Inhibitors | Comment | Organism | Structure |
---|---|---|---|
3,4-Dihydroxyphenylacetate | - |
Helianthus tuberosus | |
3,4-Dihydroxyphenylacetate | - |
Persea americana | |
3,4-Dihydroxyphenylacetate | - |
Ulva intestinalis | |
3,4-Dihydroxyphenylacetate | - |
Volvox carteri | |
3,4-Dihydroxyphenylpropionate | - |
Helianthus tuberosus | |
3,4-Dihydroxyphenylpropionate | - |
Persea americana | |
3,4-Dihydroxyphenylpropionate | - |
Ulva intestinalis | |
3,4-Dihydroxyphenylpropionate | - |
Volvox carteri | |
Pyridine 2,4-dicarboxylate | - |
Volvox carteri | |
Pyridine 2,5-dicarboxylate | - |
Volvox carteri |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.01 | - |
poly(L-proline) | - |
Volvox carteri | |
0.17 | - |
2-oxoglutarate | - |
Volvox carteri |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | - |
Helianthus tuberosus | |
Fe2+ | - |
Persea americana | |
Fe2+ | - |
Ulva intestinalis | |
Fe2+ | Km: 0.02 mM | Volvox carteri |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
40000 | - |
gel filtration | Ulva intestinalis |
40000 | - |
gel filtration | Volvox carteri |
65000 | - |
1 * 65000, SDS-PAGE | Volvox carteri |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Helianthus tuberosus | - |
- |
- |
Persea americana | - |
- |
- |
Ulva intestinalis | - |
- |
- |
Volvox carteri | - |
green algae | - |
Purification (Comment) | Organism |
---|---|
using ion-exchange chromatography on DEAE-cellulose, and affinity chromatography on poly(L-hydroxyproline), 3,4-dihydroxyphenylacetate or 3,4-dihydroxyphenylpropionate linked to Sepharose | Volvox carteri |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
additional information | - |
- |
Helianthus tuberosus |
additional information | - |
- |
Persea americana |
additional information | - |
- |
Ulva intestinalis |
additional information | - |
- |
Volvox carteri |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
additional information | proline or the dipeptides Ser-Pro or Ala-Pro do not serve as substrates | Volvox carteri | ? | - |
? | |
poly(L-Pro) + 2-oxoglutarate + O2 | - |
Helianthus tuberosus | poly(4-hydroxyproline) + succinate + CO2 | - |
? | |
poly(L-Pro) + 2-oxoglutarate + O2 | - |
Persea americana | poly(4-hydroxyproline) + succinate + CO2 | - |
? | |
poly(L-Pro) + 2-oxoglutarate + O2 | - |
Ulva intestinalis | poly(4-hydroxyproline) + succinate + CO2 | - |
? | |
poly(L-Pro) + 2-oxoglutarate + O2 | - |
Volvox carteri | poly(4-hydroxyproline) + succinate + CO2 | - |
? | |
poly(L-proline) + 2-oxoglutarate + O2 | - |
Volvox carteri | poly(trans-4-hydroxy-L-proline) + succinate + CO2 | - |
r |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 65000, SDS-PAGE | Volvox carteri |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
40 | - |
- |
Volvox carteri |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
6.6 | 6.8 | - |
Volvox carteri |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
ascorbate | - |
Helianthus tuberosus | |
ascorbate | - |
Persea americana | |
ascorbate | - |
Ulva intestinalis | |
ascorbate | Km: 0.14 mM | Volvox carteri |
Ki Value [mM] | Ki Value maximum [mM] | Inhibitor | Comment | Organism | Structure |
---|---|---|---|---|---|
0.008 | - |
Pyridine 2,4-dicarboxylate | - |
Volvox carteri | |
0.02 | - |
Pyridine 2,5-dicarboxylate | - |
Volvox carteri | |
0.024 | - |
3,4-Dihydroxyphenylpropionate | - |
Volvox carteri | |
0.04 | - |
3,4-Dihydroxyphenylacetate | - |
Volvox carteri | |
3 | - |
poly(L-hydroxyproline) | - |
Volvox carteri |