BRENDA - Enzyme Database
show all sequences of 1.14.12.9

Purification and some properties of component B of the 4-chlorophenylacetate 3,4-dioxygenase from Pseudomonas species strain CBS 3

Schweizer, D.; Markus, A.; Seez, M.; Ruf, H.H.; Lingens, F.; J. Biol. Chem. 262, 9340-9346 (1987) View publication on PubMed

Data extracted from this reference:

General Stability
General Stability
Organism
75% loss of activity after 16 h dialysis
Pseudomonas sp.
Inhibitors
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
1 mM, 34% inhibition
Pseudomonas sp.
EDTA
1 mM, 32% inhibition
Pseudomonas sp.
iodoacetate
0.01 mM, 77% inhibition
Pseudomonas sp.
KCN
1 mM, 52% inhibition
Pseudomonas sp.
NEM
1 mM, 38% inhibition
Pseudomonas sp.
NH4+
inhibition of activity of reductase component
Pseudomonas sp.
PCMB
0.005 mM, complete inhibition
Pseudomonas sp.
KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0023
-
ferrocytochrome c
-
Pseudomonas sp.
0.0063
-
reduced 2,6-dichlorophenolindophenol
-
Pseudomonas sp.
0.032
-
NADH
-
Pseudomonas sp.
Metals/Ions
Metals/Ions
Commentary
Organism
Structure
Iron
reductase component contains an iron-sulfur cluster of the [2Fe-2S]-type, contains 2.1 mol of iron and 1.7 mol of acid-labile sulfide per mol of reductase
Pseudomonas sp.
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35000
-
reductase component of 4-chlorophenylacetate 3,4-dioxygenase, gel filtration
Pseudomonas sp.
Organism
Organism
UniProt
Commentary
Textmining
Pseudomonas sp.
-
-
-
Pseudomonas sp. CBS 3
-
-
-
Oxidation Stability
Oxidation Stability
Organism
reductase component loses activity in presence of molecular oxygen
Pseudomonas sp.
Purification (Commentary)
Purification (Commentary)
Organism
reductase component
Pseudomonas sp.
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
30
-
reductase component of of 4-chlorophenylacetate 3,4-dioxygenase
Pseudomonas sp.
Storage Stability
Storage Stability
Organism
4°C, 20 mM potassium phosphate buffer, pH 6.0-7.5, 10 days, 50% loss of activity
Pseudomonas sp.
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
Substrate Product ID
4-chlorophenylacetate + ferrocytochrome c + H+ + O2
-
439043
Pseudomonas sp.
3,4-dihydroxyphenylacetate + chloride + ferricytochrome c
-
-
-
r
4-chlorophenylacetate + ferrocytochrome c + H+ + O2
-
439043
Pseudomonas sp. CBS 3
3,4-dihydroxyphenylacetate + chloride + ferricytochrome c
-
-
-
r
4-chlorophenylacetate + NADH + O2 + H2O
-
439043
Pseudomonas sp.
3,4-dihydroxyphenylacetate + Cl- + NAD(P)+
-
-
-
?
4-chlorophenylacetate + NADH + O2 + H2O
-
439043
Pseudomonas sp. CBS 3
3,4-dihydroxyphenylacetate + Cl- + NAD(P)+
-
-
-
?
4-chlorophenylacetate + reduced 2,6-dichlorophenolindophenol + O2
-
439043
Pseudomonas sp.
3,4-dihydroxyphenylacetate + chloride + oxidized 2,6-dichlorophenolindophenol
-
-
-
r
4-chlorophenylacetate + reduced 2,6-dichlorophenolindophenol + O2
-
439043
Pseudomonas sp. CBS 3
3,4-dihydroxyphenylacetate + chloride + oxidized 2,6-dichlorophenolindophenol
-
-
-
r
additional information
the reductase component reduces several redox compounds: 2,6-dichlorophenylindophenol, potassium hexacyanoferrate III, cytochrome c, methylene blue and nitro blue tetrazolium
439043
Pseudomonas sp.
?
-
-
-
?
additional information
the reductase component reduces several redox compounds: 2,6-dichlorophenylindophenol, potassium hexacyanoferrate III, cytochrome c, methylene blue and nitro blue tetrazolium
439043
Pseudomonas sp. CBS 3
?
-
-
-
?
Subunits
Subunits
Commentary
Organism
monomer
1 * 35000, reductase component of of 4-chlorophenylacetate 3,4-dioxygenase, SDS-PAGE
Pseudomonas sp.
More
the enzyme consists of two components, a monomeric reductase and a trimeric dioxygenase
Pseudomonas sp.
Temperature Stability [°C]
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
45
-
3 min, 50% loss of activity
Pseudomonas sp.
55
-
90-100% loss of activity
Pseudomonas sp.
pH Optimum
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
7.5
-
Pseudomonas sp.
pH Stability
pH Stability
pH Stability Maximum
Commentary
Organism
6
7.5
4°C, 10 days, 50% loss of activity
Pseudomonas sp.
Cofactor
Cofactor
Commentary
Organism
Structure
FMN
flavoprotein, contains 1 mol of FMN per mol of reductase, no increase of activity by addition of exogenous FMN
Pseudomonas sp.
NADH
-
Pseudomonas sp.
NADPH
30% as effective as NADH
Pseudomonas sp.
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FMN
flavoprotein, contains 1 mol of FMN per mol of reductase, no increase of activity by addition of exogenous FMN
Pseudomonas sp.
NADH
-
Pseudomonas sp.
NADPH
30% as effective as NADH
