BRENDA - Enzyme Database show
show all sequences of 1.14.11.29

Characterization of the human prolyl 4-hydroxylases that modify the hypoxia-inducible factor

Hirsila, M.; Koivunen, P.; Gunzler, V.; Kivirikko, K.I.; Myllyharju, J.; J. Biol. Chem. 278, 30772-30780 (2003)

Data extracted from this reference:

Activating Compound
Activating Compound
Commentary
Organism
Structure
ascorbate
activates, Km: 0.14 mM; activates, Km: 0.17 mM; activates, Km: 0.18 mM
Homo sapiens
Cloned(Commentary)
Commentary
Organism
expression in Spodoptera frugiperda Sf9 cells and Escherichia coli; expression in Spodoptera frugiperda Sf9 cells and Escherichia coli; expression in Spodoptera frugiperda Sf9 cells and Escherichia coli
Homo sapiens
Engineering
Amino acid exchange
Commentary
Organism
R367K
inactive mutant of HIF-P4H-1
Homo sapiens
Inhibitors
Inhibitors
Commentary
Organism
Structure
3-carboxy-4-oxo-3,4-dihydro-1,10-phenanthroline
-
Homo sapiens
3-hydroxypyridine-2-carbonyl-glycine
-
Homo sapiens
N-((3-hydroxy-6-chloroquinolin-2-yl)carbonyl)glycine
-
Homo sapiens
oxalylglycine
-
Homo sapiens
Pyridine-2,4-dicarboxylate
-
Homo sapiens
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.006
-
DALDLEMLAPYISMDDDFQL
pH 7.8, 37°C
Homo sapiens
0.006
-
DLDLEMLAPYIPMDDDFQLRSFDQ
pH 7.8, 37°C; pH 7.8, 37°C
Homo sapiens
0.007
-
DALDLEMLAPYISMDDDFQL
pH 7.8, 37°C
Homo sapiens
0.007
-
DLDLEMLAPYIPMDDDF
pH 7.8, 37°C
Homo sapiens
0.007
-
DLDLEMLAPYIPMDDDFQL
pH 7.8, 37°C; pH 7.8, 37°C
Homo sapiens
0.007
-
DLEMLAPYIPMDDDFQL
pH 7.8, 37°C; pH 7.8, 37°C
Homo sapiens
0.007
-
EMLAPYIPMDDDFQL
pH 7.8, 37°C
Homo sapiens
0.008
-
DLDLEMLAPYIPMDDDFQL
pH 7.8, 37°C
Homo sapiens
0.008
-
DLDLEMLAPYIPMDDDFQLRSFDQ
pH 7.8, 37°C
Homo sapiens
0.009
-
DLDLEMLAPYIPMDD
pH 7.8, 37°C
Homo sapiens
0.01
-
DLDLEMLAPAIPMDDDFQL
pH 7.8, 37°C; pH 7.8, 37°C
Homo sapiens
0.011
-
ELDLETLAPYIPMDGEDFQ
pH 7.8, 37°C
Homo sapiens
0.014
-
DLEMLAPYIPMDDDFQL
pH 7.8, 37°C
Homo sapiens
0.015
-
DALDLEMLAPYISMDDDFQL
pH 7.8, 37°C
Homo sapiens
0.015
-
LAPYIPMDDDFQL
pH 7.8, 37°C
Homo sapiens
0.02
-
DLDLEMLAPAIPMDDDFQL
pH 7.8, 37°C
Homo sapiens
0.02
-
DLDLEMLAPGIPMDDDFQL
pH 7.8, 37°C; pH 7.8, 37°C; pH 7.8, 37°C
Homo sapiens
0.03
-
DLDLEMLAPYIPMD
pH 7.8, 37°C
Homo sapiens
0.03
-
ELDLETLAPYIPMDGEDFQ
pH 7.8, 37°C; pH 7.8, 37°C
Homo sapiens
0.035
-
EMLAPYIPMDDDFQL
pH 7.8, 37°C
Homo sapiens
0.05
-
DLDLEMLAPYIPMDDDF
pH 7.8, 37°C
Homo sapiens
0.05
-
EMLAPYIPMDD
pH 7.8, 37°C
Homo sapiens
0.055
-
2-oxoglutarate
pH 7.8, 37°C
Homo sapiens
0.06
-
2-oxoglutarate
pH 7.8, 37°C; pH 7.8, 37°C
Homo sapiens
0.06
-
DLDLEMLAPYIPMDD
pH 7.8, 37°C
Homo sapiens
0.06
-
EPEELAQLAPTPGDAIISLD
pH 7.8, 37°C
Homo sapiens
0.07
-
DLDLEMLAPYIPMDDDF
pH 7.8, 37°C
Homo sapiens
0.07
-
EEPDLSCLAPFVDTYDMMQM
pH 7.8, 37°C
Homo sapiens
0.08
-
EMLAPYIPMDDDFQL
pH 7.8, 37°C
Homo sapiens
0.1
-
DLDLEMLAPYIPMD
pH 7.8, 37°C; pH 7.8, 37°C
Homo sapiens
0.1
-
DLDLEMLAPYIPMDD
pH 7.8, 37°C
Homo sapiens
0.1
-
EEPDLSCLAPFVDTYDMMQM
pH 7.8, 37°C
Homo sapiens
0.1
-
EPEELAQLAPTPGDAIISLD
pH 7.8, 37°C; pH 7.8, 37°C
Homo sapiens
0.13
-
DALTLLAPAAGDTIISLFG
pH 7.8, 37°C
Homo sapiens
0.23
-
O2
pH 7.8, 37°C; pH 7.8, 37°C
Homo sapiens
0.25
-
O2
pH 7.8, 37°C
Homo sapiens
0.38
-
DALTLLAPAAGDTIISLFG
pH 7.8, 37°C
Homo sapiens
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
hypoxia-inducible factor-L-proline + 2-oxoglutarate + O2
Homo sapiens
-
hypoxia-inducible factor-(4R)-4-hydroxy-L-proline + succinate + CO2
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Homo sapiens
-
-
-
Homo sapiens
Q9GZT9
HIF-P4H-2
-
Homo sapiens
Q9H6Z9
HIF-P4H-3
-
Purification (Commentary)
Commentary
Organism
;
Homo sapiens
Source Tissue
