BRENDA - Enzyme Database show
show all sequences of 1.14.13.23

3-Hydroxybenzoate 4-hydroxylase from Pseudomonas testosteroni

Michalover, J.M.; Ribbons, D.W.; Biochem. Biophys. Res. Commun. 55, 888-896 (1973)

Data extracted from this reference:

KM Value [mM]
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.02
-
NADPH
+ 2-fluoro-5-hydroxybenzoate
Comamonas testosteroni
0.03
-
3-hydroxybenzoate
cosubstrate NADPH
Comamonas testosteroni
0.04
-
3,5-Dihydroxybenzoate
cosubstrate NADPH
Comamonas testosteroni
0.05
-
NADPH
cosubstrates 3-hydroxyanthranilate or 3,5-dihydroxybenzoate
Comamonas testosteroni
0.06
-
2,3-Dihydroxybenzoate
cosubstrate NADPH
Comamonas testosteroni
0.07
-
NADPH
cosubstrates 3-hydroxybenzoate or 2,3-dihydroxybenzoate
Comamonas testosteroni
0.12
-
4-Fluoro-3-hydroxybenzoate
cosubstrate NADPH
Comamonas testosteroni
0.14
-
NADPH
+ 2,5-dihydroxybenzoate
Comamonas testosteroni
0.15
-
NADPH
+ 4-fluoro-3-hydroxybenzoate
Comamonas testosteroni
0.5
-
2,5-Dihydroxybenzoate
cosubstrate NADPH
Comamonas testosteroni
3
-
NADH
cosubstrate 3-hydroxybenzoate
Comamonas testosteroni
Molecular Weight [Da]
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
145000
-
sedimentation equilibrium centrifugation
Comamonas testosteroni
Natural Substrates/ Products (Substrates)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3-hydroxybenzoate + [reduced NADPH-hemoprotein reductase] + O2
Comamonas testosteroni
-
3,4-dihydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Organism
Organism
Primary Accession No. (UniProt)
Commentary
Textmining
Comamonas testosteroni
-
-
-
Purification (Commentary)
Commentary
Organism
-
Comamonas testosteroni
Specific Activity [micromol/min/mg]
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
11.8
-
-
Comamonas testosteroni
Substrates and Products (Substrate)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,3-dihydroxybenzoate + NADPH + O2
-
438963
Comamonas testosteroni
?
-
-
-
?
2,5-dihydroxybenzoate + NADPH + O2
-
438963
Comamonas testosteroni
?
-
-
-
?
2-fluoro-5-hydroxybenzoate + NADPH + O2
-
438963
Comamonas testosteroni
?
-
-
-
?
3,5-dihydroxybenzoate + NADPH + O2
-
438963
Comamonas testosteroni
?
-
-
-
?
3-hydroxyanthranilate + NADPH + O2
-
438963
Comamonas testosteroni
?
-
-
-
?
3-hydroxybenzoate + NADH + O2
-
438963
Comamonas testosteroni
3,4-dihydroxybenzoate + NAD+ + H2O
-
-
-
-
3-hydroxybenzoate + [reduced NADPH-hemoprotein reductase] + O2
-
438963
Comamonas testosteroni
3,4-dihydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
4-fluoro-3-hydroxybenzoate + NADPH + O2
-
438963
Comamonas testosteroni
?
-
-
-
?
Temperature Optimum [°C]
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Comamonas testosteroni
Cofactor
Cofactor
Commentary
Organism
Structure
FAD
flavoprotein
Comamonas testosteroni
NADH
poor substitute for NADPH
Comamonas testosteroni
NADPH
-
Comamonas testosteroni
Cofactor (protein specific)
Cofactor
Commentary
Organism
Structure
FAD
flavoprotein
Comamonas testosteroni
NADH
poor substitute for NADPH
Comamonas testosteroni
NADPH
-
Comamonas testosteroni
KM Value [mM] (protein specific)
KM Value [mM]
KM Value Maximum [mM]
Substrate
Commentary
Organism
Structure
0.02
-
NADPH
+ 2-fluoro-5-hydroxybenzoate
Comamonas testosteroni
0.03
-
3-hydroxybenzoate
cosubstrate NADPH
Comamonas testosteroni
0.04
-
3,5-Dihydroxybenzoate
cosubstrate NADPH
Comamonas testosteroni
0.05
-
NADPH
cosubstrates 3-hydroxyanthranilate or 3,5-dihydroxybenzoate
Comamonas testosteroni
0.06
-
2,3-Dihydroxybenzoate
cosubstrate NADPH
Comamonas testosteroni
0.07
-
NADPH
cosubstrates 3-hydroxybenzoate or 2,3-dihydroxybenzoate
Comamonas testosteroni
0.12
-
4-Fluoro-3-hydroxybenzoate
cosubstrate NADPH
Comamonas testosteroni
0.14
-
NADPH
+ 2,5-dihydroxybenzoate
Comamonas testosteroni
0.15
-
NADPH
+ 4-fluoro-3-hydroxybenzoate
Comamonas testosteroni
0.5
-
2,5-Dihydroxybenzoate
cosubstrate NADPH
Comamonas testosteroni
3
-
NADH
cosubstrate 3-hydroxybenzoate
Comamonas testosteroni
Molecular Weight [Da] (protein specific)
Molecular Weight [Da]
Molecular Weight Maximum [Da]
Commentary
Organism
145000
-
sedimentation equilibrium centrifugation
Comamonas testosteroni
Natural Substrates/ Products (Substrates) (protein specific)
Natural Substrates
Organism
Commentary (Nat. Sub.)