Pseudomonas sp.
General Stability (protein specific)
General Stability
Organism
75% loss of activity after 16 h dialysis
Pseudomonas sp.
Inhibitors (protein specific)
Inhibitors
Commentary
Organism
Structure
1,10-phenanthroline
1 mM, 34% inhibition
Pseudomonas sp.
EDTA
1 mM, 32% inhibition
Pseudomonas sp.
iodoacetate
0.01 mM, 77% inhibition
Pseudomonas sp.
KCN
1 mM, 52% inhibition
Pseudomonas sp.
NEM
1 mM, 38% inhibition
Pseudomonas sp.
NH4+
inhibition of activity of reductase component
Pseudomonas sp.
PCMB
0.005 mM, complete inhibition
Pseudomonas sp.
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.0023
-
ferrocytochrome c
-
Pseudomonas sp.
0.0063
-
reduced 2,6-dichlorophenolindophenol
-
Pseudomonas sp.
0.032
-
NADH
-
Pseudomonas sp.
Metals/Ions (protein specific)
Metals/Ions
Commentary
Organism
Structure
Iron
reductase component contains an iron-sulfur cluster of the [2Fe-2S]-type, contains 2.1 mol of iron and 1.7 mol of acid-labile sulfide per mol of reductase
Pseudomonas sp.
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
35000
-
reductase component of 4-chlorophenylacetate 3,4-dioxygenase, gel filtration
Pseudomonas sp.
Oxidation Stability (protein specific)
Oxidation Stability
Organism
reductase component loses activity in presence of molecular oxygen
Pseudomonas sp.
Purification (Commentary) (protein specific)
Commentary
Organism
reductase component
Pseudomonas sp.
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
30
-
reductase component of of 4-chlorophenylacetate 3,4-dioxygenase
Pseudomonas sp.
Storage Stability (protein specific)
Storage Stability
Organism
4°C, 20 mM potassium phosphate buffer, pH 6.0-7.5, 10 days, 50% loss of activity
Pseudomonas sp.
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
ID
4-chlorophenylacetate + ferrocytochrome c + H+ + O2
-
439043
Pseudomonas sp.
3,4-dihydroxyphenylacetate + chloride + ferricytochrome c
-
-
-
r
4-chlorophenylacetate + ferrocytochrome c + H+ + O2
-
439043
Pseudomonas sp. CBS 3
3,4-dihydroxyphenylacetate + chloride + ferricytochrome c
-
-
-
r
4-chlorophenylacetate + NADH + O2 + H2O
-
439043
Pseudomonas sp.
3,4-dihydroxyphenylacetate + Cl- + NAD(P)+
-
-
-
?
4-chlorophenylacetate + NADH + O2 + H2O
-
439043
Pseudomonas sp. CBS 3
3,4-dihydroxyphenylacetate + Cl- + NAD(P)+
-
-
-
?
4-chlorophenylacetate + reduced 2,6-dichlorophenolindophenol + O2
-
439043
Pseudomonas sp.
3,4-dihydroxyphenylacetate + chloride + oxidized 2,6-dichlorophenolindophenol
-
-
-
r
4-chlorophenylacetate + reduced 2,6-dichlorophenolindophenol + O2
-
439043
Pseudomonas sp. CBS 3
3,4-dihydroxyphenylacetate + chloride + oxidized 2,6-dichlorophenolindophenol
-
-
-
r
additional information
the reductase component reduces several redox compounds: 2,6-dichlorophenylindophenol, potassium hexacyanoferrate III, cytochrome c, methylene blue and nitro blue tetrazolium
439043
Pseudomonas sp.
?
-
-
-
?
additional information
the reductase component reduces several redox compounds: 2,6-dichlorophenylindophenol, potassium hexacyanoferrate III, cytochrome c, methylene blue and nitro blue tetrazolium
439043
Pseudomonas sp. CBS 3
?
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
monomer
1 * 35000, reductase component of of 4-chlorophenylacetate 3,4-dioxygenase, SDS-PAGE
Pseudomonas sp.
More
the enzyme consists of two components, a monomeric reductase and a trimeric dioxygenase
Pseudomonas sp.
Temperature Stability [°C] (protein specific)
Temperature Stability Minimum [°C]
Temperature Stability Maximum [°C]
Commentary
Organism
45
-
3 min, 50% loss of activity
Pseudomonas sp.
55
-
90-100% loss of activity
Pseudomonas sp.
pH Optimum (protein specific)
pH Optimum Minimum
pH Optimum Maximum
Commentary
Organism
7
7.5
-
Pseudomonas sp.
pH Stability (protein specific)
pH Stability
pH Stability Maximum
Commentary
Organism
6
7.5
4°C, 10 days, 50% loss of activity
Pseudomonas sp.
Other publictions for EC 1.14.12.9
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Synonyms
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
439043
Schweizer
Purification and some properti ...
Pseudomonas sp., Pseudomonas sp. CBS 3
J. Biol. Chem.
262
9340-9346
1987
-
-
-
-
-
1
7
3
-
1
1
-
-
7
1
-
1
-
-
-
1
1
8
2
-
-
-
2
-
1
-
1
3
-
-
-
-
-
-
3
-
-
1
-
7
-
3
-
1
1
-
-
1
-
1
-
-
1
1
8
2
-
-
2
-
1
-
1
-
-
-
-
-
-
-
439042
Markus
Purification and some properti ...
Pseudomonas sp., Pseudomonas sp. CBS 3
J. Biol. Chem.
261
12883-12888
1986
-
-
-
-
-
-
-
-
-
1
2
-
-
6
-
-
1
-
-
-
1
-
12
2
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
1
2
-
-
-
-
1
-
-
1
-
12
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-