Source Tissue
Commentary
Organism
Textmining
brain
expression level of of HIF-P4H-3 mRNA is highest in the adult heart, brain, placenta, lung, and skeletal muscle and in the fetal heart, spleen, and skeletal muscle; the level of HIF-P4H-1 mRNA expression is highest in the adult brain, placenta, lung, and kidney; the levels of HIF-P4H-2 mRNA expression is highest in the adult heart, brain, lung, and liver and in the fetal brain, heart, spleen, and skeletal muscle
Homo sapiens
-
heart
expression level of of HIF-P4H-3 mRNA is highest in the adult heart, brain, placenta, lung, and skeletal muscle and in the fetal heart, spleen, and skeletal muscle; the levels of HIF-P4H-2 mRNA expression is highest in the adult heart, brain, lung, and liver and in the fetal brain, heart, spleen, and skeletal muscle
Homo sapiens
-
kidney
the level of HIF-P4H-1 mRNA expression is highest in the adult brain, placenta, lung, and kidney
Homo sapiens
-
liver
the levels of HIF-P4H-2 mRNA expression is highest in the adult heart, brain, lung, and liver and in the fetal brain, heart, spleen, and skeletal muscle
Homo sapiens
-
lung
expression level of of HIF-P4H-3 mRNA is highest in the adult heart, brain, placenta, lung, and skeletal muscle and in the fetal heart, spleen, and skeletal muscle; the level of HIF-P4H-1 mRNA expression is highest in the adult brain, placenta, lung, and kidney; the levels of HIF-P4H-2 mRNA expression is highest in the adult heart, brain, lung, and liver and in the fetal brain, heart, spleen, and skeletal muscle
Homo sapiens
-
placenta
expression level of of HIF-P4H-3 mRNA is highest in the adult heart, brain, placenta, lung, and skeletal muscle and in the fetal heart, spleen, and skeletal muscle; the level of HIF-P4H-1 mRNA expression is highest in the adult brain, placenta, lung, and kidney
Homo sapiens
-
skeletal muscle
expression level of of HIF-P4H-3 mRNA is highest in the adult heart, brain, placenta, lung, and skeletal muscle and in the fetal heart, spleen, and skeletal muscle; the levels of HIF-P4H-2 mRNA expression is highest in the adult heart, brain, lung, and liver and in the fetal brain, heart, spleen, and skeletal muscle
Homo sapiens
-
spleen
expression level of of HIF-P4H-3 mRNA is highest in the adult heart, brain, placenta, lung, and skeletal muscle and in the fetal heart, spleen, and skeletal muscle; the levels of HIF-P4H-2 mRNA expression is highest in the adult heart, brain, lung, and liver and in the fetal brain, heart, spleen, and skeletal muscle
Homo sapiens
-
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
DALDLEMLAPYISMDDDFQL + 2-oxoglutarate + O2
a HIF-3alpha peptide. Vmax is 120% of the activity with DLDLEMLAPYIPMDDDFQL
659235
Homo sapiens
?
-
-
-
?
DALDLEMLAPYISMDDDFQL + 2-oxoglutarate + O2
a HIF-3alpha peptide. Vmax is 120% of the activity with DLDLEMLAPYIPMDDDFQL
659235
Homo sapiens
DALDLEMLA-((4R)-4-hydroxy-L-proline)-YISMDDDFQL + succinate + CO2
-
-
-
?
DALDLEMLAPYISMDDDFQL + 2-oxoglutarate + O2
a HIF-3alpha peptide. Vmax is 150% of the activity with DLDLEMLAPYIPMDDDFQL
659235
Homo sapiens
DALDLEMLA-((4R)-4-hydroxy-L-proline)-YISMDDDFQL + succinate + CO2
-
-
-
?
DALTLLAPAAGDTIISLFG + 2-oxoglutarate + O2
N-terminal hydroxylation site of HIF-1alpha, Vmax is 100% of the activity with DLDLEMLAPYIPMDDDFQL
659235
Homo sapiens
DALTLLA-((4R)-4-hydroxy-L-proline)-AAGDTIISLFG + succinate + CO2
-
-
-
?
DALTLLAPAAGDTIISLFG + 2-oxoglutarate + O2
N-terminal hydroxylation site of HIF-1alpha, Vmax is 60% of the activity with DLDLEMLAPYIPMDDDFQL
659235
Homo sapiens
DALTLLA-((4R)-4-hydroxy-L-proline)-AAGDTIISLFG + succinate + CO2
-
-
-
?
