Cloned (Comment) | Organism |
---|---|
gene F3H, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, semi-quantitative RT-PCR enzyme expression analysis | Nelumbo nucifera |
gene F3H, DNA and amino acid sequence determination and analysis, sequence comparisons and phylogenetic analysis, semi-quantitative RT-PCR enzyme expression analysis | Nymphaea hybrid cultivar |
Protein Variants | Comment | Organism |
---|---|---|
additional information | method evaluation for RNAi-mediated silencing downregulation of F3H expression, introduction of RNAi gene-silencing vector, pJA8F3H, encoding a hairpin F3H RNA, to waterlily petals using the Agrobacterium tumefaciens strain GV3101 infiltration method, F3H expression is analysed at 1 and 3 days post infiltration semi-quantitative RT-PCR, showing that F3H expression is downregulated at 3 dpi in flowers tested of the red petal variety and purplish blue petal variety compared to controls. Vector pJA8F3H is efficient and can be used as a transformation vector to transiently suppress F3H expression in waterlily or lotus | Nymphaea hybrid cultivar |
additional information | method evaluation for RNAi-mediated silencing downregulation of F3H expression, vector pJA8F3H is efficient and can be used as a transformation vector to transiently suppress F3H expression in waterlily or lotus | Nelumbo nucifera |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
a (2S)-flavan-4-one + 2-oxoglutarate + O2 | Nelumbo nucifera | - |
a (2R,3R)-dihydroflavonol + succinate + CO2 | - |
? | |
a (2S)-flavan-4-one + 2-oxoglutarate + O2 | Nymphaea hybrid cultivar | - |
a (2R,3R)-dihydroflavonol + succinate + CO2 | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Nelumbo nucifera | V9P4G8 | cv. Buntharik (white petal lotus) and cv. Satabankacha (pink petal lotus) | - |
Nymphaea hybrid cultivar | V9P4H2 | var. St. Louis Gold | - |
Source Tissue | Comment | Organism | Textmining |
---|---|---|---|
leaf | low expression level | Nymphaea hybrid cultivar | - |
additional information | expression of F3H is specifically regulated in petals and stamens. The F3H expression is slightly diminished in petals of cultivar Satabankacha at the fully-opening stage, whereas it is detected in the cultivar Buntharik only when white petals are tinted with pink. | Nelumbo nucifera | - |
petal | - |
Nelumbo nucifera | - |
petal | - |
Nymphaea hybrid cultivar | - |
stamen | - |
Nelumbo nucifera | - |
stamen | - |
Nymphaea hybrid cultivar | - |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
a (2S)-flavan-4-one + 2-oxoglutarate + O2 | - |
Nelumbo nucifera | a (2R,3R)-dihydroflavonol + succinate + CO2 | - |
? | |
a (2S)-flavan-4-one + 2-oxoglutarate + O2 | - |
Nymphaea hybrid cultivar | a (2R,3R)-dihydroflavonol + succinate + CO2 | - |
? |
Synonyms | Comment | Organism |
---|---|---|
F3H | - |
Nelumbo nucifera |
F3H | - |
Nymphaea hybrid cultivar |
flavanone 3-hydroxylase | - |
Nelumbo nucifera |
flavanone 3-hydroxylase | - |
Nymphaea hybrid cultivar |
General Information | Comment | Organism |
---|---|---|
physiological function | F3H is a key regulator for the flavonoid biosynthesis pathway and plays a role in accumulation of anthocyanin pigments in the vacuole | Nelumbo nucifera |
physiological function | F3H is a key regulator for the flavonoid biosynthesis pathway and plays a role in accumulation of anthocyanin pigments in the vacuole | Nymphaea hybrid cultivar |