Cloned (Comment) | Organism |
---|---|
gene dntB, sequence comparisons | Burkholderia sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
60200 | - |
1 * 60200, SDS-PAGE | Burkholderia sp. |
65000 | - |
gel filtration | Burkholderia sp. |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Burkholderia sp. | P71029 | gene dntB | - |
Burkholderia sp. RASC | P71029 | gene dntB | - |
Purification (Comment) | Organism |
---|---|
native enzme to homogeneity from strain DNT by anion exchange chromatography and gel filtration | Burkholderia sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
4-methyl-5-nitrocatechol + O2 + NADPH + H+ | the enzyme is highly specific for 4-methyl-5-nitrocatechol | Burkholderia sp. | 2-hydroxy-5-methylquinone + nitrite + H2O + NADP+ | - |
? | |
4-methyl-5-nitrocatechol + O2 + NADPH + H+ | the enzyme is highly specific for 4-methyl-5-nitrocatechol | Burkholderia sp. RASC | 2-hydroxy-5-methylquinone + nitrite + H2O + NADP+ | - |
? | |
4-nitrophenol + O2 + NADPH + H+ | 58% of the activity with 4-methyl-5-nitrocatechol | Burkholderia sp. | 4-benzoquinone + nitrite + H2O + NADP+ | - |
? | |
4-nitrophenol + O2 + NADPH + H+ | 58% of the activity with 4-methyl-5-nitrocatechol | Burkholderia sp. RASC | 4-benzoquinone + nitrite + H2O + NADP+ | - |
? | |
additional information | the enzyme has a narrow substrate specificity. 3-Methyl-4-nitrophenol, 3-methyl-4-nitrocatechol, 4-nitrophenol, 3-nitrophenol, and 4-chlorocatechol are no substrates | Burkholderia sp. | ? | - |
? | |
additional information | the enzyme has a narrow substrate specificity. 3-Methyl-4-nitrophenol, 3-methyl-4-nitrocatechol, 4-nitrophenol, 3-nitrophenol, and 4-chlorocatechol are no substrates | Burkholderia sp. RASC | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 60200, SDS-PAGE | Burkholderia sp. |
Synonyms | Comment | Organism |
---|---|---|
4-methyl-5-nitrocatechol oxygenase | - |
Burkholderia sp. |
dntB | - |
Burkholderia sp. |
MNC | - |
Burkholderia sp. |
MNC monooxygenase | - |
Burkholderia sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | the purified enzyme contains 1 mol of FAD per mol of protein | Burkholderia sp. | |
NADH | the enzyme uses either NADH or NADPH as electron donors, and NADPH is the preferred cofactor | Burkholderia sp. | |
NADPH | the enzyme uses either NADH or NADPH as electron donors, and NADPH is the preferred cofactor | Burkholderia sp. |
General Information | Comment | Organism |
---|---|---|
evolution | enzyme DntB contains the highly conserved ADP and flavin adenine dinucleotide (FAD) binding motifs characteristic of flavoprotein hydroxylases | Burkholderia sp. |