DLDLEMLAPAIPMDDDFQL + 2-oxoglutarate + O2
Vmax is 100% of the activity with DLDLEMLAPYIPMDDDFQL
659235
Homo sapiens
DLDLEMLA-((4R)-4-hydroxy-L-proline)-AIPMDDDFQL + succinate + CO2
-
-
-
?
DLDLEMLAPAIPMDDDFQL + 2-oxoglutarate + O2
Vmax is 100% of the activity with DLDLEMLAPYIPMDDDFQL
659235
Homo sapiens
DLDLEMLA-((4R)-4-hydroxy-L-proline)-AIPMDDDFQL succinate + CO2
-
-
-
?
DLDLEMLAPGIPMDDDFQL + 2-oxoglutarate + O2
Vmax is 100% of the activity with DLDLEMLAPYIPMDDDFQL
659235
Homo sapiens
DLDLEMLA-((4R)-4-hydroxy-L-proline)-GIPMDDDFQL + succinate + CO2
-
-
-
?
DLDLEMLAPYIPMD + 2-oxoglutarate + O2
Vmax is 100% of the activity with DLDLEMLAPYIPMDDDFQL
659235
Homo sapiens
DLDLEMLA-((4R)-4-hydroxy-L-proline)-YIPMD + succinate + CO2
-
-
-
?
DLDLEMLAPYIPMDD + 2-oxoglutarate + O2
Vmax is 100% of the activity with DLDLEMLAPYIPMDDDFQL
659235
Homo sapiens
DLDLEMLA-((4R)-4-hydroxy-L-proline)-YIPMDD + succinate + CO2
-
-
-
?
DLDLEMLAPYIPMDDDF + 2-oxoglutarate + O2
Vmax is 100% of the activity with DLDLEMLAPYIPMDDDFQL
659235
Homo sapiens
DLDLEMLA-((4R)-4-hydroxy-L-proline)-YIPMDDDF + succinate + CO2
-
-
-
?
DLDLEMLAPYIPMDDDFQL + 2-oxoglutarate + O2
-
659235
Homo sapiens
DLDLEMLA-((4R)-4-hydroxy-L-proline)-YIPMDDDFQL + succinate + CO2
-
-
-
?
DLDLEMLAPYIPMDDDFQLRSFDQ + 2-oxoglutarate + O2
Vmax is 100% of the activity with DLDLEMLAPYIPMDDDFQL
659235
Homo sapiens
DLDLEMLA-((4R)-4-hydroxy-L-proline)-YIPMDDDFQLRSFDQ + succinate + CO2
-
-
-
?
DLEMLAPYIPMDDDFQL + 2-oxoglutarate + O2
Vmax is 100% of the activity with DLDLEMLAPYIPMDDDFQL
659235
Homo sapiens
DLDLEMLA-((4R)-4-hydroxy-L-proline)-YIPMDDDFQL + succinate + CO2
-
-
-
?
DLEMLAPYIPMDDDFQL + 2-oxoglutarate + O2
Vmax is 100% of the activity with DLDLEMLAPYIPMDDDFQL
659235
Homo sapiens
DLEMLA-((4R)-4-hydroxy-L-proline)-YIPMDDDFQL + succinate + CO2
-
-
-
?
EEPDLSCLAPFVDTYDMMQM + 2-oxoglutarate + O2
hydroxylation site of Caenorhabditis elegans HIF-alpha. Vmax is 60% of the activity with DLDLEMLAPYIPMDDDFQL
659235
Homo sapiens
?
-
-
-
?
EEPDLSCLAPFVDTYDMMQM + 2-oxoglutarate + O2
hydroxylation site of Caenorhabditis elegans HIF-alpha. Vmax is 80% of the activity with DLDLEMLAPYIPMDDDFQL
659235
Homo sapiens
?
-
-
-
?
ELDLETLAPYIPMDGEDFQ + 2-oxoglutarate + O2
C-terminal hydroxylation site of HIF-2alpha. Vmax is 100% of the activity with DLDLEMLAPYIPMDDDFQL
659235
Homo sapiens
?
-
-
-
?
ELDLETLAPYIPMDGEDFQ + 2-oxoglutarate + O2
C-terminal hydroxylation site of HIF-2alpha. Vmax is 70% of the activity with DLDLEMLAPYIPMDDDFQL
659235
Homo sapiens
?
-
-
-
?
ELDLETLAPYIPMDGEDFQ + 2-oxoglutarate + O2
C-terminal hydroxylation site of HIF-2alpha. Vmax is 80% of the activity with DLDLEMLAPYIPMDDDFQL
659235
Homo sapiens
ELDLETLA-((4R)-4-hydroxy-L-proline)-YIPMDGEDFQ + succinate + CO2
-
-
-
?
EMLAPYIPMDD + 2-oxoglutarate + O2
Vmax is 30% of the activity with DLDLEMLAPYIPMDDDFQL
659235
Homo sapiens
EMLA-((4R)-4-hydroxy-L-proline)-YIPMDD + succinate + CO2
-
-
-
?
