Literature summary for 1.14.11.4 extracted from

  • Pornprasertsuk, S.; Duarte, W.R.; Mochida, Y.; Yamauchi, M.
    Lysyl hydroxylase-2b directs collagen cross-linking pathways in MC3T3-E1 cells (2004), J. Bone Miner. Res., 19, 1349-1355.
    View publication on PubMed

Cloned(Commentary)

Cloned (Comment) Organism
expression of active LH2b and of antisense construct in MC3T3-E1 cells, the latter suppresses the endogenous enzyme Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
procollagen L-lysine + 2-oxoglutarate + O2 Mus musculus isozyme LH2b directs the collagen cross-linking pathways, lysine hydroxylation as post-translational modification critical for collagen cross-linking and glycosylation, isozyme LH2 modulates the cross-linking pattern procollagen 5-hydroxy-L-lysine + succinate + CO2
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Organism

Organism UniProt Comment Textmining
Mus musculus
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3 isozymes LH1-3, isozyme LH2 occurs in 2 splicing variant, 2a and 2b, the first without insert
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Source Tissue

Source Tissue Comment Organism Textmining
kidney
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Mus musculus
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heart
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Mus musculus
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MC3T3-E1 cell different clones, osteoblastic cell line, expression of splicing vaiant 2b of isozyme LH2, but not of splicing variant 2a, expression analysis of the 3 isozymes in MC3T3-E1 cells and comparison of collagen cross-linking activities, overview Mus musculus
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Specific Activity [micromol/min/mg]

Specific Activity Minimum [µmol/min/mg] Specific Activity Maximum [µmol/min/mg] Comment Organism
additional information
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Mus musculus

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
procollagen L-lysine + 2-oxoglutarate + O2
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Mus musculus procollagen 5-hydroxy-L-lysine + succinate + CO2
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?
procollagen L-lysine + 2-oxoglutarate + O2 isozyme LH2b directs the collagen cross-linking pathways, lysine hydroxylation as post-translational modification critical for collagen cross-linking and glycosylation, isozyme LH2 modulates the cross-linking pattern Mus musculus procollagen 5-hydroxy-L-lysine + succinate + CO2
-
?

Synonyms

Synonyms Comment Organism
Lysyl hydroxylase-2b
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Mus musculus
LH2b
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Mus musculus