BRENDA - Enzyme Database
show all sequences of 1.14.11.48

Characterization of active site variants of xanthine hydroxylase from Aspergillus nidulans

Li, M.; Muller, T.A.; Fraser, B.A.; Hausinger, R.P.; Arch. Biochem. Biophys. 470, 44-53 (2008)

Data extracted from this reference:

Cloned(Commentary)
Cloned (Commentary)
Organism
wild-type and mutant enzymes
Aspergillus nidulans
Engineering
Protein Variants
Commentary
Organism
C357A
enhanced activity towards 9-methylxanthine as compared to wild-type enzyme. Mutant enzyme is resistant rto thiol-specific reagents that inactivated wild-type enzyme. Elevated ferroxidase activity in the absence of substrates
Aspergillus nidulans
D138A
enhanced activity towards 9-methylxanthine as compared to wild-type enzyme. Significant decrease in Ki(app) for 6,8-dihydroxypurine
Aspergillus nidulans
D151A
inactive mutant enzyme
Aspergillus nidulans
E137A
enhanced activity towards 9-methylxanthine as compared to wild-type enzyme. Significant decrease in Ki(app) for 6,8-dihydroxypurine
Aspergillus nidulans
H149A
inactive mutant enzyme
Aspergillus nidulans
H340A
mutant enzyme exhibits 0.17% of the wild-type enzyme activity
Aspergillus nidulans
K122A
2fold increase in the Kd of 2-oxoglutarate compared to wild-type enzyme
Aspergillus nidulans
N358A
23fold decrease in kcat/Km compared to wild-type enzyme. Significant decrease in Ki(app) for 6,8-dihydroxypurine
Aspergillus nidulans
Q101A
elevated ferroxidase activity in the absence of substrates
Aspergillus nidulans
Q356A
significant decrease in Ki(app) for 6,8-dihydroxypurine. Elevated ferroxidase activity in the absence of substrates
Aspergillus nidulans
Inhibitors
Inhibitors
Commentary
Organism
Structure
6,8-dihydroxypurine
slow-binding competitive inhibitor
Aspergillus nidulans
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.031
-
2-oxoglutarate
pH 7.4, 25°C, wild-type enzyme
Aspergillus nidulans
0.032
-
2-oxoglutarate
pH 7.4, 25°C, mutant enzyme K122A
Aspergillus nidulans
0.041
-
2-oxoglutarate
pH 7.4, 25°C, mutant enzyme E137A
Aspergillus nidulans
0.042
-
2-oxoglutarate
pH 7.4, 25°C, mutant enzyme Q356A
Aspergillus nidulans
0.045
-
xanthine
pH 7.4, 25°C, wild-type enzyme
Aspergillus nidulans
0.047
-
2-oxoglutarate
pH 7.4, 25°C, mutant enzyme C357A
Aspergillus nidulans
0.048
-
2-oxoglutarate
pH 7.4, 25°C, mutant enzyme N358A
Aspergillus nidulans
0.053
-
2-oxoglutarate
pH 7.4, 25°C, mutant enzyme D138A
Aspergillus nidulans
0.057
-
xanthine
pH 7.4, 25°C, mutant enzyme Q356A
Aspergillus nidulans
0.058
-
xanthine
pH 7.4, 25°C, mutant enzyme C357A
Aspergillus nidulans
0.059
-
2-oxoglutarate
pH 7.4, 25°C, mutant enzyme Q101A
Aspergillus nidulans
0.069
-
xanthine
pH 7.4, 25°C, mutant enzyme D138A
Aspergillus nidulans
0.071
-
xanthine
pH 7.4, 25°C, mutant enzyme E137A
Aspergillus nidulans
0.088
