Protein Variants | Comment | Organism |
---|---|---|
A255C/A293C | mutation results in an apparent Tm increase of 1.5°C | Acinetobacter johnsonii |
A325C/L483C | mutation results in an apparent Tm increase of 1.5°C | Acinetobacter johnsonii |
L323C/A325C | mutation results in an apparent Tm increase of 6°C and a 12fold increased half-life, the stabilizing disulfide bond, L323C/A325C, spans only one residue | Acinetobacter johnsonii |
R534stop | mutation results in an apparent Tm decrease of 1°C | Acinetobacter johnsonii |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.0007 | - |
bicyclo[3.2.0]hept-2-en-6-one | pH 8.5, 20°C, mutant enzyme L323C/A325C | Acinetobacter johnsonii | |
0.0013 | - |
bicyclo[3.2.0]hept-2-en-6-one | pH 8.5, 20°C, wild-type enzyme | Acinetobacter johnsonii | |
0.003 | - |
cyclohexanone | pH 8.5, 20°C, mutant enzyme L323C/A325C | Acinetobacter johnsonii | |
0.0036 | - |
cyclohexanone | pH 8.5, 20°C, wild-type enzyme | Acinetobacter johnsonii | |
0.013 | - |
NADPH | pH 8.5, 20°C, mutant enzyme L323C/A325C | Acinetobacter johnsonii | |
0.016 | - |
NADPH | pH 8.5, 20°C, wild-type enzyme | Acinetobacter johnsonii |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter johnsonii | Q9R2F5 | - |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
bicyclo[3.2.0]hept-2-en-6-one + NADPH + H+ + O2 | the enzyme produces all four possible lactones | Acinetobacter johnsonii | ? | - |
? | |
cyclohexanone + NADPH + H+ + O2 | - |
Acinetobacter johnsonii | hexano-6-lactone + NADP+ + H2O | - |
? |
Synonyms | Comment | Organism |
---|---|---|
CHMO | - |
Acinetobacter johnsonii |
Temperature Stability Minimum [°C] | Temperature Stability Maximum [°C] | Comment | Organism |
---|---|---|---|
37 | - |
Tm-value of mutant enzyme R534stop | Acinetobacter johnsonii |
38 | - |
Tm-value of wild-type enzyme | Acinetobacter johnsonii |
40.5 | - |
Tm-value of mutant enzymes A255C/A293C and A325C/L483C | Acinetobacter johnsonii |
44 | - |
Tm-value of mutant enzyme L323C/A325C | Acinetobacter johnsonii |
Turnover Number Minimum [1/s] | Turnover Number Maximum [1/s] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
4.9 | - |
bicyclo[3.2.0]hept-2-en-6-one | pH 8.5, 20°C, mutant enzyme L323C/A325C | Acinetobacter johnsonii | |
6.1 | - |
cyclohexanone | pH 8.5, 20°C, mutant enzyme L323C/A325C | Acinetobacter johnsonii | |
13.4 | - |
bicyclo[3.2.0]hept-2-en-6-one | pH 8.5, 20°C, wild-type enzyme | Acinetobacter johnsonii | |
14.2 | - |
cyclohexanone | pH 8.5, 20°C, wild-type enzyme | Acinetobacter johnsonii |