EMLAPYIPMDDDFQL + 2-oxoglutarate + O2
Vmax is 100% of the activity with DLDLEMLAPYIPMDDDFQL
659235
Homo sapiens
EMLA-((4R)-4-hydroxy-L-proline)-YIPMDDDFQL + succinate + CO2
-
-
-
?
EMLAPYIPMDDDFQL + 2-oxoglutarate + O2
Vmax is 80% of the activity with DLDLEMLAPYIPMDDDFQL
659235
Homo sapiens
EMLA-((4R)-4-hydroxy-L-proline)-YIPMDDDFQL + succinate + CO2
-
-
-
?
EPEELAQLAPTPGDAIISLD + 2-oxoglutarate + O2
N-terminal hydroxylation site of HIF-2alpha. Vmax is 30% of the activity with DLDLEMLAPYIPMDDDFQL
659235
Homo sapiens
?
-
-
-
?
EPEELAQLAPTPGDAIISLD + 2-oxoglutarate + O2
N-terminal hydroxylation site of HIF-2alpha. Vmax is 70% of the activity with DLDLEMLAPYIPMDDDFQL
659235
Homo sapiens
?
-
-
-
?
EPEELAQLAPTPGDAIISLD + 2-oxoglutarate + O2
N-terminal hydroxylation site of HIF-2alpha. Vmax is 80% of the activity with DLDLEMLAPYIPMDDDFQL
659235
Homo sapiens
?
-
-
-
?
hypoxia-inducible factor-L-proline + 2-oxoglutarate + O2
-
659235
Homo sapiens
hypoxia-inducible factor-(4R)-4-hydroxy-L-proline + succinate + CO2
-
-
-
?
LAPYIPMDDDFQL + 2-oxoglutarate + O2
Vmax is 90% of the activity with DLDLEMLAPYIPMDDDFQL
659235
Homo sapiens
LA-((4R)-4-hydroxy-L-proline)-YIPMDDDFQL + succinate + CO2
-
-
-
?
additional information
the enzyme requires long peptide substrates. No hydroxylation of: Leu-Ala-Pro, Leu-Ala-Pro-Tyr, Leu-Glu-Met-Leu-Ala-Pro, and Leu-Glu-Met-Leu-Ala-Pro-Tyr
659235
Homo sapiens
?
-
-
-
-
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at; assay at; assay at
Homo sapiens
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
assay at; assay at; assay at
Homo sapiens
Ki Value [mM]
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
additional information
-
additional information
Ki-values above 0.3 mM: pyridine-2,5-dicarboxylate and 3,4-dihydroxybenzoic acid; Ki-values above 0.3 mM: pyridine-2,5-dicarboxylate and 3,4-dihydroxybenzoic acid; Ki-values above 0.3 mM: pyridine-2,5-dicarboxylate and 3,4-dihydroxybenzoic acid
Homo sapiens
0.0002
-
N-((3-hydroxy-6-chloroquinolin-2-yl)carbonyl)glycine
pH 7.8, 37°C; pH 7.8, 37°C
Homo sapiens
0.0008
-
N-((3-hydroxy-6-chloroquinolin-2-yl)carbonyl)glycine
pH 7.8, 37°C
Homo sapiens
0.001
-
3-hydroxypyridine-2-carbonyl-glycine
pH 7.8, 37°C
Homo sapiens
0.002
-
3-hydroxypyridine-2-carbonyl-glycine
pH 7.8, 37°C
Homo sapiens
0.007
-
Pyridine-2,4-dicarboxylate
pH 7.8, 37°C
Homo sapiens
0.008
-
oxalylglycine
pH 7.8, 37°C
Homo sapiens
0.008
-
Pyridine-2,4-dicarboxylate
pH 7.8, 37°C
Homo sapiens
0.01
-
3-carboxy-4-oxo-3,4-dihydro-1,10-phenanthroline
pH 7.8, 37°C; pH 7.8, 37°C
Homo sapiens
0.01
-
oxalylglycine
pH 7.8, 37°C
Homo sapiens
0.015
-
3-hydroxypyridine-2-carbonyl-glycine
pH 7.8, 37°C
Homo sapiens
0.03
-
3-carboxy-4-oxo-3,4-dihydro-1,10-phenanthroline
pH 7.8, 37°C
Homo sapiens
0.04
-
Pyridine-2,4-dicarboxylate
pH 7.8, 37°C
Homo sapiens
0.05
-
oxalylglycine
pH 7.8, 37°C
Homo sapiens
Activating Compound (protein specific)
Activating Compound
Commentary
Organism
Structure
ascorbate
activates, Km: 0.17 mM
Homo sapiens
ascorbate
activates, Km: 0.14 mM
Homo sapiens
ascorbate
activates, Km: 0.18 mM
Homo sapiens
Cloned(Commentary) (protein specific)
Commentary
Organism
expression in Spodoptera frugiperda Sf9 cells and Escherichia coli
Homo sapiens
Engineering (protein specific)
Amino acid exchange
Commentary
Organism
R367K
inactive mutant of HIF-P4H-1
Homo sapiens
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
3-carboxy-4-oxo-3,4-dihydro-1,10-phenanthroline
-
Homo sapiens
3-hydroxypyridine-2-carbonyl-glycine
-
Homo sapiens