-
xanthine
pH 7.4, 25°C, mutant enzyme K122A
Aspergillus nidulans
0.122
-
xanthine
pH 7.4, 25°C, mutant enzyme Q101A
Aspergillus nidulans
0.4
-
9-methylxanthine
pH 7.4, 25°C, wild-type enzyme
Aspergillus nidulans
0.55
-
9-methylxanthine
pH 7.4, 25°C, mutant enzyme C357A
Aspergillus nidulans
0.554
-
xanthine
pH 7.4, 25°C, mutant enzyme N358A
Aspergillus nidulans
0.58
-
9-methylxanthine
pH 7.4, 25°C, mutant enzyme D138A
Aspergillus nidulans
0.68
-
9-methylxanthine
pH 7.4, 25°C, mutant enzyme Q356A
Aspergillus nidulans
0.78
-
9-methylxanthine
pH 7.4, 25°C, mutant enzyme Q101A
Aspergillus nidulans
0.88
-
9-methylxanthine
pH 7.4, 25°C, mutant enzyme K122A
Aspergillus nidulans
1
-
9-methylxanthine
pH 7.4, 25°C, mutant enzyme N358A
Aspergillus nidulans
1.09
-
9-methylxanthine
pH 7.4, 25°C, mutant enzyme E137A
Aspergillus nidulans
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Fe2+
non-heme mononuclear Fe(II) enzyme
Aspergillus nidulans
Organism
Organism
UniProt
Commentary
Textmining
Aspergillus nidulans
Q4QZZ9
-
-
Purification (Commentary)
Purification (Commentary)
Organism
wild-type enzyme variants are purified from recombinant Escherichia coli cells
Aspergillus nidulans
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
9-methylxanthine + 2-oxoglutarate + O2
-
731217
Aspergillus nidulans
?
-
-
-
?
xanthine + 2-oxoglutarate + O2
-
731217
Aspergillus nidulans
urate + succinate + CO2
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Aspergillus nidulans
Turnover Number [1/s]
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.66
-
9-methylxanthine
pH 7.4, 25°C, mutant enzyme Q101A
Aspergillus nidulans
1.26
-
9-methylxanthine
pH 7.4, 25°C, mutant enzyme N358A
Aspergillus nidulans
1.4
-
9-methylxanthine
pH 7.4, 25°C, mutant enzyme Q356A
Aspergillus nidulans
1.9
-
9-methylxanthine
pH 7.4, 25°C, mutant enzyme K122A
Aspergillus nidulans
2
8
2-oxoglutarate
pH 7.4, 25°C, mutant enzyme Q356A
Aspergillus nidulans
3
6
xanthine
pH 7.4, 25°C, mutant enzyme Q101A
Aspergillus nidulans
3.8
-
9-methylxanthine
pH 7.4, 25°C, wild-type enzyme
Aspergillus nidulans
4.25
-
9-methylxanthine
pH 7.4, 25°C, mutant enzyme D138A
Aspergillus nidulans
4.5
-
9-methylxanthine
pH 7.4, 25°C, mutant enzyme C357A
Aspergillus nidulans
5.9
-
9-methylxanthine
pH 7.4, 25°C, mutant enzyme E137A
Aspergillus nidulans
17
-
2-oxoglutarate
pH 7.4, 25°C, mutant enzyme N358A
Aspergillus nidulans
19
-
2-oxoglutarate
pH 7.4, 25°C, mutant enzyme C357A
Aspergillus nidulans
21
-
2-oxoglutarate
pH 7.4, 25°C, mutant enzyme Q101A
Aspergillus nidulans
25
-
2-oxoglutarate
pH 7.4, 25°C, mutant enzyme D138A
Aspergillus nidulans
31
-
2-oxoglutarate
pH 7.4, 25°C, mutant enzyme E137A
Aspergillus nidulans
32
-
xanthine
pH 7.4, 25°C, mutant enzyme C357A
Aspergillus nidulans
34
-
xanthine
pH 7.4, 25°C, mutant enzyme D138A
