BRENDA - Enzyme Database show
show all sequences of 1.13.12.3

Aniline is an inducer, and not a precursor, for indole derivatives in Rubrivivax benzoatilyticus JA2

Mujahid, M.; Sasikala, C.h.; Ramana, C.h.V.; PLoS ONE 9, e87503 (2014)

Data extracted from this reference:

Cloned(Commentary)
Commentary
Organism
quantitative real time RT-PCR enzyme expression analysis
Rubrivivax benzoatilyticus
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-tryptophan + O2
Rubrivivax benzoatilyticus
-
(indol-3-yl)acetamide + CO2 + H2O
-
-
?
L-tryptophan + O2
Rubrivivax benzoatilyticus JA2
-
(indol-3-yl)acetamide + CO2 + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rubrivivax benzoatilyticus
-
-
-
Rubrivivax benzoatilyticus JA2
-
-
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-tryptophan + O2
-
746257
Rubrivivax benzoatilyticus
(indol-3-yl)acetamide + CO2 + H2O
-
-
-
?
L-tryptophan + O2
-
746257
Rubrivivax benzoatilyticus JA2
(indol-3-yl)acetamide + CO2 + H2O
-
-
-
?
Temperature Optimum [C]
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Rubrivivax benzoatilyticus
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
assay at
Rubrivivax benzoatilyticus
Cloned(Commentary) (protein specific)
Commentary
Organism
quantitative real time RT-PCR enzyme expression analysis
Rubrivivax benzoatilyticus
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
L-tryptophan + O2
Rubrivivax benzoatilyticus
-
(indol-3-yl)acetamide + CO2 + H2O
-
-
?
L-tryptophan + O2
Rubrivivax benzoatilyticus JA2
-
(indol-3-yl)acetamide + CO2 + H2O
-
-
?
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
L-tryptophan + O2
-
746257
Rubrivivax benzoatilyticus
(indol-3-yl)acetamide + CO2 + H2O
-
-
-
?
L-tryptophan + O2
-
746257
Rubrivivax benzoatilyticus JA2
(indol-3-yl)acetamide + CO2 + H2O
-
-
-
?
Temperature Optimum [C] (protein specific)
Temperature Optimum [C]
Temperature Optimum Maximum [C]
Commentary
Organism
37
-
assay at
Rubrivivax benzoatilyticus
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
assay at
Rubrivivax benzoatilyticus
Expression
Organism
Commentary
Expression
Rubrivivax benzoatilyticus
aniline (stress) stimulates tryptophan/indoles synthesis via the shikimate pathway, including tryptophan 2-monooxygenase activity, by possibly modulating the metabolic pathway
up
General Information
General Information
Commentary
Organism
metabolism
tryptophan aminotransferase (TAT) and tryptophan 2-monooxygenase (TMO) participate in first step of tryptophan catabolism leading to the formation of indole acetic acid. Indole biosynthesis in strain JA2 occurs via the de novo shikimate pathway. Induction of tryptophan aminotransferase and tryptophan 2-monooxygenase activities corroborates well with indole 3-acetic acid levels, suggesting that tryptophan catabolism occurs simultaneously in aniline exposed cells. Aniline (stress) stimulates tryptophan/indoles synthesis via the shikimate pathway by possibly modulating the metabolic pathway
Rubrivivax benzoatilyticus
physiological function
tryptophan 2-monooxygenase (TMO) participate in first step of tryptophan catabolism leading to the formation of indole acetic acid. Metabolic profiling reveals tryptophan accumulation only in aniline exposed cells along with indole 3-acetic acid and indole 3-aldehyde, the two major catabolites of tryptophan. Deuterium labelled aniline feeding studies reveal that while fumarate is the precursor of indole biosynthesis, aniline is not a precursor of indoles in strain JA2, but is an indole biosynthesis stimulator
Rubrivivax benzoatilyticus
General Information (protein specific)
General Information
Commentary
Organism
metabolism
tryptophan aminotransferase (TAT) and tryptophan 2-monooxygenase (TMO) participate in first step of tryptophan catabolism leading to the formation of indole acetic acid. Indole biosynthesis in strain JA2 occurs via the de novo shikimate pathway. Induction of tryptophan aminotransferase and tryptophan 2-monooxygenase activities corroborates well with indole 3-acetic acid levels, suggesting that tryptophan catabolism occurs simultaneously in aniline exposed cells. Aniline (stress) stimulates tryptophan/indoles synthesis via the shikimate pathway by possibly modulating the metabolic pathway
Rubrivivax benzoatilyticus
physiological function
tryptophan 2-monooxygenase (TMO) participate in first step of tryptophan catabolism leading to the formation of indole acetic acid. Metabolic profiling reveals tryptophan accumulation only in aniline exposed cells along with indole 3-acetic acid and indole 3-aldehyde, the two major catabolites of tryptophan. Deuterium labelled aniline feeding studies reveal that while fumarate is the precursor of indole biosynthesis, aniline is not a precursor of indoles in strain JA2, but is an indole biosynthesis stimulator
Rubrivivax benzoatilyticus
Expression (protein specific)
Organism
Commentary
Expression
Rubrivivax benzoatilyticus
