Inhibitors | Comment | Organism | Structure |
---|---|---|---|
Cibacron blue | 50% inhibition at 0.04 mM | Micrococcus sp. | |
Cu2+ | 85% inhibition at 0.01 mM, inhibition can be reversed by thiol reagents | Micrococcus sp. | |
Hg2+ | 85% inhibition at 0.01 mM, inhibition can be reversed by thiol reagents | Micrococcus sp. | |
p-chloromercuribenzoate | at low concentrations | Micrococcus sp. |
KM Value [mM] | KM Value Maximum [mM] | Substrate | Comment | Organism | Structure |
---|---|---|---|---|---|
0.063 | - |
3-hydroxybenzoate | cosubstrate NADH | Micrococcus sp. | |
0.095 | - |
NADH | - |
Micrococcus sp. |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
additional information | no requirement for metal ions | Micrococcus sp. |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
67000 | - |
SDS-PAGE | Micrococcus sp. |
70000 | - |
gel filtration | Micrococcus sp. |
Natural Substrates | Organism | Comment (Nat. Sub.) | Natural Products | Comment (Nat. Pro.) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxybenzoate + NADH + O2 | Micrococcus sp. | key role in metabolism of aromatic compounds | 2,5-dihydroxybenzoate + NAD+ + H2O | - |
? |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Micrococcus sp. | - |
- |
- |
Purification (Comment) | Organism |
---|---|
single step purification using substrate-mediated interaction of the enzyme with blue-Sepharose, homogeneity | Micrococcus sp. |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
21.4 | - |
- |
Micrococcus sp. |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
3-hydroxybenzoate + NADH + O2 | strictly specific for 3-hydroxybenzoate | Micrococcus sp. | 2,5-dihydroxybenzoate + NAD+ + H2O | - |
? | |
3-hydroxybenzoate + NADH + O2 | key role in metabolism of aromatic compounds | Micrococcus sp. | 2,5-dihydroxybenzoate + NAD+ + H2O | - |
? | |
additional information | not: salicylate | Micrococcus sp. | ? | - |
? | |
additional information | acts also on a number of analogs of 3-hydroxybenzoate substituted in the 2, 3, 5 and 6 positions | Micrococcus sp. | ? | - |
? | |
additional information | not: p-hydroxybenzoate | Micrococcus sp. | ? | - |
? |
Subunits | Comment | Organism |
---|---|---|
monomer | 1 * 67000, SDS-PAGE | Micrococcus sp. |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8 | - |
- |
Micrococcus sp. |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | flavoprotein | Micrococcus sp. | |
FAD | 1 mol FAD per mol enzyme, exogenous addition of FAD required to regenerate oxidized FAD during catalysis | Micrococcus sp. | |
NADH | - |
Micrococcus sp. | |
NADPH | strict preference for NADH over NADPH | Micrococcus sp. |