-
2
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
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1
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2
-
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-
-
2
-
-
-
-
-
-
-
-
-
1
2
2
1
-
-
745813
Yin
Characterization of a tryptop ...
Puccinia graminis f. sp. tritici, Puccinia graminis f. sp. tritici CRL 75-36-700-3
Mol. Plant Microbe Interact.
27
227-235
2014
-
-
1
-
-
-
-
-
-
-
-
2
-
10
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
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1
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2
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-
-
2
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
746257
Mujahid
Aniline is an inducer, and no ...
Rubrivivax benzoatilyticus, Rubrivivax benzoatilyticus JA2
PLoS ONE
9
e87503
2014
-
-
1
-
-
-
-
-
-
-
-
2
-
7
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
1
-
-
-
1
-
-
-
1
2
2
1
-
-
727017
Gaweska
Structure of the flavoprotein ...
Pseudomonas savastanoi
Biochemistry
52
2620-2626
2013
-
-
1
1
3
-
-
5
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
1
-
-
3
1
-
-
1
-
-
-
-
-
1
1
1
3
-
-
-
-
5
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
3
1
-
-
-
-
-
-
-
5
5
726752
Mujahid
Production of indole-3-acetic ...
Rubrivivax benzoatilyticus
Appl. Microbiol. Biotechnol.
89
1001-1008
2011
-
-
-
-
-
-
-
-
-
-
-
-
-
3
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
1
1
-
-
-
672116
Ralph
Mechanistic studies of the fla ...
Pseudomonas savastanoi
Biochemistry
45
15844-15852
2006
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
1
-
-
-
-
-
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
654682
Sobrado
Analysis of the role of the ac ...
Pseudomonas savastanoi
Biochemistry
42
13826-13832
2003
-
-
-
-
2
-
2
6
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
3
-
-
-
-
6
-
-
-
-
-
-
-
2
-
-
2
6
6
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
654683
Sobrado
Identification of Tyr413 as an ...
Pseudomonas savastanoi
Biochemistry
42
13833-13838
2003
-
-
-
-
2
-
2
6
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
3
-
-
-
-
6
-
-
-
-
-
-
-
2
-
-
2
6
6
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
3
-
-
-
-
-
-
-
-
-
-
439346
Sobrado
Analysis of the roles of amino ...
Pseudomonas savastanoi
Arch. Biochem. Biophys.
402
24-30
2002
-
-
-
-
3
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439349
Lemcke
The ORF8 gene product of Agrob ...
Agrobacterium rhizogenes
Mol. Plant Microbe Interact.
13
787-790
2000
-
-
1
-
-
-
-
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439348
Gadda
Characterization of 2-oxo-3-pe ...
Pseudomonas savastanoi
Biochemistry
38
5822-5828
1999
-
-
1
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
1
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439347
Emanuele
Purification and characterizat ...
Pseudomonas savastanoi
Arch. Biochem. Biophys.
316
241-248
1995
-
-
1
-
-
-
16
7
-
-
-
1
-
4
-
-
1
-
-
-
1
2
7
-
-
1
-
7
-
1
-
1
-
-
-
-
-
1
1
-
-
-
-
16
-
7
-
-
-
1
-
-
-
1
-
-
1
2
7
-
-
1
-
7
-
1
-
-
-
-
-
-
-
-
439343
Hutcheson
Regulation of 3-indoleacetic a ...
Pseudomonas savastanoi
J. Biol. Chem.
260
6281-6287
1985
-
-
-
-
-
1
2
1
-
-
2
1
-
4
-
-
1
-
-
-
1
-
1
1
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
1
-
2
-
1
-
-
2
1
-
-
-
1
-
-
1
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439344
Yamada
Nucleotide sequences of the Ps ...
Agrobacterium tumefaciens, Pseudomonas savastanoi
Proc. Natl. Acad. Sci. USA
82
6522-6526
1985
-
-
-
-
-
-
-
-
-
-
1
2
-
5
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
439345
Comai
Cloning characterization of ia ...
Pseudomonas savastanoi
J. Bacteriol.
149
40-46
1982
-
-
1
-
-
-
-
-
-
-
-
1
-
3
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
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Microbial synthesis and degrad ...
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