aniline (stress) stimulates tryptophan/indoles synthesis via the shikimate pathway, including tryptophan 2-monooxygenase activity, by possibly modulating the metabolic pathway
up
Other publictions for EC 1.13.12.3
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [C]
Temperature Range [C]
Temperature Stability [C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [C] (protein specific)
Temperature Range [C] (protein specific)
Temperature Stability [C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
746273
Zhang
Regulation of oncogene expres ...
Agrobacterium tumefaciens, Agrobacterium tumefaciens C58
PLoS Pathog.
11
1-27
2015
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745813
Yin
Characterization of a tryptop ...
Puccinia graminis f. sp. tritici, Puccinia graminis f. sp. tritici CRL 75-36-700-3
Mol. Plant Microbe Interact.
27
227-235
2014
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10
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2
2
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746257
Mujahid
Aniline is an inducer, and no ...
Rubrivivax benzoatilyticus, Rubrivivax benzoatilyticus JA2
PLoS ONE
9
e87503
2014
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1
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1
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2
1
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727017
Gaweska
Structure of the flavoprotein ...
Pseudomonas savastanoi
Biochemistry
52
2620-2626
2013
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1
1
3
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5
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3
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1
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5
5
726752
Mujahid
Production of indole-3-acetic ...
Rubrivivax benzoatilyticus
Appl. Microbiol. Biotechnol.
89
1001-1008
2011
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3
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1
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672116
Ralph
Mechanistic studies of the fla ...
Pseudomonas savastanoi
Biochemistry
45
15844-15852
2006
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654682
Sobrado
Analysis of the role of the ac ...
Pseudomonas savastanoi
Biochemistry
42
13826-13832
2003
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2
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2
6
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1
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6
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6
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654683
Sobrado
Identification of Tyr413 as an ...
Pseudomonas savastanoi
Biochemistry
42
13833-13838
2003
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2
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2
6
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439346
Sobrado
Analysis of the roles of amino ...
Pseudomonas savastanoi
Arch. Biochem. Biophys.
402
24-30
2002
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3
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439349
Lemcke
The ORF8 gene product of Agrob ...
Agrobacterium rhizogenes
Mol. Plant Microbe Interact.
13
787-790
2000
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1
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4
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439348
Gadda
Characterization of 2-oxo-3-pe ...
Pseudomonas savastanoi
Biochemistry
38
5822-5828
1999
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1
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1
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439347
Emanuele
Purification and characterizat ...
Pseudomonas savastanoi
Arch. Biochem. Biophys.
316
241-248
1995
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1
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16
7
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7
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16
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7
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439343
Hutcheson
Regulation of 3-indoleacetic a ...
Pseudomonas savastanoi
J. Biol. Chem.
260
6281-6287
1985
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439344
Yamada
Nucleotide sequences of the Ps ...
Agrobacterium tumefaciens, Pseudomonas savastanoi
Proc. Natl. Acad. Sci. USA
82
6522-6526
1985
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439345
Comai
Cloning characterization of ia ...
Pseudomonas savastanoi
J. Bacteriol.
149
40-46
1982
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439342
Comai
Involvement of plasmid deoxyri ...
Pseudomonas savastanoi
J. Bacteriol.
143
950-957
1980
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439341
Kosuge
Microbial synthesis and degrad ...
Pseudomonas savastanoi
J. Biol. Chem.
241
3738-3744
1966
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19
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