Application | Comment | Organism |
---|---|---|
analysis | effective halo-based selection method for mutant proteins using the solubility difference between the substrate (omeprazole sulfide) and product (esomeprazole) | Acinetobacter calcoaceticus |
Crystallization (Comment) | Organism |
---|---|
structure of variant F246Y/K326C/L426F/F432L/T433A/L435S/S438I/F505L complexed with FAD, to 2.2 A resolution | Acinetobacter calcoaceticus |
Protein Variants | Comment | Organism |
---|---|---|
F246Y/K326C/L426F/F432L/T433A/L435S/S438I/F505L | substrate specificity is markedly altered from substrate cyclohexanone toward the desired bulky substrate omeprazole sulfide despite an extremely poor activity | Acinetobacter calcoaceticus |
L143P/F246Y/K269E/K326C/L426F/F432L/T433A/L435S/S438I/F505L/N386S/I388K/M390I/E488K/S489C/W490R | 50fold higher activity with substrate substrate omeprazole than mutant F246Y/K326C/L426F/F432L/T433A/L435S/S438I/F505L | Acinetobacter calcoaceticus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Acinetobacter calcoaceticus | - |
- |
- |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
5-methoxy-2-methylthio-1H-benzimidazole + NADPH + H+ + O2 | poor substrate for wild-type | Acinetobacter calcoaceticus | ? | - |
? | |
cyclohexanone + NADPH + H+ + O2 | - |
Acinetobacter calcoaceticus | hexano-6-lactone + NADP+ + H2O | - |
? | |
omeprazole sulfide + NADPH + H+ + O2 | no substrae for wild-type | Acinetobacter calcoaceticus | (S)-omeprazole + NADP+ + H2O | - |
? | |
thioanisole + NADPH + H+ + O2 | - |
Acinetobacter calcoaceticus | ? | - |
? |