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Literature summary for 1.14.11.67 extracted from

  • Spedaletti, V.; Polticelli, F.; Capodaglio, V.; Schinina, M.E.; Stano, P.; Federico, R.; Tavladoraki, P.
    Characterization of a lysine-specific histone demethylase from Arabidopsis thaliana (2008), Biochemistry, 47, 4936-4947 .
    View publication on PubMed
Search for more literature for 1.14.11.67

Activating Compound

Activating Compound Comment Organism Structure
glycerol 50% increase of activity in the presence of 30% (v/v) glycerol as compared to that in the presence of 0-10% (v/v) glycerol Arabidopsis thaliana

Cloned(Commentary)

Cloned (Comment) Organism
expressed in Escherichia coli BL21(DE3) cells Arabidopsis thaliana

Inhibitors

Inhibitors Comment Organism Structure
K+ strong inhibition at 50 mM Arabidopsis thaliana
Mg2+ 75% inhibition at 50 mM Arabidopsis thaliana

KM Value [mM]

KM Value [mM] KM Value Maximum [mM] Substrate Comment Organism Structure
0.0089
-
 [histone H3]-N6-methyl-L-lysine4 at pH 8.0 and 25°C Arabidopsis thaliana
0.0107
-
 [histone H3]-N6,N6-dimethyl-L-lysine4 at pH 8.0 and 25°C Arabidopsis thaliana
0.0128
-
 [histone H3]-N6,N6-dimethyl-L-lysine4 in 10% (v/v) glycerol, at pH 8.0 and 25°C Arabidopsis thaliana

Natural Substrates/ Products (Substrates)

Natural Substrates Organism Comment (Nat. Sub.) Natural Products Comment (Nat. Pro.) Rev. Reac.
[histone H3]-N6,N6-dimethyl-L-lysine4 + 2-oxoglutarate + O2 Arabidopsis thaliana
-
 [histone H3]-N6-methyl-L-lysine4 + succinate + formaldehyde + CO2
-
 ?
[histone H3]-N6-methyl-L-lysine4 + 2-oxoglutarate + O2 Arabidopsis thaliana
-
 [histone H3]-L-lysine4 + succinate + formaldehyde + CO2
-
 ?

Organism

Organism UniProt Comment Textmining
Arabidopsis thaliana
-
-
-

Purification (Commentary)

Purification (Comment) Organism
Ni2+-charged resin column chromatography and HiTrap heparin column chromatography Arabidopsis thaliana

Substrates and Products (Substrate)

Substrates Comment Substrates Organism Products Comment (Products) Rev. Reac.
additional information no activity with [histone H3]-N6,N6-dimethyl-L-lysine9, [histone H3]-N6,N6-dimethyl-L-lysine27, spermine, spermidine, and putrescine Arabidopsis thaliana ?
-
-
[histone H3]-N6,N6-dimethyl-L-lysine4 + 2-oxoglutarate + O2
-
 Arabidopsis thaliana [histone H3]-N6-methyl-L-lysine4 + succinate + formaldehyde + CO2
-
 ?
[histone H3]-N6-methyl-L-lysine4 + 2-oxoglutarate + O2
-
 Arabidopsis thaliana [histone H3]-L-lysine4 + succinate + formaldehyde + CO2
-
 ?

Subunits

Subunits Comment Organism
? x * 94000, SDS-PAGE Arabidopsis thaliana

Synonyms

Synonyms Comment Organism
histone H3 lysine 4 demethylase
-
 Arabidopsis thaliana
LSD1
-
 Arabidopsis thaliana
lysine-specific demethylase 1
-
 Arabidopsis thaliana

Turnover Number [1/s]

Turnover Number Minimum [1/s] Turnover Number Maximum [1/s] Substrate Comment Organism Structure
0.0057
-
 [histone H3]-N6,N6-dimethyl-L-lysine4 in 10% (v/v) glycerol, at pH 8.0 and 25°C Arabidopsis thaliana
0.0083
-
 [histone H3]-N6-methyl-L-lysine4 at pH 8.0 and 25°C Arabidopsis thaliana
0.0113
-
 [histone H3]-N6,N6-dimethyl-L-lysine4 at pH 8.0 and 25°C Arabidopsis thaliana

pH Optimum

pH Optimum Minimum pH Optimum Maximum Comment Organism
8
-
-
 Arabidopsis thaliana

IC50 Value

IC50 Value IC50 Value Maximum Comment Organism Inhibitor Structure
50
-
 at pH 8.0 and 25°C Arabidopsis thaliana K+