BRENDA - Enzyme Database show
show all sequences of 1.14.13.172

Physicochemical and immunochemical characterization of salicylate 5-hydroxylase, m-hydroxybenzoate 6-hydroxylase and p-hydroxybenzoate 3-hydroxylase from Rhodococcus erythropolis

Suemori, A.; Nakajima, K.; Kurane, R.; Nakamura, Y.; Biotechnol. Lett. 17, 1063-1068 (1995)
No PubMed abstract available

Data extracted from this reference:

Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
4 * 45000, SDS-PAGE
Rhodococcus erythropolis
180000
-
gel filtration
Rhodococcus erythropolis
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Rhodococcus erythropolis
-
-
-
Rhodococcus erythropolis S1
-
-
-
Purification (Commentary)
Commentary
Organism
-
Rhodococcus erythropolis
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
salicylate + NADH + H+ + O2
-
438959
Rhodococcus erythropolis
2,5-dihydroxybenzoate + NAD+ + H2O
-
-
-
?
salicylate + NADH + H+ + O2
-
438959
Rhodococcus erythropolis S1
2,5-dihydroxybenzoate + NAD+ + H2O
-
-
-
?
salicylate + NADPH + H+ + O2
-
438959
Rhodococcus erythropolis
2,5-dihydroxybenzoate + NADP+ + H2O
-
-
-
?
salicylate + NADPH + H+ + O2
-
438959
Rhodococcus erythropolis S1
2,5-dihydroxybenzoate + NADP+ + H2O
-
-
-
?
Subunits
Subunits
Commentary
Organism
homotetramer
4 * 45000, SDS-PAGE
Rhodococcus erythropolis
Cofactor
Cofactor
Commentary
Organism
Structure
NADH
-
Rhodococcus erythropolis
NADPH
-
Rhodococcus erythropolis
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
NADH
-
Rhodococcus erythropolis
NADPH
-
Rhodococcus erythropolis
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
45000
-
4 * 45000, SDS-PAGE
Rhodococcus erythropolis
180000
-
gel filtration
Rhodococcus erythropolis
Purification (Commentary) (protein specific)
Commentary
Organism
-
Rhodococcus erythropolis
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
salicylate + NADH + H+ + O2
-
438959
Rhodococcus erythropolis
2,5-dihydroxybenzoate + NAD+ + H2O
-
-
-
?
salicylate + NADH + H+ + O2
-
438959
Rhodococcus erythropolis S1
2,5-dihydroxybenzoate + NAD+ + H2O
-
-
-
?
salicylate + NADPH + H+ + O2
-
438959
Rhodococcus erythropolis
2,5-dihydroxybenzoate + NADP+ + H2O
-
-
-
?
salicylate + NADPH + H+ + O2
-
438959
Rhodococcus erythropolis S1
2,5-dihydroxybenzoate + NADP+ + H2O
-
-
-
?
Subunits (protein specific)
Subunits
Commentary
Organism
homotetramer
4 * 45000, SDS-PAGE
Rhodococcus erythropolis
Other publictions for EC 1.14.13.172
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
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722507
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1
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1
1
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4
1
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3
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1
3
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-
2
4
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-
-
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-
-
-
4
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
438959
Suemori
-
Physicochemical and immunochem ...
Rhodococcus erythropolis, Rhodococcus erythropolis S1
Biotechnol. Lett.
17
1063-1068
1995
-
-
-
-
-
-
-
-
-
-
2
-
-
5
-
-
1
-
-
-
-
-
4
1
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-
-
-
-
-
2
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-
-
2
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-
-
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-
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2
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1
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4
1
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