N-((3-hydroxy-6-chloroquinolin-2-yl)carbonyl)glycine
-
Homo sapiens
oxalylglycine
-
Homo sapiens
Pyridine-2,4-dicarboxylate
-
Homo sapiens
Ki Value [mM] (protein specific)
Ki Value [mM]
Ki Value maximum [mM]
Inhibitor
Commentary
Organism
Structure
additional information
-
additional information
Ki-values above 0.3 mM: pyridine-2,5-dicarboxylate and 3,4-dihydroxybenzoic acid
Homo sapiens
0.0002
-
N-((3-hydroxy-6-chloroquinolin-2-yl)carbonyl)glycine
pH 7.8, 37°C
Homo sapiens
0.0008
-
N-((3-hydroxy-6-chloroquinolin-2-yl)carbonyl)glycine
pH 7.8, 37°C
Homo sapiens
0.001
-
3-hydroxypyridine-2-carbonyl-glycine
pH 7.8, 37°C
Homo sapiens
0.002
-
3-hydroxypyridine-2-carbonyl-glycine
pH 7.8, 37°C
Homo sapiens
0.007
-
Pyridine-2,4-dicarboxylate
pH 7.8, 37°C
Homo sapiens
0.008
-
oxalylglycine
pH 7.8, 37°C
Homo sapiens
0.008
-
Pyridine-2,4-dicarboxylate
pH 7.8, 37°C
Homo sapiens
0.01
-
3-carboxy-4-oxo-3,4-dihydro-1,10-phenanthroline
pH 7.8, 37°C
Homo sapiens
0.01
-
oxalylglycine
pH 7.8, 37°C
Homo sapiens
0.015
-
3-hydroxypyridine-2-carbonyl-glycine
pH 7.8, 37°C
Homo sapiens
0.03
-
3-carboxy-4-oxo-3,4-dihydro-1,10-phenanthroline
pH 7.8, 37°C
Homo sapiens
0.04
-
Pyridine-2,4-dicarboxylate
pH 7.8, 37°C
Homo sapiens
0.05
-
oxalylglycine
pH 7.8, 37°C
Homo sapiens
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.006
-
DALDLEMLAPYISMDDDFQL
pH 7.8, 37°C
Homo sapiens
0.006
-
DLDLEMLAPYIPMDDDFQLRSFDQ
pH 7.8, 37°C
Homo sapiens
0.007
-
DALDLEMLAPYISMDDDFQL
pH 7.8, 37°C
Homo sapiens
0.007
-
DLDLEMLAPYIPMDDDF
pH 7.8, 37°C
Homo sapiens
0.007
-
DLDLEMLAPYIPMDDDFQL
pH 7.8, 37°C
Homo sapiens
0.007
-
DLEMLAPYIPMDDDFQL
pH 7.8, 37°C
Homo sapiens
0.007
-
EMLAPYIPMDDDFQL
pH 7.8, 37°C
Homo sapiens
0.008
-
DLDLEMLAPYIPMDDDFQL
pH 7.8, 37°C
Homo sapiens
0.008
-
DLDLEMLAPYIPMDDDFQLRSFDQ
pH 7.8, 37°C
Homo sapiens
0.009
-
DLDLEMLAPYIPMDD
pH 7.8, 37°C
Homo sapiens
0.01
-
DLDLEMLAPAIPMDDDFQL
pH 7.8, 37°C
Homo sapiens
0.011
-
ELDLETLAPYIPMDGEDFQ
pH 7.8, 37°C
Homo sapiens
0.014
-
DLEMLAPYIPMDDDFQL
pH 7.8, 37°C
Homo sapiens
0.015
-
DALDLEMLAPYISMDDDFQL
pH 7.8, 37°C
Homo sapiens
0.015
-
LAPYIPMDDDFQL
pH 7.8, 37°C
Homo sapiens
0.02
-
DLDLEMLAPAIPMDDDFQL
pH 7.8, 37°C
Homo sapiens
0.02
-
DLDLEMLAPGIPMDDDFQL
pH 7.8, 37°C
Homo sapiens
0.03
-
DLDLEMLAPYIPMD
pH 7.8, 37°C
Homo sapiens
0.03
-
ELDLETLAPYIPMDGEDFQ
pH 7.8, 37°C
Homo sapiens
0.035
-
EMLAPYIPMDDDFQL
pH 7.8, 37°C
Homo sapiens
0.05
-
DLDLEMLAPYIPMDDDF
pH 7.8, 37°C
Homo sapiens
0.05
-
EMLAPYIPMDD
pH 7.8, 37°C
Homo sapiens
0.055
-
2-oxoglutarate
pH 7.8, 37°C
Homo sapiens
0.06
-
2-oxoglutarate
pH 7.8, 37°C
Homo sapiens
0.06
-
DLDLEMLAPYIPMDD
pH 7.8, 37°C
Homo sapiens
0.06
-
EPEELAQLAPTPGDAIISLD
pH 7.8, 37°C
Homo sapiens
0.07
-
DLDLEMLAPYIPMDDDF
pH 7.8, 37°C
Homo sapiens
0.07
-
EEPDLSCLAPFVDTYDMMQM
pH 7.8, 37°C
Homo sapiens
0.08
-
EMLAPYIPMDDDFQL
pH 7.8, 37°C
Homo sapiens
0.1
-
DLDLEMLAPYIPMD
pH 7.8, 37°C
Homo sapiens
0.1
-
DLDLEMLAPYIPMDD
pH 7.8, 37°C
Homo sapiens
0.1
-
EEPDLSCLAPFVDTYDMMQM
pH 7.8, 37°C
Homo sapiens
0.1
-
EPEELAQLAPTPGDAIISLD
pH 7.8, 37°C
Homo sapiens
0.13
-
DALTLLAPAAGDTIISLFG
pH 7.8, 37°C
Homo sapiens
0.23
-
O2
pH 7.8, 37°C
Homo sapiens
0.25
-
O2
pH 7.8, 37°C
Homo sapiens
0.38
-
DALTLLAPAAGDTIISLFG
pH 7.8, 37°C
Homo sapiens
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
hypoxia-inducible factor-L-proline + 2-oxoglutarate + O2
Homo sapiens
-
hypoxia-inducible factor-(4R)-4-hydroxy-L-proline + succinate + CO2
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Homo sapiens
Source Tissue (protein specific)
Source Tissue
Commentary
Organism