Aspergillus nidulans
35
-
xanthine
pH 7.4, 25°C, mutant enzyme Q356A
Aspergillus nidulans
38
-
xanthine
pH 7.4, 25°C, mutant enzyme N358A
Aspergillus nidulans
40
-
xanthine
pH 7.4, 25°C, mutant enzyme E137A
Aspergillus nidulans
42
-
2-oxoglutarate
pH 7.4, 25°C, mutant enzyme K122A
Aspergillus nidulans
60
-
xanthine
pH 7.4, 25°C, mutant enzyme K122A
Aspergillus nidulans
67
-
2-oxoglutarate
pH 7.4, 25°C, wild-type enzyme
Aspergillus nidulans
72
-
xanthine
pH 7.4, 25°C, wild-type enzyme
Aspergillus nidulans
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at
Aspergillus nidulans
Cloned(Commentary) (protein specific)
Commentary
Organism
wild-type and mutant enzymes
Aspergillus nidulans
Engineering (protein specific)
Protein Variants
Commentary
Organism
C357A
enhanced activity towards 9-methylxanthine as compared to wild-type enzyme. Mutant enzyme is resistant rto thiol-specific reagents that inactivated wild-type enzyme. Elevated ferroxidase activity in the absence of substrates
Aspergillus nidulans
D138A
enhanced activity towards 9-methylxanthine as compared to wild-type enzyme. Significant decrease in Ki(app) for 6,8-dihydroxypurine
Aspergillus nidulans
D151A
inactive mutant enzyme
Aspergillus nidulans
E137A
enhanced activity towards 9-methylxanthine as compared to wild-type enzyme. Significant decrease in Ki(app) for 6,8-dihydroxypurine
Aspergillus nidulans
H149A
inactive mutant enzyme
Aspergillus nidulans
H340A
mutant enzyme exhibits 0.17% of the wild-type enzyme activity
Aspergillus nidulans
K122A
2fold increase in the Kd of 2-oxoglutarate compared to wild-type enzyme
Aspergillus nidulans
N358A
23fold decrease in kcat/Km compared to wild-type enzyme. Significant decrease in Ki(app) for 6,8-dihydroxypurine
Aspergillus nidulans
Q101A
elevated ferroxidase activity in the absence of substrates
Aspergillus nidulans
Q356A
significant decrease in Ki(app) for 6,8-dihydroxypurine. Elevated ferroxidase activity in the absence of substrates
Aspergillus nidulans
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
6,8-dihydroxypurine
slow-binding competitive inhibitor
Aspergillus nidulans
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.031
-
2-oxoglutarate
pH 7.4, 25°C, wild-type enzyme
Aspergillus nidulans
0.032
-
2-oxoglutarate
pH 7.4, 25°C, mutant enzyme K122A
Aspergillus nidulans
0.041
-
2-oxoglutarate
pH 7.4, 25°C, mutant enzyme E137A
Aspergillus nidulans
0.042
-
2-oxoglutarate
pH 7.4, 25°C, mutant enzyme Q356A
Aspergillus nidulans
0.045
-
xanthine
pH 7.4, 25°C, wild-type enzyme
Aspergillus nidulans
0.047
-
2-oxoglutarate
pH 7.4, 25°C, mutant enzyme C357A
Aspergillus nidulans
0.048
-
2-oxoglutarate
pH 7.4, 25°C, mutant enzyme N358A
Aspergillus nidulans
0.053
-
2-oxoglutarate
pH 7.4, 25°C, mutant enzyme D138A
Aspergillus nidulans
0.057
-
xanthine
pH 7.4, 25°C, mutant enzyme Q356A