Natural Products
Commentary (Nat. Pro.)
Organism (Nat. Pro.)
Reversibility
3-hydroxybenzoate + [reduced NADPH-hemoprotein reductase] + O2
Comamonas testosteroni
-
3,4-dihydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
?
Purification (Commentary) (protein specific)
Commentary
Organism
-
Comamonas testosteroni
Specific Activity [micromol/min/mg] (protein specific)
Specific Activity Minimum [µmol/min/mg]
Specific Activity Maximum [µmol/min/mg]
Commentary
Organism
11.8
-
-
Comamonas testosteroni
Substrates and Products (Substrate) (protein specific)
Substrates
Commentary Substrates
Literature (Substrates)
Organism
Products
Commentary (Products)
Literature (Products)
Organism (Products)
Reversibility
2,3-dihydroxybenzoate + NADPH + O2
-
438963
Comamonas testosteroni
?
-
-
-
?
2,5-dihydroxybenzoate + NADPH + O2
-
438963
Comamonas testosteroni
?
-
-
-
?
2-fluoro-5-hydroxybenzoate + NADPH + O2
-
438963
Comamonas testosteroni
?
-
-
-
?
3,5-dihydroxybenzoate + NADPH + O2
-
438963
Comamonas testosteroni
?
-
-
-
?
3-hydroxyanthranilate + NADPH + O2
-
438963
Comamonas testosteroni
?
-
-
-
?
3-hydroxybenzoate + NADH + O2
-
438963
Comamonas testosteroni
3,4-dihydroxybenzoate + NAD+ + H2O
-
-
-
-
3-hydroxybenzoate + [reduced NADPH-hemoprotein reductase] + O2
-
438963
Comamonas testosteroni
3,4-dihydroxybenzoate + [oxidized NADPH-hemoprotein reductase] + H2O
-
-
-
?
4-fluoro-3-hydroxybenzoate + NADPH + O2
-
438963
Comamonas testosteroni
?
-
-
-
?
Temperature Optimum [°C] (protein specific)
Temperature Optimum [°C]
Temperature Optimum Maximum [°C]
Commentary
Organism
30
-
assay at
Comamonas testosteroni
Other publictions for EC 1.14.13.23
No.
1st author
Pub Med
title
organims
journal
volume
pages
year
Activating Compound
Application
Cloned(Commentary)
Crystallization (Commentary)
Engineering
General Stability
Inhibitors
KM Value [mM]
Localization
Metals/Ions
Molecular Weight [Da]
Natural Substrates/ Products (Substrates)
Organic Solvent Stability
Organism
Oxidation Stability
Posttranslational Modification
Purification (Commentary)
Reaction
Renatured (Commentary)
Source Tissue
Specific Activity [micromol/min/mg]
Storage Stability
Substrates and Products (Substrate)
Subunits
Temperature Optimum [°C]
Temperature Range [°C]
Temperature Stability [°C]
Turnover Number [1/s]
pH Optimum
pH Range
pH Stability
Cofactor
Ki Value [mM]
pI Value
IC50 Value
Activating Compound (protein specific)
Application (protein specific)
Cloned(Commentary) (protein specific)
Cofactor (protein specific)
Crystallization (Commentary) (protein specific)
Engineering (protein specific)
General Stability (protein specific)
IC50 Value (protein specific)
Inhibitors (protein specific)
Ki Value [mM] (protein specific)
KM Value [mM] (protein specific)
Localization (protein specific)
Metals/Ions (protein specific)
Molecular Weight [Da] (protein specific)
Natural Substrates/ Products (Substrates) (protein specific)
Organic Solvent Stability (protein specific)
Oxidation Stability (protein specific)
Posttranslational Modification (protein specific)
Purification (Commentary) (protein specific)
Renatured (Commentary) (protein specific)
Source Tissue (protein specific)
Specific Activity [micromol/min/mg] (protein specific)
Storage Stability (protein specific)
Substrates and Products (Substrate) (protein specific)
Subunits (protein specific)
Temperature Optimum [°C] (protein specific)
Temperature Range [°C] (protein specific)
Temperature Stability [°C] (protein specific)
Turnover Number [1/s] (protein specific)
pH Optimum (protein specific)
pH Range (protein specific)
pH Stability (protein specific)
pI Value (protein specific)
Expression
General Information
General Information (protein specific)
Expression (protein specific)
KCat/KM [mM/s]
KCat/KM [mM/s] (protein specific)
742982
Mulla
-
Biodegradation of 3-chloroben ...
