Literature summary for 1.14.11.4 extracted from

  • Sricholpech, M.; Perdivara, I.; Nagaoka, H.; Yokoyama, M.; Tomer, K.B.; Yamauchi, M.
    Lysyl hydroxylase 3 glucosylates galactosylhydroxylysine residues in type I collagen in osteoblast culture (2011), J. Biol. Chem., 286, 8846-8856.
    View publication on PubMedView publication on EuropePMC

Cloned(Commentary)

Cloned (Comment) Organism
gene Plod3, expression of recombinant V5/His-tagged LH3 protein in HEK-293 cells Mus musculus

Engineering

Protein Variants Comment Organism
additional information generation of mouse osteoblastic cell line, MC3T3-E1, stably suppressing Plod3 expresion using short hairpin RNA technology, reduced LH3 protein levels in the Sh clones, phenotype, overview Mus musculus

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
additional information Mus musculus lysyl hydroxylase 3 is the multifunctional collagen-modifying enzyme possessing lysyl hydroxylase, hydroxylysine galactosyltransferase, and galactosylhydroxylysine-glucosyltransferase activities, lysyl hydroxylase 3 glucosylates galactosylhydroxylysine residues in type I collagen ?
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?
additional information Mus musculus CRL-2593 lysyl hydroxylase 3 is the multifunctional collagen-modifying enzyme possessing lysyl hydroxylase, hydroxylysine galactosyltransferase, and galactosylhydroxylysine-glucosyltransferase activities, lysyl hydroxylase 3 glucosylates galactosylhydroxylysine residues in type I collagen ?
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?

Organism

Organism UniProt Comment Textmining
Mus musculus Q9R0E1 gene Plod3
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Mus musculus CRL-2593 Q9R0E1 gene Plod3
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Purification (Commentary)

Purification (Comment) Organism
recombinant V5/His-tagged LH3 protein from HEK-293 cells by nickel affinity chromatography, dialysis, and ultrafiltration Mus musculus

Source Tissue

Source Tissue Comment Organism Textmining
osteoblast
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Mus musculus
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MC3T3-E1 cell
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Mus musculus
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Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information lysyl hydroxylase 3 is the multifunctional collagen-modifying enzyme possessing lysyl hydroxylase, hydroxylysine galactosyltransferase, and galactosylhydroxylysine-glucosyltransferase activities, lysyl hydroxylase 3 glucosylates galactosylhydroxylysine residues in type I collagen Mus musculus ?
-
?
additional information lysyl hydroxylase 3 is the multifunctional collagen-modifying enzyme possessing lysyl hydroxylase, hydroxylysine galactosyltransferase, and galactosylhydroxylysine-glucosyltransferase activities, lysyl hydroxylase 3 glucosylates galactosylhydroxylysine residues in type I collagen Mus musculus CRL-2593 ?
-
?

Synonyms

Synonyms Comment Organism
LH
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Mus musculus
lysyl hydroxylase 3
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Mus musculus

General Information

General Information Comment Organism
physiological function lysyl hydroxylase 3 glucosylates galactosylhydroxylysine residues in type I collagen in osteoblast culture, role of LH3 in bone physiology Mus musculus
malfunction impairment of LH3 function significantly affects type I collagen fibrillogenesis Mus musculus