Aspergillus nidulans
0.058
-
xanthine
pH 7.4, 25°C, mutant enzyme C357A
Aspergillus nidulans
0.059
-
2-oxoglutarate
pH 7.4, 25°C, mutant enzyme Q101A
Aspergillus nidulans
0.069
-
xanthine
pH 7.4, 25°C, mutant enzyme D138A
Aspergillus nidulans
0.071
-
xanthine
pH 7.4, 25°C, mutant enzyme E137A
Aspergillus nidulans
0.088
-
xanthine
pH 7.4, 25°C, mutant enzyme K122A
Aspergillus nidulans
0.122
-
xanthine
pH 7.4, 25°C, mutant enzyme Q101A
Aspergillus nidulans
0.4
-
9-methylxanthine
pH 7.4, 25°C, wild-type enzyme
Aspergillus nidulans
0.55
-
9-methylxanthine
pH 7.4, 25°C, mutant enzyme C357A
Aspergillus nidulans
0.554
-
xanthine
pH 7.4, 25°C, mutant enzyme N358A
Aspergillus nidulans
0.58
-
9-methylxanthine
pH 7.4, 25°C, mutant enzyme D138A
Aspergillus nidulans
0.68
-
9-methylxanthine
pH 7.4, 25°C, mutant enzyme Q356A
Aspergillus nidulans
0.78
-
9-methylxanthine
pH 7.4, 25°C, mutant enzyme Q101A
Aspergillus nidulans
0.88
-
9-methylxanthine
pH 7.4, 25°C, mutant enzyme K122A
Aspergillus nidulans
1
-
9-methylxanthine
pH 7.4, 25°C, mutant enzyme N358A
Aspergillus nidulans
1.09
-
9-methylxanthine
pH 7.4, 25°C, mutant enzyme E137A
Aspergillus nidulans
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Fe2+
non-heme mononuclear Fe(II) enzyme
Aspergillus nidulans
Purification (Commentary) (protein specific)
Commentary
Organism
wild-type enzyme variants are purified from recombinant Escherichia coli cells
Aspergillus nidulans
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
9-methylxanthine + 2-oxoglutarate + O2
-
731217
Aspergillus nidulans
?
-
-
-
?
xanthine + 2-oxoglutarate + O2
-
731217
Aspergillus nidulans
urate + succinate + CO2
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
25
-
assay at
Aspergillus nidulans
Turnover Number [1/s] (protein specific)
Turnover Number Minimum [1/s]
Turnover Number Maximum [1/s]
Substrate
Commentary
Organism
Structure
0.66
-
9-methylxanthine
pH 7.4, 25°C, mutant enzyme Q101A
Aspergillus nidulans
1.26
-
9-methylxanthine
pH 7.4, 25°C, mutant enzyme N358A
Aspergillus nidulans
1.4
-
9-methylxanthine
pH 7.4, 25°C, mutant enzyme Q356A
Aspergillus nidulans
1.9
-
9-methylxanthine
pH 7.4, 25°C, mutant enzyme K122A
Aspergillus nidulans
2
8
2-oxoglutarate
pH 7.4, 25°C, mutant enzyme Q356A
Aspergillus nidulans
3
6
xanthine
pH 7.4, 25°C, mutant enzyme Q101A
Aspergillus nidulans
3.8
-
9-methylxanthine
pH 7.4, 25°C, wild-type enzyme
Aspergillus nidulans
4.25
-
9-methylxanthine
pH 7.4, 25°C, mutant enzyme D138A
Aspergillus nidulans
4.5
-
9-methylxanthine
pH 7.4, 25°C, mutant enzyme C357A
Aspergillus nidulans
5.9
-
9-methylxanthine
pH 7.4, 25°C, mutant enzyme E137A
Aspergillus nidulans
17
-
2-oxoglutarate
pH 7.4, 25°C, mutant enzyme N358A
Aspergillus nidulans
19
-
2-oxoglutarate
pH 7.4, 25°C, mutant enzyme C357A
Aspergillus nidulans
21
-
2-oxoglutarate