Bacillus sp. (in: Bacteria), Bacillus sp. (in: Bacteria) OS13
J. Environ. Chem. Eng.
4
1423-1431
2016
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
1
1
-
-
-
684919
Chang
Examination and expansion of t ...
Comamonas testosteroni
Biochem. Biophys. Res. Commun.
371
149-153
2008
-
-
1
-
6
-
-
-
-
-
-
2
-
1
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
-
-
6
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
684868
Yoshida
Ligand specificity of MobR, a ...
Comamonas testosteroni, Comamonas testosteroni KH122-3s
Biochem. Biophys. Res. Commun.
362
275-280
2007
1
-
-
-
-
-
1
-
-
-
-
-
-
4
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
1
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-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
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-
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-
-
-
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-
-
-
-
-
-
-
-
-
675417
Hiromoto
Characterization of MobR, the ...
Comamonas testosteroni, Comamonas testosteroni KH122-3s
J. Mol. Biol.
364
863-877
2006
1
-
1
-
-
-
1
-
-
-
-
2
-
4
-
-
-
-
-
1
2
-
4
-
1
-
-
-
-
-
-
2
-
-
-
1
-
1
2
-
-
-
-
1
-
-
-
-
-
2
-
-
-
-
-
1
2
-
4
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
675418
Hiromoto
Crystal structure of 3-hydroxy ...
Comamonas testosteroni, Comamonas testosteroni KH122-3s
J. Mol. Biol.
364
878-896
2006
-
-
1
1
-
-
1
-
-
1
-
2
-
5
-
-
-
1
-
-
-
-
6
2
-
-
-
-
-
-
-
2
-
-
-
-
-
1
2
1
-
-
-
1
-
-
-
1
-
2
-
-
-
-
-
-
-
-
6
2
-
-
-
-
-
-
-
-
-
-
-
-
-
-
390048
Chen
-
Crystallization and further ch ...
Comamonas testosteroni
Res. Commun. Biochem. Cell Mol. Biol.
2
253-274
1998
-
-
-
1
-
-
4
-
-
-
1
1
-
1
-
-
-
-
-
-
1
-
2
1
-
-
-
-
2
-
-
2
-
-
-
-
-
-
2
1
-
-
-
4
-
-
-
-
1
1
-
-
-
-
-
-
1
-
2
1
-
-
-
-
2
-
-
-
-
-
-
-
-
-
438967
Chen
-
Studies on m-hydroxybenzoate 4 ...
Comamonas testosteroni
Res. Commun. Biochem. Cell Mol. Biol.
1
304-322
1997
-
-
-
-
-
-
-
2
-
-
1
1
-
1
-
-
1
-
-
-
1
-
3
1
-
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
-
-
-
-
2
-
-
1
1
-
-
-
1
-
-
1
-
3
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
438965
Mashetty
Degradation of 3-hydroxybenzoi ...
Bacillus sp. (in: Bacteria)
Indian J. Biochem. Biophys.
33
145-148
1996
-
-
-
-
-
-
-
-
-
-
-
1
-
4
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
-
-
-
-
-
-
-
-
-
1
-
-
-
-
-
-
1
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
-
-
438963
Michalover
3-Hydroxybenzoate 4-hydroxylas ...
Comamonas testosteroni
Biochem. Biophys. Res. Commun.
55
888-896
1973
-
-
-
-
-
-
-
11
-
-
1
1
-
2
-
-
1
-
-
-
1
-
8
-
1
-
-
-
-
-
-
3
-
-
-
-
-
-
3
-
-
-
-
-
-
11
-
-
1
1
-
-
-
1
-
-
1
-
8
-
1
-
-
-
-
-
-
-
-
-
-
-
-
-
438964
Kumar
m-Hydroxybenzoate 4-hydroxylas ...
Aspergillus niger
Indian J. Biochem. Biophys.
10
184-190
1973
-
-
-
-
-
1
7
2
-
-
-
1
-
1
-
-
1
-
-
1
-
-
1
-
1
-
-
-
1
-
-
2
-
-
-
-
-
-
2
-
-
1
-
7
-
2
-
-
-
1
-
-
-
1
-
1
-
-
1
-
1
-
-
-
1
-
-
-
-
-
-
-
-
-