Textmining
brain
the level of HIF-P4H-1 mRNA expression is highest in the adult brain, placenta, lung, and kidney
Homo sapiens
-
brain
expression level of of HIF-P4H-3 mRNA is highest in the adult heart, brain, placenta, lung, and skeletal muscle and in the fetal heart, spleen, and skeletal muscle
Homo sapiens
-
brain
the levels of HIF-P4H-2 mRNA expression is highest in the adult heart, brain, lung, and liver and in the fetal brain, heart, spleen, and skeletal muscle
Homo sapiens
-
heart
expression level of of HIF-P4H-3 mRNA is highest in the adult heart, brain, placenta, lung, and skeletal muscle and in the fetal heart, spleen, and skeletal muscle
Homo sapiens
-
heart
the levels of HIF-P4H-2 mRNA expression is highest in the adult heart, brain, lung, and liver and in the fetal brain, heart, spleen, and skeletal muscle
Homo sapiens
-
kidney
the level of HIF-P4H-1 mRNA expression is highest in the adult brain, placenta, lung, and kidney
Homo sapiens
-
liver
the levels of HIF-P4H-2 mRNA expression is highest in the adult heart, brain, lung, and liver and in the fetal brain, heart, spleen, and skeletal muscle
Homo sapiens
-
lung
the level of HIF-P4H-1 mRNA expression is highest in the adult brain, placenta, lung, and kidney
Homo sapiens
-
lung
expression level of of HIF-P4H-3 mRNA is highest in the adult heart, brain, placenta, lung, and skeletal muscle and in the fetal heart, spleen, and skeletal muscle
Homo sapiens
-
lung
the levels of HIF-P4H-2 mRNA expression is highest in the adult heart, brain, lung, and liver and in the fetal brain, heart, spleen, and skeletal muscle
Homo sapiens
-
placenta
the level of HIF-P4H-1 mRNA expression is highest in the adult brain, placenta, lung, and kidney
Homo sapiens
-
placenta
expression level of of HIF-P4H-3 mRNA is highest in the adult heart, brain, placenta, lung, and skeletal muscle and in the fetal heart, spleen, and skeletal muscle
Homo sapiens
-
skeletal muscle
expression level of of HIF-P4H-3 mRNA is highest in the adult heart, brain, placenta, lung, and skeletal muscle and in the fetal heart, spleen, and skeletal muscle
Homo sapiens
-
skeletal muscle
the levels of HIF-P4H-2 mRNA expression is highest in the adult heart, brain, lung, and liver and in the fetal brain, heart, spleen, and skeletal muscle
Homo sapiens
-
spleen
expression level of of HIF-P4H-3 mRNA is highest in the adult heart, brain, placenta, lung, and skeletal muscle and in the fetal heart, spleen, and skeletal muscle
Homo sapiens
-
spleen
the levels of HIF-P4H-2 mRNA expression is highest in the adult heart, brain, lung, and liver and in the fetal brain, heart, spleen, and skeletal muscle
Homo sapiens
-
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
DALDLEMLAPYISMDDDFQL + 2-oxoglutarate + O2
a HIF-3alpha peptide. Vmax is 120% of the activity with DLDLEMLAPYIPMDDDFQL
659235
Homo sapiens
?
-
-
-
?
DALDLEMLAPYISMDDDFQL + 2-oxoglutarate + O2
a HIF-3alpha peptide. Vmax is 120% of the activity with DLDLEMLAPYIPMDDDFQL
659235
Homo sapiens
DALDLEMLA-((4R)-4-hydroxy-L-proline)-YISMDDDFQL + succinate + CO2
-
-
-
?
DALDLEMLAPYISMDDDFQL + 2-oxoglutarate + O2
a HIF-3alpha peptide. Vmax is 150% of the activity with DLDLEMLAPYIPMDDDFQL
659235
Homo sapiens
DALDLEMLA-((4R)-4-hydroxy-L-proline)-YISMDDDFQL + succinate + CO2
-
-
-
?
DALTLLAPAAGDTIISLFG + 2-oxoglutarate + O2
N-terminal hydroxylation site of HIF-1alpha, Vmax is 100% of the activity with DLDLEMLAPYIPMDDDFQL
659235
Homo sapiens
DALTLLA-((4R)-4-hydroxy-L-proline)-AAGDTIISLFG + succinate + CO2
-
-
-
?