pH 7.4, 25°C, mutant enzyme Q101A
Aspergillus nidulans
25
-
2-oxoglutarate
pH 7.4, 25°C, mutant enzyme D138A
Aspergillus nidulans
31
-
2-oxoglutarate
pH 7.4, 25°C, mutant enzyme E137A
Aspergillus nidulans
32
-
xanthine
pH 7.4, 25°C, mutant enzyme C357A
Aspergillus nidulans
34
-
xanthine
pH 7.4, 25°C, mutant enzyme D138A
Aspergillus nidulans
35
-
xanthine
pH 7.4, 25°C, mutant enzyme Q356A
Aspergillus nidulans
38
-
xanthine
pH 7.4, 25°C, mutant enzyme N358A
Aspergillus nidulans
40
-
xanthine
pH 7.4, 25°C, mutant enzyme E137A
Aspergillus nidulans
42
-
2-oxoglutarate
pH 7.4, 25°C, mutant enzyme K122A
Aspergillus nidulans
60
-
xanthine
pH 7.4, 25°C, mutant enzyme K122A
Aspergillus nidulans
67
-
2-oxoglutarate
pH 7.4, 25°C, wild-type enzyme
Aspergillus nidulans
72
-
xanthine
pH 7.4, 25°C, wild-type enzyme
Aspergillus nidulans
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7.4
-
assay at
Aspergillus nidulans
KCat/KM [mM/s]
kcat/KM Value [1/mMs-1]
kcat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
70
-
xanthine
pH 7.4, 25°C, mutant enzyme N358A
Aspergillus nidulans
300
-
xanthine
pH 7.4, 25°C, mutant enzyme Q101A
Aspergillus nidulans
340
-
2-oxoglutarate
pH 7.4, 25°C, mutant enzyme N358A
Aspergillus nidulans
360
-
2-oxoglutarate
pH 7.4, 25°C, mutant enzyme Q101A
Aspergillus nidulans
400
-
2-oxoglutarate
pH 7.4, 25°C, mutant enzyme C357A
Aspergillus nidulans
470
-
2-oxoglutarate
pH 7.4, 25°C, mutant enzyme D138A
Aspergillus nidulans
490
-
xanthine
pH 7.4, 25°C, mutant enzyme D138A
Aspergillus nidulans
550
-
xanthine
pH 7.4, 25°C, mutant enzyme C357A
Aspergillus nidulans
560
-
xanthine
pH 7.4, 25°C, mutant enzyme E137A
Aspergillus nidulans
610
-
xanthine
pH 7.4, 25°C, mutant enzyme Q356A
Aspergillus nidulans
670
-
2-oxoglutarate
pH 7.4, 25°C, mutant enzyme Q356A
Aspergillus nidulans
680
-
xanthine
pH 7.4, 25°C, mutant enzyme K122A
Aspergillus nidulans
760
-
2-oxoglutarate
pH 7.4, 25°C, mutant enzyme E137A
Aspergillus nidulans
850
-
9-methylxanthine
pH 7.4, 25°C, mutant enzyme Q101A
Aspergillus nidulans
1260
-
9-methylxanthine
pH 7.4, 25°C, mutant enzyme N358A
Aspergillus nidulans
1310
-
2-oxoglutarate
pH 7.4, 25°C, mutant enzyme K122A
Aspergillus nidulans
1600
-
xanthine
pH 7.4, 25°C, wild-type enzyme
Aspergillus nidulans
2050
-
9-methylxanthine
pH 7.4, 25°C, mutant enzyme Q356A
Aspergillus nidulans
2160
-
9-methylxanthine
pH 7.4, 25°C, mutant enzyme K122A
Aspergillus nidulans
2160
-
2-oxoglutarate
pH 7.4, 25°C, wild-type enzyme
Aspergillus nidulans
5410
-
9-methylxanthine
pH 7.4, 25°C, mutant enzyme E137A
Aspergillus nidulans
7330
-
9-methylxanthine
pH 7.4, 25°C, mutant enzyme D138A
Aspergillus nidulans
8180
-
9-methylxanthine
pH 7.4, 25°C, mutant enzyme C357A
Aspergillus nidulans
9500
-
9-methylxanthine
pH 7.4, 25°C, wild-type enzyme
Aspergillus nidulans
