Activating Compound | Comment | Organism | Structure |
---|---|---|---|
CO | 35% activation | Paenarthrobacter nicotinovorans | |
EDTA | 38% activation at 0.05 mM | Paenarthrobacter nicotinovorans | |
KCN | 18% activation at 0.5 mM | Paenarthrobacter nicotinovorans |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cu2+ | 50% inhibition at 0.017 mM | Paenarthrobacter nicotinovorans | |
Hg2+ | 50% inhibition at 0.005 mM | Paenarthrobacter nicotinovorans | |
NaN3 | 50% inhibition at 2 mM | Paenarthrobacter nicotinovorans | |
p-chloromercuribenzoate | 50% inhibition at 0.01 mM | Paenarthrobacter nicotinovorans | |
Zn2+ | 50% inhibition at 0.7 mM | Paenarthrobacter nicotinovorans |
Localization | Comment | Organism | GeneOntology No. | Textmining |
---|---|---|---|---|
soluble | - |
Paenarthrobacter nicotinovorans | - |
- |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
89000 | - |
sucrose density gradient centrifugation | Paenarthrobacter nicotinovorans |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,6-dihydroxypyridine + NADH + O2 | Paenarthrobacter nicotinovorans | - |
2,3,6-trihydroxypyridine + NAD+ + H2O | - |
? |
Organic Solvent | Comment | Organism |
---|---|---|
Acetone | complete loss of activity | Paenarthrobacter nicotinovorans |
Ethanol | stabilizes during preparative manipulations | Paenarthrobacter nicotinovorans |
Glycerol | stabilizes during preparative manipulations | Paenarthrobacter nicotinovorans |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Paenarthrobacter nicotinovorans | - |
formerly Arthrobacter oxidans | - |
Purification (Comment) | Organism |
---|---|
partial | Paenarthrobacter nicotinovorans |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
2.16 | - |
- |
Paenarthrobacter nicotinovorans |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
2,6-dihydroxypyridine + NADH + electron acceptor | methylene blue and 2,6-dichlorophenol-indophenol can act as alternative electron acceptors | Paenarthrobacter nicotinovorans | 2,3,6-trihydroxypyridine + NAD+ + reduced electron acceptor | - |
? | |
2,6-dihydroxypyridine + NADH + O2 | - |
Paenarthrobacter nicotinovorans | 2,3,6-trihydroxypyridine + NAD+ + H2O | - |
? | |
2,6-dihydroxypyridine + NADH + O2 | - |
Paenarthrobacter nicotinovorans | 2,3,6-trihydroxypyridine + NAD+ + H2O | pyridine ring cleavage follows in a second reaction | ? | |
additional information | 2-hydroxypyridine, 3-hydroxypyridine, 4-hydroxypyridine, 2,5-dihydroxypyridine, 2,6-dipicolinic acid, p-hydroxybenzoic acid, nicotinic acid and nicotine are no substrates for this enzyme | Paenarthrobacter nicotinovorans | ? | - |
? |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
30 | - |
- |
Paenarthrobacter nicotinovorans |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
15 | - |
30% loss of activity in 10 min | Paenarthrobacter nicotinovorans |
30 | - |
complete loss of activity in 10 min | Paenarthrobacter nicotinovorans |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Paenarthrobacter nicotinovorans |
pH Stability | pH Stability Maximum | Comment | Organism |
---|---|---|---|
7 | - |
most stable in 50 mM potassium phosphate buffer | Paenarthrobacter nicotinovorans |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Paenarthrobacter nicotinovorans | |
NADH | - |
Paenarthrobacter nicotinovorans | |
NADPH | less effective than NADH | Paenarthrobacter nicotinovorans |