DALTLLAPAAGDTIISLFG + 2-oxoglutarate + O2
N-terminal hydroxylation site of HIF-1alpha, Vmax is 60% of the activity with DLDLEMLAPYIPMDDDFQL
659235
Homo sapiens
DALTLLA-((4R)-4-hydroxy-L-proline)-AAGDTIISLFG + succinate + CO2
-
-
-
?
DLDLEMLAPAIPMDDDFQL + 2-oxoglutarate + O2
Vmax is 100% of the activity with DLDLEMLAPYIPMDDDFQL
659235
Homo sapiens
DLDLEMLA-((4R)-4-hydroxy-L-proline)-AIPMDDDFQL + succinate + CO2
-
-
-
?
DLDLEMLAPAIPMDDDFQL + 2-oxoglutarate + O2
Vmax is 100% of the activity with DLDLEMLAPYIPMDDDFQL
659235
Homo sapiens
DLDLEMLA-((4R)-4-hydroxy-L-proline)-AIPMDDDFQL succinate + CO2
-
-
-
?
DLDLEMLAPGIPMDDDFQL + 2-oxoglutarate + O2
Vmax is 100% of the activity with DLDLEMLAPYIPMDDDFQL
659235
Homo sapiens
DLDLEMLA-((4R)-4-hydroxy-L-proline)-GIPMDDDFQL + succinate + CO2
-
-
-
?
DLDLEMLAPYIPMD + 2-oxoglutarate + O2
Vmax is 100% of the activity with DLDLEMLAPYIPMDDDFQL
659235
Homo sapiens
DLDLEMLA-((4R)-4-hydroxy-L-proline)-YIPMD + succinate + CO2
-
-
-
?
DLDLEMLAPYIPMDD + 2-oxoglutarate + O2
Vmax is 100% of the activity with DLDLEMLAPYIPMDDDFQL
659235
Homo sapiens
DLDLEMLA-((4R)-4-hydroxy-L-proline)-YIPMDD + succinate + CO2
-
-
-
?
DLDLEMLAPYIPMDDDF + 2-oxoglutarate + O2
Vmax is 100% of the activity with DLDLEMLAPYIPMDDDFQL
659235
Homo sapiens
DLDLEMLA-((4R)-4-hydroxy-L-proline)-YIPMDDDF + succinate + CO2
-
-
-
?
DLDLEMLAPYIPMDDDFQL + 2-oxoglutarate + O2
-
659235
Homo sapiens
DLDLEMLA-((4R)-4-hydroxy-L-proline)-YIPMDDDFQL + succinate + CO2
-
-
-
?
DLDLEMLAPYIPMDDDFQLRSFDQ + 2-oxoglutarate + O2
Vmax is 100% of the activity with DLDLEMLAPYIPMDDDFQL
659235
Homo sapiens
DLDLEMLA-((4R)-4-hydroxy-L-proline)-YIPMDDDFQLRSFDQ + succinate + CO2
-
-
-
?
DLEMLAPYIPMDDDFQL + 2-oxoglutarate + O2
Vmax is 100% of the activity with DLDLEMLAPYIPMDDDFQL
659235
Homo sapiens
DLDLEMLA-((4R)-4-hydroxy-L-proline)-YIPMDDDFQL + succinate + CO2
-
-
-
?
DLEMLAPYIPMDDDFQL + 2-oxoglutarate + O2
Vmax is 100% of the activity with DLDLEMLAPYIPMDDDFQL
659235
Homo sapiens
DLEMLA-((4R)-4-hydroxy-L-proline)-YIPMDDDFQL + succinate + CO2
-
-
-
?
EEPDLSCLAPFVDTYDMMQM + 2-oxoglutarate + O2
hydroxylation site of Caenorhabditis elegans HIF-alpha. Vmax is 60% of the activity with DLDLEMLAPYIPMDDDFQL
659235
Homo sapiens
?
-
-
-
?
EEPDLSCLAPFVDTYDMMQM + 2-oxoglutarate + O2
hydroxylation site of Caenorhabditis elegans HIF-alpha. Vmax is 80% of the activity with DLDLEMLAPYIPMDDDFQL
659235
Homo sapiens
?
-
-
-
?
ELDLETLAPYIPMDGEDFQ + 2-oxoglutarate + O2
C-terminal hydroxylation site of HIF-2alpha. Vmax is 100% of the activity with DLDLEMLAPYIPMDDDFQL
659235
Homo sapiens
?
-
-
-
?
ELDLETLAPYIPMDGEDFQ + 2-oxoglutarate + O2
C-terminal hydroxylation site of HIF-2alpha. Vmax is 70% of the activity with DLDLEMLAPYIPMDDDFQL
659235
Homo sapiens
?
-
-
-
?
ELDLETLAPYIPMDGEDFQ + 2-oxoglutarate + O2
C-terminal hydroxylation site of HIF-2alpha. Vmax is 80% of the activity with DLDLEMLAPYIPMDDDFQL
659235
Homo sapiens
ELDLETLA-((4R)-4-hydroxy-L-proline)-YIPMDGEDFQ + succinate + CO2
-
-
-
?
EMLAPYIPMDD + 2-oxoglutarate + O2
Vmax is 30% of the activity with DLDLEMLAPYIPMDDDFQL
659235
Homo sapiens
EMLA-((4R)-4-hydroxy-L-proline)-YIPMDD + succinate + CO2
-
-
-
?
