General Stability | Organism |
---|---|
5 mM thioglycollate, 5 mM dithiothreitol, 5 mM NADH stabilize component C at 0°C | Methylococcus capsulatus |
Inhibitors | Comment | Organism | Structure |
---|---|---|---|
8-hydroxyquinoline | compound C not inhibited | Methylococcus capsulatus | |
Acetylene | component C not inhibited | Methylococcus capsulatus |
Metals/Ions | Comment | Organism | Structure |
---|---|---|---|
Iron | 1 mol of [2Fe-2S(S-Cys)4]centre per mol protein | Methylococcus capsulatus | |
Iron | 2 g-atom iron | Methylococcus capsulatus | |
Iron | characterization of [Fe2-S2] redox centre of component C | Methylococcus capsulatus |
Molecular Weight [Da] | Molecular Weight Maximum [Da] | Comment | Organism |
---|---|---|---|
additional information | - |
enzyme system consists of 3 protein components A, B, C | Methylococcus capsulatus |
Organism | UniProt | Comment | Textmining |
---|---|---|---|
Methylococcus capsulatus | - |
Bath | - |
Methylococcus capsulatus Bath | - |
Bath | - |
Purification (Comment) | Organism |
---|---|
component C | Methylococcus capsulatus |
Specific Activity Minimum [µmol/min/mg] | Specific Activity Maximum [µmol/min/mg] | Comment | Organism |
---|---|---|---|
6 | - |
purified protein C | Methylococcus capsulatus |
Substrates | Comment Substrates | Organism | Products | Comment (Products) | Rev. | Reac. |
---|---|---|---|---|---|---|
ethene + NAD(P)H + O2 | - |
Methylococcus capsulatus | epoxyethane + NAD(P)+ + H2O | - |
? | |
ethene + NAD(P)H + O2 | - |
Methylococcus capsulatus Bath | epoxyethane + NAD(P)+ + H2O | - |
? | |
methane + NAD(P)H + O2 | - |
Methylococcus capsulatus | methanol + NAD(P)+ + H2O | - |
? | |
methane + NAD(P)H + O2 | - |
Methylococcus capsulatus Bath | methanol + NAD(P)+ + H2O | - |
? |
Subunits | Comment | Organism |
---|---|---|
More | enzyme system consists of 3 protein components A, B, C | Methylococcus capsulatus |
More | component A is a hydroxylase | Methylococcus capsulatus |
Temperature Optimum [°C] | Temperature Optimum Maximum [°C] | Comment | Organism |
---|---|---|---|
45 | - |
assay at | Methylococcus capsulatus |
pH Optimum Minimum | pH Optimum Maximum | Comment | Organism |
---|---|---|---|
8.5 | 9 | component C | Methylococcus capsulatus |
Cofactor | Comment | Organism | Structure |
---|---|---|---|
FAD | protein C, reductase component: contains 1 mol FAD per mol protein | Methylococcus capsulatus | |
NADH | - |
Methylococcus capsulatus |