Activating Compound | Comment | Organism | Structure |
---|---|---|---|
2-mercaptoethanol | required for maximal activity, 292.3% activity at 1% (v/v) | Burkholderia sp. | |
ATP | 124% activity at 0.1 mM | Burkholderia sp. | |
FMN | 230.7% activity at 0.1 mM | Burkholderia sp. | |
GTP | 116% activity at 0.1 mM | Burkholderia sp. | |
lipase | 176% activity at 1 mg per g wet weight of cells | Burkholderia sp. | |
NADP+ | 2fold enhancement of activity in the presence of NADP+ | Burkholderia sp. | |
phosphatidylcholine | 125% activity at 0.05 mg | Burkholderia sp. | |
phosphatidylethanolamine | 144% activity at 0.1 mg per g wet weight of cells | Burkholderia sp. | |
Phospholipase A | 125% activity at 0.01 mg | Burkholderia sp. |
Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli PFJS39 cells | Burkholderia sp. |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
deoxycholate | about 68% residual activity at 6% (v/v) | Burkholderia sp. | |
Dodecyl sulfate | about 40% residual activity at 1% (v/v) | Burkholderia sp. | |
FAD | - |
Burkholderia sp. | |
spermidine | 80% residual activity at 0.05 mg | Burkholderia sp. | |
Triton X-100 | about 50% residual activity at 2% (v/v) | Burkholderia sp. | |
Tween 20 | about 36% residual activity at 2% (v/v) | Burkholderia sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.05 | - |
O2 | at pH 7.0 and 37°C | Burkholderia sp. | |
0.18 | - |
2,4-dinitrotoluene | at pH 7.0 and 37°C | Burkholderia sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Fe2+ | required for activity, 238.5% activity at 1 mM | Burkholderia sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
100000 | - |
gel filtration | Burkholderia sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4-dinitrotoluene + NADH + H+ + O2 | Burkholderia sp. | - |
4-methyl-5-nitrocatechol + nitrite + NAD+ | - |
? | |
2,4-dinitrotoluene + NADH + H+ + O2 | Burkholderia sp. DNT | - |
4-methyl-5-nitrocatechol + nitrite + NAD+ | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Burkholderia sp. | - |
- |
- |
Burkholderia sp. DNT | - |
- |
- |
Purification (Comment) | Organism |
---|---|
ammonium sulfate precipitation, column chromatography, and Sephadex G-100 gel filtration | Burkholderia sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.4 | - |
crude extract, at pH 7.0 and 37°C | Burkholderia sp. |
37.3 | - |
after 15.7fold, at pH 7.0 and 37°C | Burkholderia sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,4-dinitrotoluene + NADH + H+ + O2 | - |
Burkholderia sp. | 4-methyl-5-nitrocatechol + nitrite + NAD+ | - |
? | |
2,4-dinitrotoluene + NADH + H+ + O2 | - |
Burkholderia sp. DNT | 4-methyl-5-nitrocatechol + nitrite + NAD+ | - |
? |
Subunits | Comment | Organism |
---|---|---|
heterotrimer | 1 * 52000-71000, 1 * 23000-25500, 1 * 12000-16500, SDS-PAGE | Burkholderia sp. |
Synonyms | Comment | Organism |
---|---|---|
DNT dioxygenase | - |
Burkholderia sp. |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
- |
Burkholderia sp. |
Temperature Minimum [°C] | Temperature Maximum [°C] | Comment | Organism |
---|---|---|---|
4 | 45 | activity rapidly diminishes when the enzyme is heated above 45°C. At 20°C, the activity is decreased about 25% compared with 37°C. At 15°C, the activity decreases about 85% compared with 37°C, and at 4-5°C, the activity is totally absent | Burkholderia sp. |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
- |
4 | the enzyme shows 5% to 15% loss in catalytic activity when left at 0-4°C for several weeks | Burkholderia sp. |
25 | 37 | the enzyme is stable for 2 days when incubated with substrate at 37°C, 30°C, and 25°C | Burkholderia sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
7 | - |
- |
Burkholderia sp. |
pH Minimum | pH Maximum | Comment | Organism |
---|---|---|---|
4 | 10 | the enzyme is stable when incubated at pH 6 and 7, but its optimal activity is at pH 7.0. The activity is decreased significantly when incubated at pH 4.0, 5.0, 8.0, and 10.0 | Burkholderia sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Burkholderia sp. | |
NAD+ | 2fold enhancement of activity in the presence of NAD+ | Burkholderia sp. | |
NADH | 5fold enhancement of activity in the presence of NADH | Burkholderia sp. | |
NADP+ | 2fold enhancement of activity in the presence of NADP+ | Burkholderia sp. |