EMLAPYIPMDDDFQL + 2-oxoglutarate + O2
Vmax is 100% of the activity with DLDLEMLAPYIPMDDDFQL
659235
Homo sapiens
EMLA-((4R)-4-hydroxy-L-proline)-YIPMDDDFQL + succinate + CO2
-
-
-
?
EMLAPYIPMDDDFQL + 2-oxoglutarate + O2
Vmax is 80% of the activity with DLDLEMLAPYIPMDDDFQL
659235
Homo sapiens
EMLA-((4R)-4-hydroxy-L-proline)-YIPMDDDFQL + succinate + CO2
-
-
-
?
EPEELAQLAPTPGDAIISLD + 2-oxoglutarate + O2
N-terminal hydroxylation site of HIF-2alpha. Vmax is 30% of the activity with DLDLEMLAPYIPMDDDFQL
659235
Homo sapiens
?
-
-
-
?
EPEELAQLAPTPGDAIISLD + 2-oxoglutarate + O2
N-terminal hydroxylation site of HIF-2alpha. Vmax is 70% of the activity with DLDLEMLAPYIPMDDDFQL
659235
Homo sapiens
?
-
-
-
?
EPEELAQLAPTPGDAIISLD + 2-oxoglutarate + O2
N-terminal hydroxylation site of HIF-2alpha. Vmax is 80% of the activity with DLDLEMLAPYIPMDDDFQL
659235
Homo sapiens
?
-
-
-
?
hypoxia-inducible factor-L-proline + 2-oxoglutarate + O2
-
659235
Homo sapiens
hypoxia-inducible factor-(4R)-4-hydroxy-L-proline + succinate + CO2
-
-
-
?
LAPYIPMDDDFQL + 2-oxoglutarate + O2
Vmax is 90% of the activity with DLDLEMLAPYIPMDDDFQL
659235
Homo sapiens
LA-((4R)-4-hydroxy-L-proline)-YIPMDDDFQL + succinate + CO2
-
-
-
?
additional information
the enzyme requires long peptide substrates. No hydroxylation of: Leu-Ala-Pro, Leu-Ala-Pro-Tyr, Leu-Glu-Met-Leu-Ala-Pro, and Leu-Glu-Met-Leu-Ala-Pro-Tyr
659235
Homo sapiens
?
-
-
-
-
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
37
-
assay at
Homo sapiens
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.8
-
assay at
Homo sapiens
Other publictions for EC 1.14.11.29
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
741945
Osipyants
Enzyme–substrate reporters ...
Homo sapiens
Biochemistry
82
1207-1214
2017
-
-
-
-
-
-
4
-
-
-
-
3
-
3
-
-
-
-
-
1
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
12
-
-
-
-
-
3
-
-
-
-
-
3
-
-
3
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
742225
Miikkulainen
HIF prolyl hydroxylase PHD3 r ...
Homo sapiens
Cancer Metab.
5
5-5
2017
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
742585
Badawi
Relative contribution of prol ...
Homo sapiens
Front. Neurosci.
11
239
2017
-
-
-
-
-
-
1
-
-
-
-
1
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
743380
Kozlova
Hypoxia-inducible factor prol ...
Homo sapiens
Oncotarget
8
9885-9898
2017
-
-
-
-
-
-
-
-
-
-
-
1
-
2
-
-
-
-
-
2
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
2
-
-
1
1
-
-
-
-
-
-
-
-
-
2
2
-
-
-
741826
Chen
Alternative splicing transcri ...
Megalobrama amblycephala
Biochem. Biophys. Res. Commun.
469
737-742
2016
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
10
-
-
1
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
10
-
-
1
1
-
-
-
-
-
-
-
-
1
1
1
1
-
-
743284
Kapitsinou
The endothelial prolyl-4-hydr ...
Mus musculus
Mol. Cell. Biol.
36
1584-1594
2016
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
-
1
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
743285
Arsenault
The zinc finger of prolyl hyd ...
Mus musculus
Mol. Cell. Biol.
36
2328-2343
2016
-
-
-
-
-
-
-
-
-
1
-
1
-
3
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
1
-
-
-
-
-
2
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
741910
Pektas
Increased turnover at limitin ...
Homo sapiens
Biochemistry
54
2851-2857
2015
-
-
1
-
2
-
-
9
-
-
-
1
-
2
-
-
1
-
-
-
-
-
2
1
-
-
-
9
-
-
-
-
-
-
-
-
-
1
-
-
2
-
-
-
-
9
-
-
-
1
-
-
-
1
-
-
-
-
2
1
-
-
-
9
-
-
-
-
-
-
-
-
8
8
742307
Bishop
HIF hydroxylase pathways in c ...
Homo sapiens
Circ. Res.
117
65-79
2015
-
-
-
-
-
-
2
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
742656
Wang
Current advances in the novel ...
Caenorhabditis elegans, Drosophila melanogaster
Insect Mol. Biol.
24
634-648
2015
-
-
-
-
-
-
-
-
-
-
-
2
-
7
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
2
2
-
-
-
743203
Giannetto
Hypoxia-inducible factor alph ...
Mytilus galloprovincialis
Mar. Biotechnol.
17
768-781
2015
-
-
-
-
-
-
-
-
-
-
-
1
-
7
-
-
-
-
-
1
-
-
1
-
-
-
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