Cloned (Comment) | Organism |
---|---|
expressed in Escherichia coli Rosetta pLysS cells | Pseudomonas aeruginosa |
Crystallization (Comment) | Organism |
---|---|
native and seleno-L-methionine-labelled enzyme, hanging drop vapor diffusion method, using 0.7-1.0 M lithium sulfate, 0.1 M sodium citrate pH 5.05.5, 0.5 M ammonium sulfate or 14-17% (w/v) PEG 10 000, 0.1 M ammonium acetate, 0.1 M Bis-Tris pH 5.5 and 23-26% (w/v) PEG 3350, 0.1 M Bis-Tris pH 5.8-6.0, 0.2 M ammonium sulfate | Pseudomonas aeruginosa |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
43600 | - |
- |
Pseudomonas aeruginosa |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-methylphenazine-1-carboxylate + NADH + O2 | Pseudomonas aeruginosa | - |
pyocyanin + NAD+ + CO2 + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Pseudomonas aeruginosa | - |
- |
- |
Purification (Comment) | Organism |
---|---|
Ni-NTA affinity resin column chromatography and Superdex 200 gel filtration | Pseudomonas aeruginosa |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-methylphenazine-1-carboxylate + NADH + O2 | - |
Pseudomonas aeruginosa | pyocyanin + NAD+ + CO2 + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
homodimer | 2 * 43600, calculated from amino acid sequence | Pseudomonas aeruginosa |
Synonyms | Comment | Organism |
---|---|---|
phzS | - |
Pseudomonas aeruginosa |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | - |
Pseudomonas aeruginosa | |
NADH | - |
Pseudomonas aeruginosa |