KCat/KM [mM/s] (protein specific)
KCat/KM Value [1/mMs-1]
KCat/KM Value Maximum [1/mMs-1]
Substrate
Commentary
Organism
Structure
70
-
xanthine
pH 7.4, 25°C, mutant enzyme N358A
Aspergillus nidulans
300
-
xanthine
pH 7.4, 25°C, mutant enzyme Q101A
Aspergillus nidulans
340
-
2-oxoglutarate
pH 7.4, 25°C, mutant enzyme N358A
Aspergillus nidulans
360
-
2-oxoglutarate
pH 7.4, 25°C, mutant enzyme Q101A
Aspergillus nidulans
400
-
2-oxoglutarate
pH 7.4, 25°C, mutant enzyme C357A
Aspergillus nidulans
470
-
2-oxoglutarate
pH 7.4, 25°C, mutant enzyme D138A
Aspergillus nidulans
490
-
xanthine
pH 7.4, 25°C, mutant enzyme D138A
Aspergillus nidulans
550
-
xanthine
pH 7.4, 25°C, mutant enzyme C357A
Aspergillus nidulans
560
-
xanthine
pH 7.4, 25°C, mutant enzyme E137A
Aspergillus nidulans
610
-
xanthine
pH 7.4, 25°C, mutant enzyme Q356A
Aspergillus nidulans
670
-
2-oxoglutarate
pH 7.4, 25°C, mutant enzyme Q356A
Aspergillus nidulans
680
-
xanthine
pH 7.4, 25°C, mutant enzyme K122A
Aspergillus nidulans
760
-
2-oxoglutarate
pH 7.4, 25°C, mutant enzyme E137A
Aspergillus nidulans
850
-
9-methylxanthine
pH 7.4, 25°C, mutant enzyme Q101A
Aspergillus nidulans
1260
-
9-methylxanthine
pH 7.4, 25°C, mutant enzyme N358A
Aspergillus nidulans
1310
-
2-oxoglutarate
pH 7.4, 25°C, mutant enzyme K122A
Aspergillus nidulans
1600
-
xanthine
pH 7.4, 25°C, wild-type enzyme
Aspergillus nidulans
2050
-
9-methylxanthine
pH 7.4, 25°C, mutant enzyme Q356A
Aspergillus nidulans
2160
-
9-methylxanthine
pH 7.4, 25°C, mutant enzyme K122A
Aspergillus nidulans
2160
-
2-oxoglutarate
pH 7.4, 25°C, wild-type enzyme
Aspergillus nidulans
5410
-
9-methylxanthine
pH 7.4, 25°C, mutant enzyme E137A
Aspergillus nidulans
7330
-
9-methylxanthine
pH 7.4, 25°C, mutant enzyme D138A
Aspergillus nidulans
8180
-
9-methylxanthine
pH 7.4, 25°C, mutant enzyme C357A
Aspergillus nidulans
9500
-
9-methylxanthine
pH 7.4, 25°C, wild-type enzyme
Aspergillus nidulans
Other publictions for EC 1.14.11.48
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
731217
Li
Characterization of active sit ...
Aspergillus nidulans
Arch. Biochem. Biophys.
470
44-53
2008
-
-
1
-
10
-
1
24
-
1
-
-
-
1
-
-
1
-
-
-
-
-
2
-
-
1
-
-
24
1
-
-
-
-
-
-
-
-
1
-
-
10
-
-
1
-
24
-
1
-
-
-
-
-
1
-
-
-
-
2
-
1
-
-
24
1
-
-
-
-
-
-
-
24
24
731289
Montero-Moran
Purification and characterizat ...
Aspergillus nidulans
Biochemistry
46
5293-5304
2007
-
-
1
-
-
-
8
5
-
1
2
-
-
4
-
1
1
-
-
1
-
3
3
1
2
-
-
-
5
2
-
1
-
1
-
-
-
-
1
-
-
-
-
-
8
1
5
-
1
2
-
-
-
1
1
-
1
-
3
3
1
-
-
-
5
2
-
1
-
-
-
-
-
-
-
732527
Cultrone
Convergent evolution of hydrox ...
Aspergillus nidulans
Mol. Microbiol.
57
276-290
2005
-
-
1
-
-
-
1
2
-
1
-
1
-
1
-
-
-
-
-
-
-
-
1
-
2
1
-
-
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
1
-
2
-
1
-
1
-
-
-
-
-
-
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-